Initiation of microspore embryogenesis by stress

Touraev, Alisher; Vicente, Óscar; Heberle‐Bors, Erwin

doi:10.1016/s1360-1385(97)89951-7

Cited by 256 publications

(169 citation statements)

References 38 publications

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“…Henry (1998) cita que a duplicação cromossômica via fusão é um evento pouco comum, uma vez que a freqüência de sincronia no processo mitótico é baixa. Touraev et al (1997) citam que a fusão nuclear seria um caminho assumido por grãos embriogênicos em cultura, resultando em duplo-haplóides espontâneos como, também, observado por Sunderland & Evans (1980) em cevada. Sunderland et al (1974) comentam que esses eventos de duplicação espontânea, em processos de regeneração de calos, são importantes, pois a duplicação do complemento cromossômico pela colchicina apresenta efeitos deletérios.…”

Section: Resultsunclassified

“…Nessas espécies a alteração na rota gametofítica do desenvolvimento dos andrósporos e grãos de pólen jovens está intimamente relacionada com a capacidade embriogênica e conseqüente produção de plantas haplóides, a partir destes grãos. As plantas então originadas, completamente homozigotas, são muito importantes em estudos sobre genética e melhoramento vegetal (Maheshwari et al 1982, Touraev et al 1997, portanto, essenciais para programas de desenvolvimento agrícola. Segundo Grando & Moraes-Fernandes (1997) …”

Section: Introductionunclassified

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Grãos de pólen anômalos no clone CP76 de Anacardium occidentale L. (Anacardiaceae)

Oliveira¹,

Mariath²,

Bueno³

2001

Rev. bras. Bot.

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-(Anomalous pollen grains in the clone CP76 of Anacardium occidentale L. (Anacardiaceae)). Anomalies of male gametophyte development was observed during anther development investigation in the clone CP76 of Anacardium occidentale. Androspores that stop development before the first pollen mitosis, and grains that show male gametophyte anomalies in early stage of development, was observed. The anomalous first mitosis generate two identical cells, or quite, or grains where cell plate was partially formed. Nuclear fusion occurs in those cells where partial cell walls were formed. The interruption of development before first pollen mitosis occurs in high frequency, on the other hand, the anomalies related with the first pollen mitosis are very rare.RESUMO -(Grãos de pólen anômalos no clone CP76 de Anacardium occidentale L. (Anacardiaceae)). Durante a investigação do desenvolvimento das anteras e grãos de pólen no clone CP76 de Anacardium occidentale foram observadas anomalias relacionadas ao desenvolvimento do gametófito masculino. Foram observados andrósporos que interromperam seu desenvolvimento momentos antes da primeira mitose, além de grãos que apresentaram anomalias relacionadas à fase inicial da gametogênese masculina. A primeira mitose anômala produziu grãos de pólen binucleados com células idênticas, ou quase, e ainda grãos onde a parede celular, oriunda da primeira mitose, foi incompletamente formada. Fusão nuclear foi observada nos grãos onde a parede celular foi incompletamente formada. A interrupção no desenvolvimento é muito freqüente, ao contrário das anomalias relacionadas à primeira mitose, de ocorrência muito rara.

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Section: Resultsunclassified

Section: Introductionunclassified

Grãos de pólen anômalos no clone CP76 de Anacardium occidentale L. (Anacardiaceae)

Oliveira¹,

Mariath²,

Bueno³

2001

Rev. bras. Bot.

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“…It slows down the degradation processes, affects the cytoskeleton, activates Ca 2? pathways, and stimulates expression of small heat shock proteins (Touraev et al 1997;Zoriniants et al 2005;Dubas et al 2010). Among protein species that were accumulated in anthers of the triticale DH lines studied after LT treatment were those involved in carbon metabolism.…”

Section: Discussionmentioning

confidence: 99%

“…Microspore embryogenesis (ME) is one of the examples of such induced 'totipotency' taking place in male gametophytic cells triggered by stress toward sporophytic developmental pathway (Touraev et al 1997). In the literature, the term 'ME' is often used synonymously with 'androgenesis' or 'pollen embryogenesis' and also in this paper it refers to the process taking place in in vitro cultured anthers in which the cells of male gametophyte were reprogrammed towards embryo-like structures (ELS) production.…”

Section: Introductionmentioning

confidence: 99%

“…The progressively growing economic significance of this cereal, resulting from the combination of valuable features such as high yield potential and good grain quality with high tolerance to unfavourable environmental conditions has focused much interest on the identification of genetic factors controlling its embryogenic potential and effective production of DH lines (Gonzalez et al 2005;Krzewska et al 2012;_ Zur et al , 2013. The milestone in DH techniques development was the discovery that the efficiency of ME can be significantly increased by the application of a stress treatment (Lichter 1985;Touraev et al 1997). In triticale, a long (2-3 weeks) low temperature (LT) treatment applied to tillers is the most effective stress factor (Immonen and Robinson 2000;Pauk et al 2003;Wędzony 2003;_ Zur et al 2008_ Zur et al , 2009.…”

Section: Introductionmentioning

confidence: 99%

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Identification of proteins related to microspore embryogenesis responsiveness in anther cultures of winter triticale (×Triticosecale Wittm.)

et al. 2017

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For a better understanding of the physiological background of microspore embryogenesis (ME), the protein profile was analyzed in four winter triticale DH lines, which show extremely different embryogenic potential. The analysis were conducted with anthers at the phase of development optimal for ME induction and then after low temperature (LT, 3 weeks at 4°C) MEinducing tillers treatment. The sub-proteome of anthers was mapped by two-dimensional gel electrophoresis (2-DE). The protein species significantly more abundant (at least 2-fold) in responsive DH lines after LT treatment were chosen for identification by MALDI-TOF/TOF analysis. In total, 31 protein species were successfully identified as involved in the determination of microspore competence, stress response and in the regulation of ME induction. Microspore competence required sufficient energy supply and efficient system of cell protection that determine survival under prolonged LT stress treatment. LT stress was associated with increased accumulation of proteins typical for cell defence against oxidative stress (e.g., L-ascorbate peroxidase), chaperons (e.g., HSP70) and other enzymes/factors ensuring protein biosynthesis, stability and active cell divisions. Also here, effective cell defence required undisturbed energy supply. Among proteins that accumulated differentially in accordance with microspore embryogenic potential again the most important role seems to be played by the enzymes ensuring energy production and determining ability of plant stress adaptation. Two protein species (enolase, 12S storage protein), proposed earlier as candidates for markers of embryogenesis in other in vitro plant culture systems confirmed their utility for triticale anther cultures.

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Haploid Plant Production: Pollen/Anther/Ovule Culture

Veilleux

2000

Encyclopedia of Cell Technology

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Initiation of microspore embryogenesis by stress

Cited by 256 publications

References 38 publications

Grãos de pólen anômalos no clone CP76 de Anacardium occidentale L. (Anacardiaceae)

Grãos de pólen anômalos no clone CP76 de Anacardium occidentale L. (Anacardiaceae)

Identification of proteins related to microspore embryogenesis responsiveness in anther cultures of winter triticale (×Triticosecale Wittm.)

Haploid Plant Production: Pollen/Anther/Ovule Culture

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