Proteomic analysis of rat liver proteins differentially induced by trichloroethylene

Abstract

Trichloroethylene (TCE), a volatile and lipophilic solvent widely used in industrial degreasing applications, is believed to be toxic in humans. To identify possible protein biomarkers of early TCE-induced hepatotoxicity, we treated rats with a single dose of TCE equivalent to 10% of LD 50 and analyzed their liver proteomes 72 h after dosing. We identified eight liver proteins that exhibited a TCE-induced change in expression level of at least 2-fold; three of these proteins were upregulated and the rest were downregulated. Because these proteins changed significantly in abundance in the absence of notable hepatotoxicity in vivo, they may be useful as one of the candidates of early predictive biomarkers of TCE exposure in rats.

Toxicoproteomic identification of TiO2nanoparticle-induced protein expression changes in mouse brain

“…After isoelectric focusing, the strips were soaked twice for 20 min each in equilibration buffer (6 M urea, 2% w/v SDS, 20% v/v glycerol, 5 mM tributylphosphine, 2.5% acrylamide, 0.01% bromophenol blue, 0.375 M Tris-HCl, pH 8.8). The seconddimension SDS-PAGE was performed in 8Á16% gradient polyacrylamide gels without a stacking gel, at 108C for 2 h at 5 mA/gel and then at 18 mA/gel (Bartkowiak et al 2009;Choi et al 2009;Jeon et al 2011;Park et al 2010). Protein bands were visualized by staining with Coomassie Brilliant Blue G-250.…”

Ethylbenzene-induced differential protein profiles in rat liver

“…Expression of the alpha-2u globulin gene was also changed under oxidative stress to affect developmental and multihormonal regulation 28 . Expression of the alpha2u globulin was also down-regulated in rat liver exposed to trichloroethylene (TCE) 29 .…”

“…To get total protein, the liver tissue was removed through a midline abdominal incision, frozen in liquid nitrogen and stored until analysis 29 .…”

“…Livers were solubilized using tip-probe sonication in modified lysis buffer [7 M urea, 2 M thiourea, 4.5% (w/v) CHAPS, 40 mM Tris, 100 mM DTE] containing a protease inhibitor cocktail as reported previously 29 . The solubilized tissue was centrifuged at 12,000×g for 50 min at 15� C. The supernatant fractions were transferred to new tubes, assayed for protein concentration, aliquoted, and stored at -70� C until analyzed.…”

Biomarker analysis of rat livers exposed to different toxic pollutants (VOCs and PAHs) using an antibody array

“…PAH toxicity is quite structurally dependent on isomers 7 Gene expression profiling studies on VOCs and PAHs have been reported 10,11 . Proteomic tools such as two-dimensional gel electrophoresis (2D-GE) and mass spectrometry analysis have been applied for the analysis of rat livers 12,13 .…”