Initial Analysis on the Characteristics and Synthesis of Exopolysaccharides from Sclerotium rolfsii with Different Sugars as Carbon Sources

Abstract

Scleroglucan is widely used in the food and chemical industries because of its good rheological property, stability, and emulsification activity. To investigate the influence of different carbon sources on the properties and synthesis of exopolysaccharides (EPS), the three EPSs (GEPS, glucose was used as the carbon source; LEPS, lactose was used as the carbon source; and SEPS, sucrose was used as the carbon source) were determined, respectively. It was found that the yield and viscosity of exopolysaccharides were different. When sucrose and glucose were used as the carbon sources, the viscosity and yield of EPS were both higher than lactose. The scanning electron microscopy (SEM) images showed that the three EPSs had different morphologies, but the monosaccharide analysis showed that they were all composed of glucose units. Fourier transform infrared spectroscopy (FT-IR) proved that there were no additional substituents for the three EPSs. Furthermore, the high performance liquid chromatography (HPLC) results showed that SEPS and LEPS had two fractions. Through the analysis of proteomics data, there were few differences in the metabolic pathways between GEPS and SEPS, but a significant difference between LEPS and SEPS. Our study provides a theoretical basis and reference for understanding the biosynthesis of exopolysaccharides and the development of different types of EPS products.

Fungal exopolysaccharides: Properties, sources, modifications, and biomedical applications

“…(Natalia A. Castillo, Valdez, & Fariña, 2015;Geller & Yan, 2020;Jindal & Singh Khattar, 2018;Kırtel, Avşar, Erkorkmaz, & Öner, 2017;Subhadip Mahapatra & Debdulal Banerjee, 2013a;Microbial Polymers, Applications, and Ecological Perspectives, 2021;PubChem, 2021b;J. Song et al, 2020;Valdez, Delgado, & Fariña, 2021)…”

Elucidation and Regulation of Polyphenols in the Smoking Process of Shanxi Aged Vinegar

“…The moisture content in the smoking Pei was determined via the drying method. The reducing sugars in the samples were determined by high-performance liquid chromatography [ 23 ] (Agilent 1260, Agilent Technologies Inc., Santa Clara, CA, USA).…”

Transcriptomics and Metabolomics Analysis of Sclerotium rolfsii Fermented with Differential Carbon Sources

“…In our previous work, we discussed the differences in yield, rheological properties, and structures of polysaccharides under differential carbon sources [ 12 ]. Combined with the previous results on the viscosity and yield of EPS, the EPS fermented with the most popular carbon sources, sucrose and glucose, was selected as the goal of the multi-omics research.…”

“…In recent years, the formation mechanism, the effects of substrates on the yield and properties at the gene level, and the changes of metabolites are not very clear. Several bacterial polysaccharides have been reported and characterized, such as glucan [ 12 , 13 ], and fructan [ 14 ] homopolysaccharides, and hyaluronic acid [ 15 , 16 ] and xanthan gum [ 17 ] heteropolysaccharides. To summarize, the main steps of the anabolic pathways of different polysaccharides are as follows: sugar monomer absorption, glycosyl transfer, sugar nucleotide donor formation, synthesis, modification, polymerization, and the macromolecular transport of polysaccharides [ 18 ].…”