Injectable composite hydrogels encapsulating gelatin methacryloyl/chitosan microspheres as ARPE-19 cell transplantation carriers

Cheng, Xinsheng; Yin, Panjing; Li, Ting; Luo, Lincong; Yang, Yang; Su, Weiwei; Wang, Yilin; Li, Yanbing; Wang, Yanfang; Wu, Yaobin; Huang, Wenhua

doi:10.1039/d2bm01413k

Cited by 10 publications

(4 citation statements)

References 59 publications

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“…Other studies using materials containing GelMA [ 62 , 63 , 64 ] obtained similar results in live/dead assays, confirming that GelMA-based scaffolds can provide a favorable environment for cartilage-like matrix deposition.…”

Section: Resultssupporting

confidence: 64%

Marine Gelatin-Methacryloyl-Based Hydrogels as Cell Templates for Cartilage Tissue Engineering

et al. 2023

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Marine-origin gelatin has been increasingly used as a safe alternative to bovine and porcine ones due to their structural similarity, avoiding the health-related problems and sociocultural concerns associated with using mammalian-origin materials. Another benefit of marine-origin gelatin is that it can be produced from fish processing-products enabling high production at low cost. Recent studies have demonstrated the excellent capacity of gelatin-methacryloyl (GelMA)-based hydrogels in a wide range of biomedical applications due to their suitable biological properties and tunable physical characteristics, such as tissue engineering applications, including the engineering of cartilage. In this study, fish gelatin was obtained from Greenland halibut skins by an acidic extraction method and further functionalized by methacrylation using methacrylic anhydride, developing a photosensitive gelatin-methacryloyl (GelMA) with a degree of functionalization of 58%. The produced marine GelMA allowed the fabrication of photo-crosslinked hydrogels by incorporating a photoinitiator and UV light exposure. To improve the biological performance, GelMA was combined with two glycosaminoglycans (GAGs): hyaluronic acid (HA) and chondroitin sulfate (CS). GAGs methacrylation reaction was necessary, rendering methacrylated HA (HAMA) and methacrylated CS (CSMA). Three different concentrations of GelMA were combined with CSMA and HAMA at different ratios to produce biomechanically stable hydrogels with tunable physicochemical features. The 20% (w/v) GelMA-based hydrogels produced in this work were tested as a matrix for chondrocyte culture for cartilage tissue engineering with formulations containing both HAMA and CSMA showing improved cell viability. The obtained results suggest these hybrid hydrogels be used as promising biomaterials for cartilage tissue engineering applications.

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Section: Resultssupporting

confidence: 64%

Marine Gelatin-Methacryloyl-Based Hydrogels as Cell Templates for Cartilage Tissue Engineering

et al. 2023

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“…There are many methods for the preparation of microspheres, including the use of microfluidic emulsions, lithography, electrohydrodynamics, and mechanical fragmentation. 26 , 27 , 28 , 29 Compared with other methods, the use of microfluidic chips allows the production of large numbers of uniformly sized microspheres. The principle behind this technique is as follows: At the point of contact between the water and oil phases in the microfluidic chip, the water phase is subjected to shear forces, as well as hydrophobic interactions with the oil phase, thus resulting in the formation of microspheres.…”

Section: Resultsmentioning

confidence: 99%

A modular hydrogel bioink containing microsphere-embedded chondrocytes for 3D-printed multiscale composite scaffolds for cartilage repair

Yin,

Su,

et al. 2023

iScience

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“…This should, however, be further explored, especially given that all of these gels have demonstrated success in other studies. Cell encapsulation in a GelMA composite with chitosan microspheres successfully resulted in well-spread and viable cells in a recent preliminary in vitro study [ 136 ]. Chitosan has a poor aqueous solubility despite its beneficial effects on cell delivery, and encapsulation in the GelMA allowed for the use of chitosan in highly aqueous environments such as the eye [ 136 ].…”

Section: Cell-based Therapies For Ocular Deliverymentioning

confidence: 99%

“…Cell encapsulation in a GelMA composite with chitosan microspheres successfully resulted in well-spread and viable cells in a recent preliminary in vitro study [ 136 ]. Chitosan has a poor aqueous solubility despite its beneficial effects on cell delivery, and encapsulation in the GelMA allowed for the use of chitosan in highly aqueous environments such as the eye [ 136 ]. The varying levels of success in these studies do point out the nuances of hydrogel formation and the importance of the hydrogel composition when considering long-term cell survival and delivery.…”

Section: Cell-based Therapies For Ocular Deliverymentioning

confidence: 99%

Hydrogels in Ophthalmology: Novel Strategies for Overcoming Therapeutic Challenges

Wu,

Akbar,

Giunta

et al. 2023

Materials

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The human eye’s intricate anatomical and physiological design necessitates tailored approaches for managing ocular diseases. Recent advancements in ophthalmology underscore the potential of hydrogels as a versatile therapeutic tool, owing to their biocompatibility, adaptability, and customizability. This review offers an exploration of hydrogel applications in ophthalmology over the past five years. Emphasis is placed on their role in optimized drug delivery for the posterior segment and advancements in intraocular lens technology. Hydrogels demonstrate the capacity for targeted, controlled, and sustained drug release in the posterior segment of the eye, potentially minimizing invasive interventions and enhancing patient outcomes. Furthermore, in intraocular lens domains, hydrogels showcase potential in post-operative drug delivery, disease sensing, and improved biocompatibility. However, while their promise is immense, most hydrogel-based studies remain preclinical, necessitating rigorous clinical evaluations. Patient-specific factors, potential complications, and the current nascent stage of research should inform their clinical application. In essence, the incorporation of hydrogels into ocular therapeutics represents a seminal convergence of material science and medicine, heralding advancements in patient-centric care within ophthalmology.

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Injectable composite hydrogels encapsulating gelatin methacryloyl/chitosan microspheres as ARPE-19 cell transplantation carriers

Abstract: Retinal pigment epithelial (RPE) cell transplantation is being explored as a feasible approach for treating age-related macular degeneration. The low aggregation ability of RPE cell suspensions or microtissues after transplantation...

Cited by 10 publications

References 59 publications

Marine Gelatin-Methacryloyl-Based Hydrogels as Cell Templates for Cartilage Tissue Engineering

Marine Gelatin-Methacryloyl-Based Hydrogels as Cell Templates for Cartilage Tissue Engineering

A modular hydrogel bioink containing microsphere-embedded chondrocytes for 3D-printed multiscale composite scaffolds for cartilage repair

Hydrogels in Ophthalmology: Novel Strategies for Overcoming Therapeutic Challenges

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