Initiation of somatic embryogenesis in coconut (Cocos nucifera L.)

Buffard-Morel, Jacqueline; Verdeil, Jean‐Luc; Dussert, Stéphane; Magnaval, C.; Huet, C.; Grosdemange, Frédérique

doi:10.1007/978-94-011-0433-3_18

Cited by 7 publications

(6 citation statements)

References 7 publications

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“…The cotyledon of coconut is described as a haustorial like tissue developed during embryogenesis (Buffard-Morel et al, 1995). As the embryo develops, the cotyledon becomes the dominant tissue, overwhelming the growth of root and shoot primordium (Haccius and Philip, 1979).…”

Section: Discussionmentioning

confidence: 99%

Phytochemical composition and antioxidant activity of coconut cotyledon

Kannaian

Edwin²,

Rajagopal³

et al. 2020

Heliyon

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Coconut tree (Cocos nucifera L.), a perennial, monocot tree, belonging to the family Arecaceae, is distributed through the tropics. Bioactivities of coconut water, husk fiber, oil, flowers, spadix and mesocarp of coconut fruit are widely reported. However, there is no study on cotyledon of coconut. In this study, carbohydrates, proteins, lipids, phenols, flavonoids, tannins, alkaloids and antioxidants were quantified in hot and cold percolated extracts of coconut cotyledon. Further, the antioxidant activity was studied using 2,2-diphenyl-1-picrylhydrazyl (DPPH); ferric reducing antioxidant power (FRAP); ferric thiocyanate (FTC); thiobarbituric acid (TBA); nitric oxide (NO) radical scavenging and β-carotene bleaching assays. Among the secondary metabolites, only cardiac glycosides were detected. Methanolic extraction by cold percolation extracted high content of secondary metabolites and exhibited significant antioxidant activity in DPPH, FRAP, NO and β-carotene bleaching assays, with EC 50 of 0.12, 6.43, 16.21 and 8.09 mg/ml respectively. The chloroform extracts recorded high lipid content and scavenged the radicals in FTC (EC 50 13.31 mg/ml) and TBA (EC 50 9.21 mg/ml) assays. The study recommends extraction of compounds using methanol through cold percolation. The cotyledon of coconut is found to be a potent nutritive source equivalent to the endosperm.

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Section: Discussionmentioning

confidence: 99%

Phytochemical composition and antioxidant activity of coconut cotyledon

Kannaian

Edwin²,

Rajagopal³

et al. 2020

Heliyon

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“…It has been proposed that haustorial growth is a key event for the reliable germination of somatic embryos (Buffard-Morel et al 1995). Previous experiments in our laboratories with coconut zygotic embryos failed to show promotion of haustorial growth, even in the presence of various growth factors (López-Villalobos 2002).…”

Section: Introductionmentioning

confidence: 71%

Lauric acid improves the growth of zygotic coconut (Cocos nucifera L.) embryos in vitro

López‐Villalobos

Dodds

Hornung

2011

Plant Cell Tiss Organ Cult

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Coconuts (Cocos nucifera L.) germinating in situ make use of both sucrose and fatty acids, notably lauric acid, as sources of energy and carbon, but current tissue culture methods routinely include only sucrose in the culture medium. The aim of the experiments was to establish whether lauric acid could improve the growth and development of zygotic coconut embryos in culture. The culture medium of zygotic embryos was adjusted to give various concentrations of sucrose and lauric acid. The concentration of free lauric acid was increased at specific times of the culture. At the end of the experiments, plantlet growth was measured. Added at day zero, lauric acid inhibited germination. Added at 60 or 75 days, lauric acid (75 lM, unbound concentration) showed a marked stimulation of plantlet growth and development. When 14 C-labelled lauric acid was used, radioactivity was incorporated mainly into longer chain fatty acids of complex lipids, notably of the phospholipid fraction. Supplementation at these times may mimic conditions in situ and suggests that the supply of fatty acids may represent a physiological requirement for continued growth. The experiments with radioactive lauric acid confirm that it provides carbon for the synthesis of new structural lipids. The method may provide a means of improving the development of coconut somatic embryos in future.

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“…Somatic embryogenesis regeneration technique has been applied broadly in many species of plants, such as coconut (Buffard-Morel et al 1995), banana (Sidha et al 2007), pruatjan (Roostika et al 2007), strawberry (Kordestani and Karami 2008), coffee (Oktavia et al 2003;Gatica et al 2008), ginger (Lincy et al 2009), longan (Roostika et al 2009), orchid (Rianawati et al 2009), and pineapple (Sripaoraya et al 2003;Firoozabady and Moy 2004). However, information on the whole step of somatic embryogenesis regeneration on pineapple is limited, especially at the early stage of embryogenic cell initiation.…”

Section: Introductionmentioning

confidence: 99%

The Effect of Picloram and Light on Somatic Embryogenesis Regeneration of Pineapple

Roostika

Khumaida

Mariska

et al. 2012

Indones. J. Agric. Sci.

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Smooth Cayenne is the largest pineapple type cultivated in Indonesia, but its vegetative planting materials for mass propagation are limited. Somatic embryogenesis is a potential method to be applied. The aim of this study was to investigate the somatic embryogenesis regeneration under the effect of picloram and light. Callus formation was induced by picloram (21, 41 and 62 μM) added with 9 μM thidiazuron. The calli were transferred onto MS or Bac medium enriched with N-organic compounds with or without addition of 21 μM picloram under dark or light condition. The compact calli were subcultured onto MS medium supplemented with 4.65 μM kinetin, while the friable calli were transferred onto BIG medium (modified MS + 1.1 μM benzyl adenine + 0.9 μM indole butyric acid + 0.09 μM giberelic acid) or B medium (MS + 0.018 mM benzyl adenine). The results showed that the events of somatic embryogenesis were started from cell polarization, asymmetrical division, proembryo formation as embryogenic tissues and friable embryogenic tissues, and embryo development. The best treatment for callus induction was 21 μM picloram. The addition of 21 μM picloram on N-organic enriched medium and the use of light condition proliferated embryogenic calli. The N-organic enriched Bac medium and light condition yielded the highest number of mature somatic embryos (17 embryos per explant in 2 months). The B medium was better than BIG medium to develop somatic embryos from friable embryogenic tissues. The somatic embryogenesis method presented is potential for pineapple mass propagation and artificial seed production.Abstrak Bahasa IndonesiaSmooth Cayenne merupakan kultivar nenas yang banyak dibudidayakan di Indonesia, namun ketersediaan benih untuk perbanyakan massal masih terbatas. Embriogenesis somatikadalah metode yang potensial untuk produksi bibit secara massal. Tujuan penelitian adalah untuk mempelajari pengaruh pikloram dan pencahayaan terhadap regenerasi embriogenesis somatik nenas. Kalus diinduksi menggunakan pikloram (21, 41, dan 62 μM) dan penambahan thidiazuron 9 μM. Selanjutnya, kalus dipindahkan ke media MS atau Bac yang diperkaya dengan senyawa N-organik dengan atau tanpa penambahan pikloram 21 μM dalam kondisi gelap atau dengan pencahayaan. Kalus kompak disubkultur pada media MS yang mengandung kinetin 4,65 μM, sedangkan kalus remah dipindahkan ke media BIG (MS modifikasi + bensil adenin 1.1 μM + indole butyric acid 0,9 μM + giberelic acid 0,09 μM) atau media B (MS + bensil adenin 0,018 μM). Hasil penelitian menunjukkan bahwa tahapan embriogenesis somatik diawali dengan polarisasi sel, pembelahan asimetris, pembentukan proembrio sebagai jaringan embriogenik dan jaringan embriogenik remah, serta perkembangan embrio. Perlakuan terbaik untuk induksi kalus adalah pikloram 21 μM. Penambahan pikloram 21 μM pada media yang diperkaya dengan senyawa N-organik mampu meningkatkan jumlah kalus embriogenik. Media Bac yang diperkaya senyawa N-organik dan kondisi pencahayaan menghasilkan jumlah embrio somatik dewasa terbanyak (17 embrio per eksplan dalam 2 bulan). Media B lebih baik daripada media BIG untuk regenerasi embrio somatik dari jaringan embriogenik remah. Metode embriogenesis somatik yang dihasilkan dari penelitian ini berpotensi diterapkan untuk perbanyakan massal dan produksi benih nenas.

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Initiation of somatic embryogenesis in coconut (Cocos nucifera L.)

Cited by 7 publications

References 7 publications

Phytochemical composition and antioxidant activity of coconut cotyledon

Phytochemical composition and antioxidant activity of coconut cotyledon

Lauric acid improves the growth of zygotic coconut (Cocos nucifera L.) embryos in vitro

The Effect of Picloram and Light on Somatic Embryogenesis Regeneration of Pineapple

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