Injectable Hydrogels by Enzymatic Co‐Crosslinking of Dextran and Hyaluronic Acid Tyramine Conjugates

Wennink, J.W.H.; Niederer, Kerstin; Bochynska, Agnieszka; Teixeira, Liliana S. Moreira; Karperien, Marcel; Feijén, Jan; Dijkstra, Pieter J.

doi:10.1002/masy.201100032

Cited by 30 publications

(30 citation statements)

References 28 publications

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“…Previously, we have shown that Dex‐TA gels very slowly degrade, which may hamper cartilage tissue regeneration. Combining Dex‐TA with HA‐TA at a 50/50 weight ratio resulted in a hydrogel, which degraded in approximately 50 days . It was also shown that the ultimate stress and ultimate strain, as determined by rheology, even exceed that of a Dex‐TA hydrogel at a similar concentration.…”

Section: Resultsmentioning

confidence: 97%

“…Phosphate buffered saline (PBS, pH 7,4) was purchased from B. Braun Co. The Dex‐TA conjugate and HA‐TA conjugate were synthesized using slightly modified procedures as described previously …”

Section: Methodsmentioning

confidence: 99%

“…In previous research, we have shown that in situ forming hydrogels can be prepared by the horseradish peroxidase (HRP)‐mediated (co)‐crosslinking of dextran–tyramine (Dex‐TA) and hyaluronic acid–tyramine (HA‐TA) conjugates . Initial results on placing such in situ forming hydrogels into a cartilage defect indicated adhesion to the surrounding tissue.…”

Section: Introductionmentioning

confidence: 99%

“…In previous research, we have shown that in situ forming hydrogels can be prepared by the horseradish peroxidase (HRP)-mediated (co)-crosslinking of dextran-tyramine (Dex-TA) and hyaluronic acid-tyramine (HA-TA) conjugates. [13][14][15] Initial results on placing such in situ forming hydrogels into a cartilage defect indicated adhesion to the surrounding tissue. We hypothesized that the reaction results in covalent binding of the hydroxyphenyl groups in the polysaccharide tyramine conjugate to collagen tyrosine functional groups in the cartilage matrix.…”

Section: Introductionmentioning

confidence: 99%

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Cartilage adhesive and mechanical properties of enzymatically crosslinked polysaccharide tyramine conjugate hydrogels

Wang

Leber

Buhl

et al. 2014

Polymers for Advanced Techs

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Using a home‐built tensile tester, adhesion and mechanical properties of injectable enzymatically crosslinkable hydrogels were determined by placing the hydrogels in between cartilage surfaces. Dextran–tyramine (Dex‐TA) and hyaluronic acid–tyramine (HA‐TA) conjugates as well as a 50/50 composite material of these polysaccharide conjugates were tested. To integrate the injectable hydrogels with the cartilage tissue, pretreatment of the tissue with a Dex‐TA conjugate solution strongly improved the adhesion. Only failure of the crosslinked hydrogel was observed and not at the hydrogel–tissue interface. Moduli of a Dex‐TA hydrogel are higher than those of a HA‐TA hydrogel, whereas the ultimate strain of the HA‐TA hydrogel was at least three times higher. The Dex‐TA/HA‐TA hydrogel has similar storage and elastic moduli as the Dex‐TA gel and also an ultimate strain of ~30%, similarly as found for the HA‐TA gel. The controlled biodegradability and gelation time of the Dex‐TA/HA‐TA hydrogel, the developed method for strong tissue adhesion of the gel particularly in comparison with fibrin glue, makes this material applicable as an injectable hydrogel for tissue regeneration applications. Copyright © 2014 John Wiley & Sons, Ltd.

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Section: Resultsmentioning

confidence: 97%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Cartilage adhesive and mechanical properties of enzymatically crosslinked polysaccharide tyramine conjugate hydrogels

Wang

Leber

Buhl

et al. 2014

Polymers for Advanced Techs

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“…This also applies tyrosinase, phosphopantetheinyl transferase, lysyl oxidase, plasma amine oxidase, and phosphatases [498]. It made it possible in particular to develop new gels by engrafting tyramine groups into natural and synthetic polymers such as dextran, hyaluronic acid, alginate, cellulose, gelatin, heparin and PEG-PPO [499][500][501][502][503][504][505]509].…”

Section: Selection Of Technologies Of Implantable Devices In Regeneramentioning

confidence: 99%

Introductory Chapter: Multi-Aspect Bibliographic Analysis of the Synergy of Technical, Biological and Medical Sciences Concerning Materials and Technologies Used for Medical and Dental Implantable Devices

Dobrzański¹

2018

Biomaterials in Regenerative Medicine

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Tethering Cells via Enzymatic Oxidative Crosslinking Enables Mechanotransduction in Non‐Cell‐Adhesive Materials

et al. 2021

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proliferation, apoptosis, metabolism, and differentiation. [1,2] However, the variety of strategies to control cell-biomaterial interactions able to transmit mechanical cues from the microenvironment to the cells within engineered tissue constructs has remained limited and near-exclusively relied on cell adhesion. Biomaterials are typically endowed with bioligands that bind to cell adhesion molecules (CAMs; e.g., integrins, cadherins, and selectins). The integrinbinding tripeptide arginine-glycine-aspartic acid (RGD) [3] has been most commonly used as cell-adhesive moiety, but also numerous alternative cell-adhesive nucleotides, [4] peptides, [5] and proteins including antibodies [6] and amyloid-like lysozyme [7] have been explored. However, the constitutively active binding nature of these cell-adhesive bioligands has been associated with adverse effects such as increased fibrous capsule formation and chronic inflammation upon implantation. [8,9] Dynamic material modification strategies that display cell-adhesive bioligands with precise spatial and temporal control have been developed to address this challenge. However, once activated, their cell-adhesive properties are continuously active and can only be annihilated using Cell-matrix interactions govern cell behavior and tissue function by facilitating transduction of biomechanical cues. Engineered tissues often incorporate these interactions by employing cell-adhesive materials. However, using constitutively active cell-adhesive materials impedes control over cell fate and elicits inflammatory responses upon implantation. Here, an alternative cell-material interaction strategy that provides mechanotransducive properties via discrete inducible on-cell crosslinking (DOCKING) of materials, including those that are inherently non-cell-adhesive, is introduced. Specifically, tyramine-functionalized materials are tethered to tyrosines that are naturally present in extracellular protein domains via enzyme-mediated oxidative crosslinking. Temporal control over the stiffness of on-cell tethered 3D microniches reveals that DOCKING uniquely enables lineage programming of stem cells by targeting adhesome-related mechanotransduction pathways acting independently of cell volume changes and spreading. In short, DOCKING represents a bioinspired and cytocompatible cell-tethering strategy that offers new routes to study and engineer cell-material interactions, thereby advancing applications ranging from drug delivery, to cell-based therapy, and cultured meat.The ORCID identification number(s) for the author(s) of this article can be found under https://doi.org/10.1002/adma.202102660.

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Injectable Hydrogels by Enzymatic Co‐Crosslinking of Dextran and Hyaluronic Acid Tyramine Conjugates

Cited by 30 publications

References 28 publications

Cartilage adhesive and mechanical properties of enzymatically crosslinked polysaccharide tyramine conjugate hydrogels

Cartilage adhesive and mechanical properties of enzymatically crosslinked polysaccharide tyramine conjugate hydrogels

Introductory Chapter: Multi-Aspect Bibliographic Analysis of the Synergy of Technical, Biological and Medical Sciences Concerning Materials and Technologies Used for Medical and Dental Implantable Devices

Tethering Cells via Enzymatic Oxidative Crosslinking Enables Mechanotransduction in Non‐Cell‐Adhesive Materials

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