A functional Spo0A is required for maximal <i>aprE</i> expression in <i>Bacillus subtilis</i>

Olmos, Jorge; Bolaños, Víctor Martínez; Causey, S C; Ferrari, Eugenio; Bollvar, Francisco; Valle, Fernando

doi:10.1016/0014-5793(96)00070-1

Cited by 20 publications

(11 citation statements)

References 24 publications

(28 reference statements)

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“…2). These results are in good agreement with the fact that a major control of aprE expression occurs at initiation of transcription through the AbrB\SpoOA switch (Olmos et al, 1996).…”

Section: Construction and Transcriptional Control Of Apre-lacz Fusionssupporting

confidence: 88%

The aprE leader is a determinant of extreme mRNA stability in Bacillus subtilis

2000

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The Bacillus subtilis aprE gene encodes subtilisin, an extracellular proteolytic enzyme produced in stationary phase. The authors examined the stability of aprE mRNA and aprE leader-lacZ fusion mRNA. Both mRNAs were found to be unusually stable, with half-lives longer than 25 min, demonstrating that the aprE leader contains a determinant for extreme mRNA stability. The half-lives were the same in growing and stationary-phase cells. This contrasts with the findings of O. Resnekov et al. (1990) [Proc Natl Acad Sci U S A 87, 8355-8359], which suggested a growth-phase-dependent mechanism for decay of aprE mRNA. The discrepancy is explained by the techniques used. Substitution of two bases or deletion of 25 nucleotides in the aprE leader led to a major difference in its predicted secondary structure and resulted in a fivefold reduction of the half-life of aprE mRNA. The authors also determined the halflife of amyE mRNA, which encodes α-amylase, another stationary-phase, excreted enzyme and found it to be around 5 min. This shows that extreme stability is not a general property of stationary-phase mRNAs encoding excreted enzymes.

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“…2). These results are in good agreement with the fact that a major control of aprE expression occurs at initiation of transcription through the AbrB\SpoOA switch (Olmos et al, 1996).…”

Section: Construction and Transcriptional Control Of Apre-lacz Fusionssupporting

confidence: 88%

The aprE leader is a determinant of extreme mRNA stability in Bacillus subtilis

2000

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“…We compared the promoter strength of cry3Aa with that of the wellknown aprE promoter (Ogura et al, 2004). It has been reported that protein expression from the aprE promoter increased significantly in cells with the degU32 genetic background (Olmos et al, 1996). Our results showed that lacZ expression induced by the cry3Aa promoter was 3.86-fold higher than the expression induced by the aprE promoter in wild-type and 3.58-fold less than that of aprE promoter in degU32 cells (Table 2).…”

Section: Screening Of Cry Promoterssupporting

confidence: 34%

Development of a stationary phase-specific autoinducible expression system in Bacillus subtilis

Lee

Pan

Park

et al. 2010

Journal of Biotechnology

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“…In agreement with this observation, most genes that are positively regulated by DegU-P are known to be repressed by other regulatory proteins during exponential growth. Transcription of amyE is repressed by CcpA in the presence of glucose (Henkin et al 1991) and aprE is transcriptionally repressed by the transient phase regulators ScoC (Henner et al 1988a;Kallio et al 1991), AbrB (Ferrari et al 1988;Olmos et al 1996) and SinR (Olmos et al 1997). Negative regulation by ScoC has also been described for nprE (Kallio et al 1991), and expression of bglS was found to be regulated in response to the level of GTP in the cell, most probably also mediated by AbrB (Stu¨lke et al 1993).…”

Section: Resultsmentioning

confidence: 86%

Bacillus subtilis functional genomics: genome-wide analysis of the DegS-DegU regulon by transcriptomics and proteomics

et al. 2002

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The DegS-DegU two-component regulatory system of Bacillus subtilis controls various processes that characterize the transition from the exponential to the stationary growth phase, including the induction of extracellular degradative enzymes, expression of late competence genes and down-regulation of the sigma(D) regulon. The degU32(Hy) mutation stabilizes the phosphorylated form of DegU (DegU-P), resulting in overproduction of several extracellular degradative enzymes. In this study, the pleiotropic DegS-DegU regulon was characterized by combining proteomic and transcriptomic approaches. A comparative analysis of wild-type B. subtilis and the degU32(Hy) mutant grown in complex medium was performed during the exponential and in the stationary growth phase. Besides genes already known to be under the control of DegU-P, novel putative members of this regulon were identified. Although the degU32(Hy) mutant is assumed to contain high levels of phosphorylated DegU in the exponential as well as in the stationary growth phase, many genes known to be positively regulated by DegU-P did not show enhanced expression in the mutant strain during exponential growth. This is consistent with the fact that most genes belonging to the DegS-DegU regulon are subject to multiple regulation; this is also reflected in the strong stationary-phase induction of these genes in the mutant strain. As expected, during the exponential growth phase, the sigma(D) regulon was expressed at significantly lower levels in the degU32(Hy) mutant than in the wild type.

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A functional Spo0A is required for maximal aprE expression in Bacillus subtilis

Cited by 20 publications

References 24 publications

The aprE leader is a determinant of extreme mRNA stability in Bacillus subtilis

The aprE leader is a determinant of extreme mRNA stability in Bacillus subtilis

Development of a stationary phase-specific autoinducible expression system in Bacillus subtilis

Bacillus subtilis functional genomics: genome-wide analysis of the DegS-DegU regulon by transcriptomics and proteomics

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