Injectable, Cellular-Scale Optoelectronics with Applications for Wireless Optogenetics

Kim, Tae Il; McCall, Jordan G.; Jung, Yei Hwan; Huang, Xian; Siuda, Edward R.; Li, Yuhang; Song, Jizhou; Song, Young Min; Pao, Hsuan An; Kim, Rak Hwan; Li, Chaofeng; Lee, Sung Dan; Song, Il Sun; Shin, Gunchul; Al‐Hasani, Ream; Stanley, Kim; Tan, Ming Jen; Huang, Yonggang; Omenetto, Fiorenzo G.; Rogers, John A.; Bruchas, Michael R.

doi:10.1126/science.1232437

Cited by 1,043 publications

(1,115 citation statements)

References 46 publications

Supporting

Mentioning

1,092

Contrasting

Unclassified

Order By: Relevance

“…IHC was quantified as previously described (Al-Hasani et al, 2013; Kim et al, 2013). 1°Ab rabbit monoclonal α-CaMKIIα (EPR1828) (Abcam; ab92332; 1 : 500 in blocking buffer) and 2°Ab at 1 : 500 in PBS for opto-β 2 AR goat α rabbit IgG Alexa Fluor 488 and for GFP goat α rabbit IgG Alexa Fluor 594.…”

Section: Immunohistochemistrymentioning

confidence: 99%

Chemogenetic and Optogenetic Activation of Gαs Signaling in the Basolateral Amygdala Induces Acute and Social Anxiety-Like States

Siuda

Al‐Hasani

McCall

et al. 2016

Neuropsychopharmacol

Self Cite

View full text Add to dashboard Cite

Anxiety disorders are debilitating psychiatric illnesses with detrimental effects on human health. These heightened states of arousal are often in the absence of obvious threatening cues and are difficult to treat owing to a lack of understanding of the neural circuitry and cellular machinery mediating these conditions. Activation of noradrenergic circuitry in the basolateral amygdala is thought to have a role in stress, fear, and anxiety, and the specific cell and receptor types responsible is an active area of investigation. Here we take advantage of two novel cellular approaches to dissect the contributions of G-protein signaling in acute and social anxiety-like states. We used a chemogenetic approach utilizing the Gαs DREADD (rM3Ds) receptor and show that selective activation of generic Gαs signaling is sufficient to induce acute and social anxiety-like behavioral states in mice. Second, we use a recently characterized chimeric receptor composed of rhodopsin and the β 2 -adrenergic receptor (Opto-β 2 AR) with in vivo optogenetic techniques to selectively activate Gαs β-adrenergic signaling exclusively within excitatory neurons of the basolateral amygdala. We found that optogenetic induction of β-adrenergic signaling in the basolateral amygdala is sufficient to induce acute and social anxiety-like behavior. These findings support the conclusion that activation of Gαs signaling in the basolateral amygdala has a role in anxiety. These data also suggest that acute and social anxiety-like states may be mediated through signaling pathways identical to β-adrenergic receptors, thus providing support that inhibition of this system may be an effective anxiolytic therapy.

show abstract

Section: Immunohistochemistrymentioning

confidence: 99%

Chemogenetic and Optogenetic Activation of Gαs Signaling in the Basolateral Amygdala Induces Acute and Social Anxiety-Like States

Siuda

Al‐Hasani

McCall

et al. 2016

Neuropsychopharmacol

Self Cite

View full text Add to dashboard Cite

show abstract

“…Although successfully applied to live and free-moving animals, this can limit the movement of animal and induce brain damage [127][128][129]. Recent efforts have been made to overcome this limitation with wireless head-mounted systems [130][131][132][133][134]. Although this system made it easy to apply optogenetics to free-moving animals, it is still invasive-wireless headstagemounted systems still need to implant optical fiber into the brain.…”

Section: Optogeneticsmentioning

confidence: 99%

Selective Manipulation of Neural Circuits

Park

Carmel

2016

Neurotherapeutics

View full text Add to dashboard Cite

Unraveling the complex network of neural circuits that form the nervous system demands tools that can manipulate specific circuits. The recent evolution of genetic tools to target neural circuits allows an unprecedented precision in elucidating their function. Here we describe two general approaches for achieving circuit specificity. The first uses the genetic identity of a cell, such as a transcription factor unique to a circuit, to drive expression of a molecule that can manipulate cell function. The second uses the spatial connectivity of a circuit to achieve specificity: one genetic element is introduced at the origin of a circuit and the other at its termination. When the two genetic elements combine within a neuron, they can alter its function. These two general approaches can be combined to allow manipulation of neurons with a specific genetic identity by introducing a regulatory gene into the origin or termination of the circuit. We consider the advantages and disadvantages of both these general approaches with regard to specificity and efficacy of the manipulations. We also review the genetic techniques that allow gain-and loss-of-function within specific neural circuits. These approaches introduce light-sensitive channels (optogenetic) or drug sensitive channels (chemogenetic) into neurons that form specific circuits. We compare these tools with others developed for circuit-specific manipulation and describe the advantages of each. Finally, we discuss how these tools might be applied for identification of the neural circuits that mediate behavior and for repair of neural connections.

show abstract

“…Batteries with biocompatible materials are used for giving energy to these systems 27. Other approaches, such as optogenetics use light triggered ion channels expressed in cell membranes to manipulate cell function 28, 29, 30, 31, 32, 33. These innovative devices open up a window to study biological processes and improve current biomedical tools and techniques 13…”

Section: Introductionmentioning

confidence: 99%

Taking Electrons out of Bioelectronics: From Bioprotonic Transistors to Ion Channels

et al. 2017

View full text Add to dashboard Cite

From cell‐to‐cell communication to metabolic reactions, ions and protons (H+) play a central role in many biological processes. Examples of H+ in action include oxidative phosphorylation, acid sensitive ion channels, and pH dependent enzymatic reactions. To monitor and control biological reactions in biology and medicine, it is desirable to have electronic devices with ionic and protonic currents. Here, we summarize our latest efforts on bioprotonic devices that monitor and control a current of H+ in physiological conditions, and discuss future potential applications. Specifically, we describe the integration of these devices with enzymatic logic gates, bioluminescent reactions, and ion channels.

show abstract

Injectable, Cellular-Scale Optoelectronics with Applications for Wireless Optogenetics

Cited by 1,043 publications

References 46 publications

Chemogenetic and Optogenetic Activation of Gαs Signaling in the Basolateral Amygdala Induces Acute and Social Anxiety-Like States

Chemogenetic and Optogenetic Activation of Gαs Signaling in the Basolateral Amygdala Induces Acute and Social Anxiety-Like States

Selective Manipulation of Neural Circuits

Taking Electrons out of Bioelectronics: From Bioprotonic Transistors to Ion Channels

Contact Info

Product

Resources

About