Initial in vitro interaction of osteoblasts with nano-porous alumina

Karlsson, Marjam; Pålsgård, Eva; Wilshaw, Peter R.; Silvio, L. Di

doi:10.1016/s0142-9612(03)00146-7

Cited by 182 publications

(129 citation statements)

References 35 publications

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“…However, other studies show no effect of the surface chemistry (Ref 6, 11). Another important factor linked to the cell adhesion is the wettability of the surface, which can have either a direct effect on adhesion by promoting the cell contact with the surface or an indirect one by promoting the protein unfolding at the surface (Ref [12][13][14]. The effect of protein type on cell adhesion is certainly very important.…”

Section: Alkaline Phosphatase Activity and Cell Differentiationmentioning

confidence: 99%

Development of Osteoblast Colonies on New Bioactive Coatings

Legoux

Chellat

Lima

et al. 2006

Journal of Thermal Spray Technology

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Section: Alkaline Phosphatase Activity and Cell Differentiationmentioning

confidence: 99%

Development of Osteoblast Colonies on New Bioactive Coatings

Legoux

Chellat

Lima

et al. 2006

Journal of Thermal Spray Technology

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“…The material in question has been evaluated as a potential bone implant coating [23] and as a stent coating for drug delivery [24]. In both cases showing favorable tissue compatibility, thus leading us to believe that it not only is suitable as a model substrate for investigating protein and cellular organization on nanoscale level, but may also be used as an implant material with a great potential for controlling type and magnitude of cellular and molecular events at the tissue-implant interface.…”

Section: Introductionmentioning

confidence: 99%

Influence of nanoporesize on platelet adhesion and activation

Ferraz

Carlsson

Hong

et al. 2008

J Mater Sci: Mater Med

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In this study we have evaluated the influence of biomaterial nano-topography on platelet adhesion and activation. Nano-porous alumina membranes with pore diameters of 20 and 200 nm were incubated with whole blood and platelet rich plasma. Platelet number, adhesion and activation were determined by using a coulter hematology analyzer, scanning electron microscopy, immunocytochemical staining in combination with light microscopy and by enzyme immunoassay. Special attention was paid to cell morphology, microparticle generation, Pselectin expression and b-TG production. Very few platelets were found on the 200 nm alumina as compared to the 20 nm membrane. The platelets found on the 20 nm membrane showed signs of activation such as spread morphology and protruding filipodia as well as P-selectin expression. However no microparticles were detected on this surface. Despite the fact that very few platelets were found on the 200 nm alumina in contrast to the 20 nm membrane many microparticles were detected on this surface. Interestingly, all microparticles were found inside circular shaped areas of approximately 3 lm in diameter. Since this is the approximate size of a platelet we speculate that this is evidence of transient, non-adherent platelet contact with the surface, which has triggered platelet microparticle generation. To the authors knowledge, this is the first study that demonstrates how nanotexture can influence platelet microparticle generation. The study highlights the importance of understanding molecular and cellular events on nano-level when designing new biomaterials.

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“…In the case of nano-porous AAO membranes the dominant surface feature sensed by the cells, is the pore size of the membrane. The size sensing ability of osteoblast cells was confirmed by a study by Karlsson et al [27]. This study found that by varying the pore size, it was indeed possible to control the secretion of specific protein species from the cell wall to the substrate, which in turn influenced cellular attachment, differentiation and mineralization taking place.…”

Section: Introductionmentioning

confidence: 68%

Biocompatibility Of Synthesised Nano-Porous Anodic Aluminium Oxide Membranes For Use As A Cell Culture Substrate For Madin-Darby Canine Kidneys Cells: A Preliminary Study

Poinern¹

2012

J Tissue Sci Eng

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In this study we investigate for the first time the biomedical potential of using a membrane made from anodic aluminium oxide (AAO) for culturing the Madin-Darby Canine Kidney (MDCK) epithelial cell line. Nano-porous aluminium oxide membranes exhibit interesting properties such as high porosity, which allows the exchange of molecules and nutrients across the membrane and can be made with highly specific pore sizes that can be preselected by adjusting the controlling parameters of a temperature controlled two-step anodization process. The cellular response and interactions of the MDCK cell line with the synthesised nano-porous AAO membrane, a commercially available membrane and a glass control were assessed by investigating cell adhesion, morphology and proliferation. The number of viable cells proliferating over the surface of each respective membrane was quantified over a 72 h period and revealed that synthesised alumina membrane was at least comparable to the glass control substrate. Furthermore, both optical and electron microscopy investigations revealed distinct evidence of focal adhesion sites on the nano-porous membranes and the penetration of cellular extensions into the pore structure. The results of the study indicate that nano-porous AAO membranes have the potential to become practical cell culture substrates with the ability to enhance adhesion and proliferation of MDCK cells.

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Initial in vitro interaction of osteoblasts with nano-porous alumina

Cited by 182 publications

References 35 publications

Development of Osteoblast Colonies on New Bioactive Coatings

Development of Osteoblast Colonies on New Bioactive Coatings

Influence of nanoporesize on platelet adhesion and activation

Biocompatibility Of Synthesised Nano-Porous Anodic Aluminium Oxide Membranes For Use As A Cell Culture Substrate For Madin-Darby Canine Kidneys Cells: A Preliminary Study

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