Wingless Signaling in Drosophila Eye Development

Legent, Kevin; Treisman, Jessica E.

doi:10.1007/978-1-60327-469-2_12

Cited by 31 publications

(24 citation statements)

References 95 publications

(70 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…One of these is the signaling molecule Wingless (Wg), which is secreted from the anterior lateral regions of the eye disc and from cells surrounding the eye field (Baker, 1988;Tomlinson, 2003), and acts to promote head capsule differentiation and restrict eye development (Heslip et al, 1997;Ma and Moses, 1995;Royet and Finkelstein, 1996;Treisman and Rubin, 1995;Legent and Treisman, 2008). Wg counteracts Ey activity by repressing the expression of the retinal determination genes eya, sine oculis (so) and dachshund (dac), which are direct targets of Ey (Baonza and Freeman, 2002;Niimi et al, 1999;Pappu et al, 2005;Ostrin et al, 2006).…”

Section: Introductionmentioning

confidence: 99%

The transcriptional co-factor Chip acts with LIM-homeodomain proteins to set the boundary of the eye field in Drosophila

et al. 2010

Self Cite

View full text Add to dashboard Cite

SUMMARYDevelopment involves the establishment of boundaries between fields specified to differentiate into distinct tissues. The Drosophila larval eye-antennal imaginal disc must be subdivided into regions that differentiate into the adult eye, antenna and head cuticle. We have found that the transcriptional co-factor Chip is required for cells at the ventral eye-antennal disc border to take on a head cuticle fate; clones of Chip mutant cells in this region instead form outgrowths that differentiate into ectopic eye tissue. Chip acts independently of the transcription factor Homothorax, which was previously shown to promote head cuticle development in the same region. Chip and its vertebrate CLIM homologues have been shown to form complexes with LIM-homeodomain transcription factors, and the domain of Chip that mediates these interactions is required for its ability to suppress the eye fate. We show that two LIM-homeodomain proteins, Arrowhead and Lim1, are expressed in the region of the eye-antennal disc affected in Chip mutants, and that both require Chip for their ability to suppress photoreceptor differentiation when misexpressed in the eye field. Loss-of-function studies support the model that Arrowhead and Lim1 act redundantly, using Chip as a co-factor, to prevent retinal differentiation in regions of the eye disc destined to become ventral head tissue.

show abstract

Section: Introductionmentioning

confidence: 99%

The transcriptional co-factor Chip acts with LIM-homeodomain proteins to set the boundary of the eye field in Drosophila

et al. 2010

Self Cite

View full text Add to dashboard Cite

show abstract

“…Activation of Wg signaling within the eye field (e.g., in sgg clones; Figures 1E and 5C) transforms retinal tissue into ectopic head cuticle (Legent and Treisman 2008). Clones homozygous for our mutation 8B12 similarly resulted in cuticle outgrowths protruding from the retina of adult flies ( Figure 5A).…”

Section: A Novel Mutation Upregulates Wg Target Genesmentioning

confidence: 91%

“…Third instar eye and wing imaginal discs were dissected and stained according to (Legent and Treisman 2008). Antibodies used were: rabbit anti-b2galactosidase (1:5000, Cappel), chicken anti-GFP (1:500, Aves), mouse anti-Flag (1:500, Sigma), rabbit anti-Ato (1:5000; Jarman et al 1994), guinea pig anti-Sens (1:1000; Nolo et al 2000) mouse anti-N ECD (1:10), mouse anti-Dl ECD (1:10), mouse anti-Arm (1:10), mouse anti-Smo (1:1000), mouse anti-Ptc (1:10), mouse anti-En (1:2), rat anti-Ci (1:2), rat anti-Elav (1:100) (Developmental Studies Hybridoma Bank, University of Iowa), rabbit anti-EGFR CTER (1:500; Rodrigues et al 2005), guinea pig anti-dMyc (1:100, a gift from Ginés Morata), and mouse anti-Omb (1:100; Shen et al 2010).…”

Section: Immunohistochemistrymentioning

confidence: 99%

“…The morphogen Wingless (Wg) promotes head cuticle formation by cells at the margins of the eye disc, excluding retinal differentiation from these regions (Legent and Treisman 2008). Wg prevents ectopic initiation and progression of the MF (Ma and Moses 1995;Treisman and Rubin 1995) by repressing drumstick, which encodes an activator of hh (Bras-Pereira et al 2006); retinal determination genes such as eyes absent (eya) (Baonza and Freeman 2002;Kenyon et al 2003); and dpp (Heslip et al 1997).…”

mentioning

confidence: 99%

See 1 more Smart Citation

A Screen for X-Linked Mutations Affecting Drosophila Photoreceptor Differentiation Identifies Casein Kinase 1α as an Essential Negative Regulator of Wingless Signaling

et al. 2012

Self Cite

View full text Add to dashboard Cite

The Wnt and Hedgehog signaling pathways are essential for normal development and are misregulated in cancer. The casein kinase family of serine/threonine kinases regulates both pathways at multiple levels. However, it has been difficult to determine whether individual members of this family have distinct functions in vivo, due to their overlapping substrate specificities. In Drosophila melanogaster, photoreceptor differentiation is induced by Hedgehog and inhibited by Wingless, providing a sensitive system in which to identify regulators of each pathway. We used a mosaic genetic screen in the Drosophila eye to identify mutations in genes on the X chromosome required for signal transduction. We recovered mutations affecting the transcriptional regulator CREB binding protein, the small GTPase dynamin, the cytoskeletal regulator Actin-related protein 2, and the protein kinase Casein kinase 1a. Consistent with its reported function in the b-Catenin degradation complex, Casein Kinase 1a mutant cells accumulate b-Catenin and ectopically induce Wingless target genes. In contrast to previous studies based on RNA interference, we could not detect any effect of the same Casein Kinase 1a mutation on Hedgehog signaling. We thus propose that Casein kinase 1a is essential to allow b-Catenin degradation and prevent inappropriate Wingless signaling, but its effects on the Hedgehog pathway are redundant with other Casein kinase 1 family members.

show abstract

“…Third-instar eye and wing discs were dissected and stained as described previously (Legent and Treisman, 2008). Ovaries were stained as described previously (Miura et al, 2006).…”

Section: Immunohistochemistrymentioning

confidence: 99%

Drosophila Vps4 promotes Epidermal growth factor receptor signaling independently of its role in receptor degradation

2015

Self Cite

View full text Add to dashboard Cite

Endocytic trafficking of signaling receptors is an important mechanism for limiting signal duration. Components of the Endosomal Sorting Complexes Required for Transport (ESCRT), which target ubiquitylated receptors to intra-lumenal vesicles (ILVs) of multivesicular bodies, are thought to terminate signaling by the epidermal growth factor receptor (EGFR) and direct it for lysosomal degradation. In a genetic screen for mutations that affect Drosophila eye development, we identified an allele of Vacuolar protein sorting 4 (Vps4), which encodes an AAA ATPase that interacts with the ESCRT-III complex to drive the final step of ILV formation. Photoreceptors are largely absent from Vps4 mutant clones in the eye disc, and even when cell death is genetically prevented, the mutant R8 photoreceptors that develop fail to recruit surrounding cells to differentiate as R1-R7 photoreceptors. This recruitment requires EGFR signaling, suggesting that loss of Vps4 disrupts the EGFR pathway. In imaginal disc cells mutant for Vps4, EGFR and other receptors accumulate in endosomes and EGFR target genes are not expressed; epistasis experiments place the function of Vps4 at the level of the receptor. Surprisingly, Vps4 is required for EGFR signaling even in the absence of Shibire, the Dynamin that internalizes EGFR from the plasma membrane. In ovarian follicle cells, in contrast, Vps4 does not affect EGFR signaling, although it is still essential for receptor degradation. Taken together, these findings indicate that Vps4 can promote EGFR activity through an endocytosis-independent mechanism.

show abstract

Wingless Signaling in Drosophila Eye Development

Cited by 31 publications

References 95 publications

The transcriptional co-factor Chip acts with LIM-homeodomain proteins to set the boundary of the eye field in Drosophila

The transcriptional co-factor Chip acts with LIM-homeodomain proteins to set the boundary of the eye field in Drosophila

A Screen for X-Linked Mutations Affecting Drosophila Photoreceptor Differentiation Identifies Casein Kinase 1α as an Essential Negative Regulator of Wingless Signaling

Drosophila Vps4 promotes Epidermal growth factor receptor signaling independently of its role in receptor degradation

Contact Info

Product

Resources

About