Injectable engineered micro/nano-complexes trigger the reprogramming of bone immune epigenetics

Zhou, Xindie; Xi, Kun; Bian, Jiang; Li, Ziang; Wu, Liang; Tang, Jincheng; Xiong, Chenwei; Yu, Zhentang; Zhang, Junjie; Gu, Yun; Huang, Yong; Cai, Feng; Chen, Liang

doi:10.1016/j.cej.2023.142158

Cited by 9 publications

(8 citation statements)

References 51 publications

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“…After decolorizing the mineralized nodules with cetylpyridinium chloride (Sigma-Aldrich, USA) for 15 min, OD values were measured at 562 nm using a microplate reader. [81] On day 7, the cells were stained using an Alkaline Phosphatase Color Development Kit (Beyotime, C3206, China), and images were obtained under a light microscope. ALP activity was also detected using the Alkaline Phosphatase Assay Kit (Beyotime, P0321S, China).…”

Section: Methodsmentioning

confidence: 99%

“…ALP activity was also detected using the Alkaline Phosphatase Assay Kit (Beyotime, P0321S, China). [81] Quantitative Real-Time Polymerase Chain Reaction (qRT-PCR) and Western Blot: The expression levels of osteogenic genes encoding alkaline phosphatase (ALP), Runt-related transcription factor 2 (RUNX2), Type I collagen (COL-1), and osteocalcin (OCN) in BMSCs were measured via qRT-PCR. BMSCs were seeded on a 6-well culture plate at a density of 1 × 10 4 per well, and 2 mL of osteogenic induction medium and 2 mg of composites were added to each well.…”

Section: Methodsmentioning

confidence: 99%

“…ALP activity was also detected using the Alkaline Phosphatase Assay Kit (Beyotime, P0321S, China). [ 81 ]…”

Section: Methodsmentioning

confidence: 99%

“…After decolorizing the mineralized nodules with cetylpyridinium chloride (Sigma–Aldrich, USA) for 15 min, OD values were measured at 562 nm using a microplate reader. [ 81 ]…”

Section: Methodsmentioning

confidence: 99%

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Injectable Nano‐Micro Composites with Anti‐bacterial and Osteogenic Capabilities for Minimally Invasive Treatment of Osteomyelitis

Lu,

Zhao,

Wang

et al. 2024

Advanced Science

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The effective management of osteomyelitis remains extremely challenging due to the difficulty associated with treating bone defects, the high probability of recurrence, the requirement of secondary surgery or multiple surgeries, and the difficulty in eradicating infections caused by methicillin‐resistant Staphylococcus aureus (MRSA). Hence, smart biodegradable biomaterials that provide effective and precise local anti‐infection effects and can promote the repair of bone defects are actively being developed. Here, a novel nano‐micro composite is fabricated by combining calcium phosphate (CaP) nanosheets with drug‐loaded GelMA microspheres via microfluidic technology. The microspheres are covalently linked with vancomycin (Van) through an oligonucleotide (oligo) linker using an EDC/NHS carboxyl activator. Accordingly, a smart nano‐micro composite called “CaP@MS‐Oligo‐Van” is synthesized. The porous CaP@MS‐Oligo‐Van composites can target and capture bacteria. They can also release Van in response to the presence of bacterial micrococcal nuclease and Ca2+, exerting additional antibacterial effects and inhibiting the inflammatory response. Finally, the released CaP nanosheets can promote bone tissue repair. Overall, the findings show that a rapid, targeted drug release system based on CaP@MS‐Oligo‐Van can effectively target bone tissue infections. Hence, this agent holds potential in the clinical treatment of osteomyelitis caused by MRSA.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

See 2 more Smart Citations

Injectable Nano‐Micro Composites with Anti‐bacterial and Osteogenic Capabilities for Minimally Invasive Treatment of Osteomyelitis

Lu,

Zhao,

Wang

et al. 2024

Advanced Science

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“…During the whole process, BMSCs play an important role as the cornerstone and seed cells. At present, in addition to standard treatments such as bone transplantation, the research on drugs and materials for bone regeneration and repair materials based on stem cells has also shown new vitality [ 8 , 9 ].…”

Section: Introductionmentioning

confidence: 99%

CK1ε drives osteogenic differentiation of bone marrow mesenchymal stem cells via activating Wnt/β-catenin pathway

Yu,

Jiang,

Yin

et al. 2023

Aging

Self Cite

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The treatment of bone defects is a difficult problem in orthopedics. At present, the treatment mainly relies on autologous or allogeneic bone transplantation, which may lead to some complications such as foreign body rejection, local infection, pain, or numbness at the bone donor site. Local injection of conservative therapy to treat bone defects is one of the research hotspots at present. Bone marrow mesenchymal stem cells (BMSCs) can self-renew, significantly proliferate, and differentiate into various types of cells. Although it has been reported that CK1ε could mediate the Wnt/β-catenin pathway, leading to the development of the diseases, whether CK1ε plays a role in bone regeneration through the Wnt/β-catenin pathway has rarely been reported. The purpose of this study was to investigate whether CK1ε was involved in the osteogenic differentiation (OD) of BMSCs through the Wnt/β-catenin pathway and explore the mechanism. We used quantitative reverse transcription-polymerase chain reaction (qRT-qPCR), Western blots, immunofluorescence, alkaline phosphatase, and alizarin red staining to detect the effect of CK1ε on the OD of BMSCs and the Wnt/β-catenin signaling pathway. CK1ε was highly expressed in BMSCs with OD, and our study further demonstrated that CK1ε might promote the OD of BMSCs by activating DLV2 phosphorylation, initiating Wnt signaling downstream, and activating β-catenin nuclear transfer. In addition, by locally injecting a CK1ε-carrying adeno-associated virus (AAV5- CK1ε) into a femoral condyle defect rat model, the overexpression of CK1ε significantly promoted bone repair. Our data show that CK1ε was involved in the regulation of OD by mediating Wnt/β-catenin. This may provide a new strategy for the treatment of bone defects.

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Microfluidic Droplet‐Based Tailorable Porous GelMA Microspheres for 3D Spatio Controllable Cell Coculture

Wang,

Du,

Hao

et al. 2024

Adv Materials Technologies

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For the construction of accurate 3D tissue models in vitro, the 3D coculture model formed by spatio‐controlled distribution of multiple cells is essential. Despite the benefits of porous microspheres with low density, large surface area, and high permeability, exact spatio‐controlled distribution fails to appear in 3D coculture models by using porous microspheres as carriers. Herein, a facile method is proposed to construct a 3D coculture model with heterogeneous cell spatio‐tailored distribution using GelMA microspheres with customizable porosity structure and size based on droplet microfluidics in combination with placeholders. ATPS emulsions, containing two biocompatible immiscible aqueous phases of cell/Alg /dextran (Dex) mixture and polyethylene glycol (PEG) solution, are used as the templates to generate the placeholders. The micron‐sized porous GelMA microspheres are prepared by UV crosslinking of droplets prepared in microfluidic devices, followed by chelation of the placeholders. As a proof of concept, the 3D coculture hepatocellular carcinoma (HCC) model with HepG2 encapsulated outside the pores and HUVEC inside the pores is established. Noteworthy, the cells in the 3D coculture HCC model exhibit greater cell activity and proliferation, enhanced functionalities, and drug resistance than the control group. In conclusion, this straightforward method provides a novel approach for constructing 3D coculture models in vitro.

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Injectable engineered micro/nano-complexes trigger the reprogramming of bone immune epigenetics

Cited by 9 publications

References 51 publications

Injectable Nano‐Micro Composites with Anti‐bacterial and Osteogenic Capabilities for Minimally Invasive Treatment of Osteomyelitis

Injectable Nano‐Micro Composites with Anti‐bacterial and Osteogenic Capabilities for Minimally Invasive Treatment of Osteomyelitis

CK1ε drives osteogenic differentiation of bone marrow mesenchymal stem cells via activating Wnt/β-catenin pathway

Microfluidic Droplet‐Based Tailorable Porous GelMA Microspheres for 3D Spatio Controllable Cell Coculture

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