Initial state of the<i>Drosophila</i>eye before dorsoventral specification is equivalent to ventral

Singh, Amit; Choi, Kwang‐Wook

doi:10.1242/dev.00864

Cited by 61 publications

(119 citation statements)

References 54 publications

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“…Development 140 (9) 2000; Singh and Choi, 2003). The genetic separation of emc and wg in the dorsal half of the retina may stem from the need to activate Wg signaling independently of emc.…”

Section: Research Articlementioning

confidence: 99%

Extramacrochaetae imposes order on the Drosophila eye by refining the activity of the Hedgehog signaling gradient

Spratford

Kumar

2013

Development

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The compound eye of Drosophila melanogaster is configured by a differentiating wave, the morphogenetic furrow, that sweeps across the eye imaginal disc and transforms thousands of undifferentiated cells into a precisely ordered repetitive array of 800 ommatidia. The initiation of the furrow at the posterior margin of the epithelium and its subsequent movement across the eye field is controlled by the activity of the Hedgehog (Hh) signaling pathway. Differentiating photoreceptors that lie behind the furrow produce and secrete the Hh morphogen, which is captured by cells within the furrow itself. This leads to the stabilization of the full-length form of the zinc-finger transcription factor Cubitus interruptus (Ci155), the main effector of Hh signaling. Ci155 functions as a transcriptional activator of a number of downstream targets, including decapentaplegic (dpp), a TGFβ homolog. In this report, we describe a mechanism that is in place within the fly retina to limit Hh pathway activity within and ahead of the furrow. We demonstrate that the helix-loop-helix (HLH) protein Extramacrochaetae (Emc) regulates Ci155 levels. Loss of emc leads to an increase in Ci155 levels, nuclear migration, apical cell constriction and an acceleration of the furrow. We find that these roles are distinct from the bHLH protein Hairy (H), which we show restricts atonal (ato) expression ahead of the furrow. Secondary furrow initiation along the dorsal and ventral margins is blocked by the activity of the Wingless (Wg) pathway. We also show that Emc regulates and cooperates with Wg signaling to inhibit lateral furrow initiation.

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Section: Research Articlementioning

confidence: 99%

Extramacrochaetae imposes order on the Drosophila eye by refining the activity of the Hedgehog signaling gradient

Spratford

Kumar

2013

Development

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“…It is not clear when dorsal-ventral differences are first established, as transcripts encoding the GATA family transcription factor Pannier (Pnr) are present in the dorsalmost region of the embryonic eye disc (41), but a pnr-GAL4 line does not drive UAS-GFP expression in the dorsal eye disc until early second instar (42). wg-lacZ is expressed in the dorsal peripodial membrane and margin cells beginning in the first instar (6,35,43); at least at later stages, dorsal wg expression is dependent on pnr (41).…”

Section: Wg Patterns the Dorsal-ventral Axis In The Eye Discmentioning

confidence: 99%

“…Both Ser and its upstream activator Lobe are required for ventral eye development and growth (42). Lobe and Ser have been shown to repress ventral wg expression during the second instar; their absence results in ectopic Wg signaling that triggers cell death and loss of the ventral eye (49).…”

Section: Wg Patterns the Dorsal-ventral Axis In The Eye Discmentioning

confidence: 99%

Wingless Signaling in Drosophila Eye Development

Legent

Treisman

2008

Methods in Molecular Biology

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The Drosophila eye disc gives rise to both the retina and the surrounding head capsule. The secreted morphogen Wingless (Wg) plays an important role in subdividing the disc between these two tissues; wg is expressed in the anterior lateral margins, regions that will give rise to the head capsule, and high levels of Wg signaling promote this fate. Wg is expressed earlier and more strongly in the dorsal than the ventral margin, and also contributes to specifying dorsal identity. In addition, Wg signaling can cause overgrowth of eye disc cells. Finally, at pupal stages, wg expression surrounds the eye and a gradient of Wg establishes several distinct peripheral cell fates. We describe how to generate clones of cells mutant for genes encoding components of the Wg signaling pathway in the eye disc and examine their effects on photoreceptor differentiation by antibody staining.

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“…Anteroposterior compartments are formed prior to dorsoventral (DV) patterning in most imaginal discs (Cohen 1993). However, in the eye disc, the formation of the DV axis is the first lineage restriction event (Baker 1978;Dominguez and de Celis 1998;Singh and Choi 2003). Therefore, it is important to understand how DV patterning and growth is regulated in the early eye disc.…”

mentioning

confidence: 99%

“…Apart from the genes expressed in a domain-specific pattern, there are genes that are expressed ubiquitously in the eye but exhibit a domain-specific function (Chern and Choi 2002;Singh and Choi 2003;Singh et al 2004). For example, Lobe (L), which encodes a novel protein, is expressed ubiquitously in the eye disc but is required only for ventral eye development.…”

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confidence: 99%

Genetic Interaction of Lobe With Its Modifiers in Dorsoventral Patterning and Growth of the Drosophila Eye

et al. 2005

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Dorsoventral (DV) patterning is essential for growth of the Drosophila eye. Recent studies suggest that ventral is the default state of the early eye, which depends on Lobe (L) function, and that the dorsal fate is established later by the expression of the dorsal selector gene pannier (pnr). However, the mechanisms of regulatory interactions between L and dorsal genes are not well understood. For studying the mechanisms of DV patterning in the early eye disc, we performed a dominant modifier screen to identify additional genes that interact with L. The criterion of the dominant interaction was either enhancement or suppression of the L ventral eye loss phenotype. We identified 48 modifiers that correspond to 16 genes, which include fringe ( fng), a gene involved in ventral eye patterning, and members of both Hedgehog (Hh) and Decapentaplegic (Dpp) signaling pathways, which promote L function in the ventral eye. Interestingly, 29% of the modifiers (6 enhancers and 9 suppressors) identified either are known to interact genetically with pnr or are members of the Wingless (Wg) pathway, which acts downstream from pnr. The detailed analysis of genetic interactions revealed that pnr and L mutually antagonize each other during second instar of larval development to restrict their functional domains in the eye. This time window coincides with the emergence of pnr expression in the eye. Our results suggest that L function is regulated by multiple signaling pathways and that the mutual antagonism between L and dorsal genes is crucial for balanced eye growth.

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Initial state of theDrosophilaeye before dorsoventral specification is equivalent to ventral

Cited by 61 publications

References 54 publications

Extramacrochaetae imposes order on the Drosophila eye by refining the activity of the Hedgehog signaling gradient

Extramacrochaetae imposes order on the Drosophila eye by refining the activity of the Hedgehog signaling gradient

Wingless Signaling in Drosophila Eye Development

Genetic Interaction of Lobe With Its Modifiers in Dorsoventral Patterning and Growth of the Drosophila Eye

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