Initial induction and subsequent reduction of α2u-globulin in urine and serum of mature male rats after repeated intraperitoneal injections of (anti)estrogen

Nagahori, Hirohisa; Komai, Koichiro; Tomigahara, Yoshitaka; Saito, Koichi; Isobe, Naohiko; Kaneko, Hideo

doi:10.1016/s0300-483x(00)00465-0

Cited by 9 publications

(5 citation statements)

References 29 publications

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“…Furthermore, with a molecular weight of 47 kDa and 46 kDa, respectively, our proteomics identified significantly increased levels of Alpha-1-antichymotrypsin and This result may have a twofold explanation; first, there could be a bias due to the sample size as females are more represented than males (39 vs. 36 subjects respectively). Second, it is known that differences in concentration of alpha 2 globulins exist between women and men and are mainly related to hormonal influence, as reported in rats [35] and humans [36]. The classification accuracy of the proposed multiplexed test is higher than that of the validated biomarker carbohydrate antigen 19-9 (CA19-9).…”

Section: Resultsmentioning

confidence: 92%

Multiplexed Detection of Pancreatic Cancer by Combining a Nanoparticle-Enabled Blood Test and Plasma Levels of Acute-Phase Proteins

Caputo

Coppola

Quagliarini

et al. 2022

Cancers

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The development of new tools for the early detection of pancreatic ductal adenocarcinoma (PDAC) represents an area of intense research. Recently, the concept has emerged that multiplexed detection of different signatures from a single biospecimen (e.g., saliva, blood, etc.) may exhibit better diagnostic capability than single biomarkers. In this work, we develop a multiplexed strategy for detecting PDAC by combining characterization of the nanoparticle (NP)-protein corona, i.e., the protein layer that surrounds NPs upon exposure to biological fluids and circulating levels of plasma proteins belonging to the acute phase protein (APPs) family. As a first step, we developed a nanoparticle-enabled blood (NEB) test that employed 600 nm graphene oxide (GO) nanosheets and human plasma (HP) (5% vol/vol) to produce 75 personalized protein coronas (25 from healthy subjects and 50 from PDAC patients). Isolation and characterization of protein corona patterns by 1-dimensional (1D) SDS-PAGE identified significant differences in the abundance of low-molecular-weight corona proteins (20–30 kDa) between healthy subjects and PDAC patients. Coupling the outcomes of the NEB test with the circulating levels of alpha 2 globulins, we detected PDAC with a global capacity of 83.3%. Notably, a version of the multiplexed detection strategy run on sex-disaggregated data provided substantially better classification accuracy for men (93.1% vs. 77.8%). Nanoliquid chromatography tandem mass spectrometry (nano-LC MS/MS) experiments allowed to correlate PDAC with an altered enrichment of Apolipoprotein A-I, Apolipoprotein D, Complement factor D, Alpha-1-antichymotrypsin and Alpha-1-antitrypsin in the personalized protein corona. Moreover, other significant changes in the protein corona of PDAC patients were found. Overall, the developed multiplexed strategy is a valid tool for PDAC detection and paves the way for the identification of new potential PDAC biomarkers.

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Section: Resultsmentioning

confidence: 92%

Multiplexed Detection of Pancreatic Cancer by Combining a Nanoparticle-Enabled Blood Test and Plasma Levels of Acute-Phase Proteins

Caputo

Coppola

Quagliarini

et al. 2022

Cancers

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“…The expression of alpha-2u globulin (Spot 8) was also downregulated by TCE. This protein is expressed primarily in the mature male rat liver and excreted in the urine, and is absent from or only marginally detectable in female rats 19,20 . Expression of the alpha-2u globulin gene in male rat liver has been changed under oxidative stress to affect developmental and multihormonal regulation 21 .…”

Section: Resultsmentioning

confidence: 99%

Proteomic analysis of rat liver proteins differentially induced by trichloroethylene

Park¹,

Nam²,

Ryu

et al. 2010

BioChip J

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Trichloroethylene (TCE), a volatile and lipophilic solvent widely used in industrial degreasing applications, is believed to be toxic in humans. To identify possible protein biomarkers of early TCE-induced hepatotoxicity, we treated rats with a single dose of TCE equivalent to 10% of LD 50 and analyzed their liver proteomes 72 h after dosing. We identified eight liver proteins that exhibited a TCE-induced change in expression level of at least 2-fold; three of these proteins were upregulated and the rest were downregulated. Because these proteins changed significantly in abundance in the absence of notable hepatotoxicity in vivo, they may be useful as one of the candidates of early predictive biomarkers of TCE exposure in rats.

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“…Accordingly, TDI dose was 0.05 mg/kg bw/day, NOAEL dose was 5 mg/kg bw/day and LOAEL dose was 120 mg/kg bw/day (European Food Safety Authority (EFSA), 2006). In experimental animal (rat and mouse) studies, BPA was administered by gavage at between 1 and 1,250 mg kg −1 day −1 doses (Kim et al, 2001; Morrissey et al, 1987; Shelby, 2008) and by intraperitoneal route up to 100 mg kg −1 day −1 dose (Kovanecz et al, 2013; Nagahori et al, 2001).…”

Section: Methodsmentioning

confidence: 99%

Impact of Bisphenol A on neural tube development in 48‐hr chicken embryos

Atay

Ertekin

Bozkurt

et al. 2020

Birth Defects Research

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Objectives: Bisphenol A (BPA) is one of the most heavily produced chemicals in the world. BPA is involved in the production of many substances such as cosmetics, various foodstuffs, toys, personal care products, detergents and plastic bottles all that are frequently used in daily life. Depending on BPA exposure, sexual maturation and reproductive function, and bone and brain development are adversely affected. The aim of this study is to investigate the possible effects of BPA on the development of the nervous system and neural tube in 48-hr chicken embryos. Methods: Thirty specific pathogen-free (SPF) fertilized eggs were used in the study. SPF eggs were placed in the incubator and divided into three groups at 28 hr of incubation; control, BPA 1 and BPA 2 (10 eggs in each group). At this stage of incubation, two different doses of BPA were injected subblastodermically with the Hamilton microinjector. At the end of 48 hr of incubation, all eggs were opened and embryos were dissected and separated from the embryonic membrane. All embryos were evaluated morphologically and histopathologically. Results: As the BPA dose increased, delays in the development of the nervous system and midline closure increased in the early period of chicken embryos. Depending on the dose, it was found that the embryo's crown-rump length and somite number decreased (p < .05). Conclusion: It was determined that BPA application on early chicken embryos adversely affected neural tube development. It was also found to delay midline closure.

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Initial induction and subsequent reduction of α2u-globulin in urine and serum of mature male rats after repeated intraperitoneal injections of (anti)estrogen

Cited by 9 publications

References 29 publications

Multiplexed Detection of Pancreatic Cancer by Combining a Nanoparticle-Enabled Blood Test and Plasma Levels of Acute-Phase Proteins

Multiplexed Detection of Pancreatic Cancer by Combining a Nanoparticle-Enabled Blood Test and Plasma Levels of Acute-Phase Proteins

Proteomic analysis of rat liver proteins differentially induced by trichloroethylene

Impact of Bisphenol A on neural tube development in 48‐hr chicken embryos

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