Initiation of sporulation in B. subtilis is controlled by a multicomponent phosphorelay

Burbulys, D.; Trach, K; Hoch, James A.

doi:10.1016/0092-8674(91)90238-t

Cited by 832 publications

(783 citation statements)

References 26 publications

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“…Spo0A belongs to a large family of phosphorylation-activated signal transduction proteins that regulate adaptative responses in bacteria [1]. It is phosphorylated by a complex phosphorelay that includes several components needed to integrate multiple signals [2]. The spoOA gene product regulates negatively the abrB gene [3,4], a repressor of several genes which transcription is initiated during the transition between exponential growth and stationary phase [5].…”

Section: Introductionmentioning

confidence: 99%

A functional Spo0A is required for maximal aprE expression in Bacillus subtilis

Olmos

Bolaños

Causey³

et al. 1996

FEBS Letters

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The initiation of sporulation in Bacillus subtilis is under control of the transcriptional factor Spo0A. Most Spo0A mutants fail to initiate the sporulation process and aH the sporulation initiated processes such as the synthesis of subtilisin.However, the product of spoOd9V, one of the several spo0A mutants characterized, distinguishes itself in the fact that, while it appears to effectively repress abrB, it fails to activate the spollA operon. The aim of this study was to examine the effect of the spoOA9V mutation on aprE expression and we found that in different genetic backgrounds, the spoOA9V mutation has a negative effect on aprE: :lacZ expression.

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Section: Introductionmentioning

confidence: 99%

A functional Spo0A is required for maximal aprE expression in Bacillus subtilis

Olmos

Bolaños

Causey³

et al. 1996

FEBS Letters

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“…RNA polymerase containing sigma-H transcribes a set of genes that are induced during the first hour of sporulation, many of which were identified on the basis of their requirement for proper spore formation (3,16,24,37). The products of two sigma-H-controlled genes, kinA (spoIIJ) and spo0F, are involved in phosphorylation of the transcription factor Spo0A (1,22). In addition, transcription of spo0A itself is controlled in part by sigma-H (4,22,37), and the sigma-H-controlled promoter upstream of spo0A, Ps, is required for sporulation (29).…”

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confidence: 99%

Characterization of csh203::Tn917lac, a mutation in Bacillus subtilis that makes the sporulation sigma factor sigma-H essential for normal vegetative growth

Hicks

Grossman

1995

J Bacteriol

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spo0H encodes a sigma factor, sigma-H, of RNA polymerase that is required for sporulation in Bacillus subtilis. Null mutations in spo0H block the initiation of sporulation but have no obvious effect on vegetative growth. We have characterized an insertion mutation, csh203::Tn917lac, that makes spo0H essential for normal growth. In otherwise wild-type cells, the csh203::Tn917lac insertion mutation has no obvious effect on cell growth, viability, or sporulation. However, in combination with a mutation in spo0H, the csh203 mutation causes a defect in vegetative growth. The csh203::Tn917lac insertion mutation was found to be located within orf23, the first gene of the rpoD (sigma-A) operon. The transposon insertion separates the major vegetative promoters P1 and P2 from the coding regions of two essential genes, dnaG (encoding DNA primase) and rpoD (encoding the major sigma factor, sigma-A) and leaves these genes under the control of minor promoters, including P4, a promoter controlled by sigma-H. The csh203 insertion mutation caused a 2-to 10-fold increase in expression of promoters recognized by RNA polymerase containing sigma-H. The increased expression of genes controlled by sigma-H in the csh203 single mutant, as well as the growth defect of the csh203 spo0H double mutant, was due to effects on rpoD and not to a defect in orf23 or dnaG.

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“…Many signals that regulate sporulation do so by affecting the accumulation of Spo0AϳP. Spo0A obtains phosphate from a set of proteins called the phosphorelay (2). A balance of kinases and phosphatases regulates phosphate input into the phosphorelay and the phosphorylation state of Spo0A (3,(30)(31)(32).…”

mentioning

confidence: 99%

Control of Sporulation Gene Expression in Bacillus subtilis by the Chromosome Partitioning Proteins Soj (ParA) and Spo0J (ParB)

Quisel

Grossman

2000

J Bacteriol

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Two chromosome partitioning proteins, Soj (ParA) and Spo0J (ParB), regulate the initiation of sporulation in Bacillus subtilis. In a spo0J null mutant, sporulation is inhibited by the action of Soj. Soj negatively regulates expression of several sporulation genes by binding to the promoter regions and inhibiting transcription. All of the genes known to be inhibited by Soj are also activated by the phosphorylated form of the transcription factor Spo0A (Spo0AϳP). We found that, in a spo0J null mutant, Soj affected sporulation, in part, by decreasing the level of Spo0A protein. Soj negatively regulated transcription of spo0A and associated with the spo0A promoter region in vivo. Expression of spo0A from a heterologous promoter in a spo0J null mutant restored Spo0A levels and partly bypassed the sporulation and gene expression defects. Soj did not appear to significantly affect phosphorylation of Spo0A. Thus, in the absence of Spo0J, Soj inhibits sporulation and sporulation gene expression by inhibiting accumulation of the activator protein Spo0A and by acting downstream of Spo0A to inhibit gene expression directly.Bacillus subtilis can sporulate in response to starvation and high cell density. Sporulation involves an asymmetric cell division and requires DNA replication and chromosome segregation. Spo0A is a critical transcription factor in early sporulation. Cells initiate sporulation when they accumulate a threshold amount of phosphorylated Spo0A (Spo0AϳP) (3,6,12). Many signals that regulate sporulation do so by affecting the accumulation of Spo0AϳP. Spo0A obtains phosphate from a set of proteins called the phosphorelay (2). A balance of kinases and phosphatases regulates phosphate input into the phosphorelay and the phosphorylation state of Spo0A (3,(30)(31)(32).Two proteins involved in chromosome partitioning, Soj and Spo0J, also regulate the initiation of sporulation, perhaps in response to chromosome structure or partitioning (4,14,28). Soj and Spo0J are members of the ParA-SopA and ParB-SopB protein families, respectively. Many ParA-and ParB-type proteins are encoded on low-copy-number plasmids and are required for accurate plasmid partitioning (29, 42). Chromosome-encoded family members have also been identified, several of which function in chromosome partitioning (14,22,26). Members of the ParA-SopA families can act as transcriptional repressors and are themselves often regulated by members of the ParB-SopB family (7,9,24,27,36).Spo0J is required both for accurate chromosome partitioning and for sporulation (14). Spo0J binds to a series of sites (parS sites) in the origin-proximal 20% of the chromosome (22), clustering them into a large focus (10,21,23). Defects in spo0J perturb chromosome partitioning. Cultures of spo0J null mutant cells produce approximately 1% anucleate cells, a frequency approximately 20-to 100-fold greater than that of wildtype cells (14).A null mutation in spo0J also causes an approximately 300-fold defect in sporulation relative to wild-type cells (14,28,34). spo0J null mutants ar...

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Initiation of sporulation in B. subtilis is controlled by a multicomponent phosphorelay

Cited by 832 publications

References 26 publications

A functional Spo0A is required for maximal aprE expression in Bacillus subtilis

A functional Spo0A is required for maximal aprE expression in Bacillus subtilis

Characterization of csh203::Tn917lac, a mutation in Bacillus subtilis that makes the sporulation sigma factor sigma-H essential for normal vegetative growth

Control of Sporulation Gene Expression in Bacillus subtilis by the Chromosome Partitioning Proteins Soj (ParA) and Spo0J (ParB)

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