Inhibitory regulation of purple sweet potato polysaccharide on the hepatotoxicity of tri-(2,3-dibromopropyl) isocyanate

Zheng, Qi; Le, Zhiwei; Han, Furui; Feng, Yajie; Yang, Ming; Ji, Chenfeng; Zhao, Lingling

doi:10.1016/j.ijbiomac.2021.11.086

Cited by 6 publications

(4 citation statements)

References 37 publications

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“…Szychowski et al (2021) also described that, in sensitive SH‐SY5Y cells differentiated for 14 days, TBC in a wide range of concentrations (1 nM to 100 μM) produced an increase in caspase‐3 activity and ROS production (Szychowski et al, 2021). As shown by Qi et al (2022), TBC in the Hep‐G2 cell line acted via the mitochondrial pathway and involvement of death receptors (Qi et al, 2022).…”

Section: Discussionmentioning

confidence: 93%

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Involvement of peroxisome proliferator‐activated receptor gamma (PPARγ) and autophagic pathways in the mechanism of action of the tris(2,3‐dibromopropyl) isocyanurate (TDBP‐TAZTO or TBC) flame retardant in the lung adenocarcinoma (A549) cells in vitro

Szychowski

Skóra

2023

J of Applied Toxicology

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Tris(2,3‐dibromopropyl) isocyanurate (TDBP‐TAZTO or TBC) belongs to the class of novel brominated flame retardants. TBC is relatively easily released from products both during production and use; hence, it has been detected in various environmental samples. It has also been reported that TBC causes toxic effects in different cell types and now its mechanism of action is connected with oxidative stress. However, the molecular mechanism of the TBC action is mostly unknown. The aim of this study was to determine the involvement of the PPARγ receptor and certain autophagic proteins (mTOR and p62) in the mechanism of the TBC action in adenocarcinomic human alveolar basal epithelial cells (A549) in vitro. Our study showed that TBC induced toxicity only at the highest micromolar concentrations (10, 50, and 100 μM) in human A549 cells, which are a well‐established model of the alveolar type II pulmonary epithelium. TBC probably induced apoptosis only at the 50‐ and 100‐μM concentrations. However, in our experimental model, TBC showed the ability to trigger oxidative stress and affected the mRNA expression of antioxidant enzymes (SOD1 and CAT) at the lower concentrations (1 and 10 μM) than in the case of apoptosis, suggesting that the apoptosis was ROS independent. Our experiments with the PPARγ agonist (rosiglitazone) and antagonist (GW9662) suggests that TBC acted in the A549 cell line probably through activation of the mTOR‐PPARγ pathway and could interfere with the p62 autophagy pathway.

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Section: Discussionmentioning

confidence: 93%

“…(1 nM to 100 μM) produced an increase in caspase-3 activity and ROS production (Szychowski et al, 2021). As shown by Qi et al (2022), TBC in the Hep-G2 cell line acted via the mitochondrial pathway and involvement of death receptors (Qi et al, 2022).…”

Section: Discussionmentioning

confidence: 95%

Involvement of peroxisome proliferator‐activated receptor gamma (PPARγ) and autophagic pathways in the mechanism of action of the tris(2,3‐dibromopropyl) isocyanurate (TDBP‐TAZTO or TBC) flame retardant in the lung adenocarcinoma (A549) cells in vitro

Szychowski

Skóra

2023

J of Applied Toxicology

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“…Qi et al . described that TBC acts in the Hep-G2 cell line via the mitochondrial pathway and involvement of death receptors [ 18 ]. This indicates that TBC induces apoptosis in a ROS-dependent manner with engagement of the p53 protein.…”

Section: Discussionmentioning

confidence: 99%

Tris(2,3-dibromopropyl)Isocyanurate has an Effect on Inflammation Markers in Mouse Primary Astrocytes In vitro

Szychowski

Skóra

2023

Inflammation

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Abstract—Tris(2,3-dibromopropyl)isocyanurate (TBC or TDBP-TAZTO) is a new brominated flame retardant (BFR) used as a replacement of classic BFR, such as tetrabromobisphenol A. TBC is supposed to be safer than classic BFRs, but reports show that it may induce a similar toxic effect. Therefore, the aim of the present study was to determine the impact of TBC on the inflammation and activation of the apoptosis process in mouse cortical astrocytes in vitro. Our results have shown that TBC increases caspase-1 and caspase-3 activity in mouse astrocytes in vitro, which suggests inflammation-induced apoptosis. Further analyses have revealed that TBC indeed increases the level of inflammation markers, e.g. Cat, IL-1β and IL-1βR1 proteins, but decreases the level of proliferation marker protein Ki67. However, our study has demonstrated that TBC does not change the morphology of astrocytes and does not increase the number of apoptotic bodies - a well-established marker of late apoptosis. Moreover, the concentration of 50 µM TBC also increases caspase-3 activity with no formation of apoptotic bodies. However, since 10 and 50 µM TBC have never been detected in living organisms, we can assume that the compound is safe at the low concentrations that are detected.

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“…Purple sweet potatoes (Ipomoea batatas (L.) Lam), a special variety of sweet potato, are a kind of sweet potato with a purple root color. 19 The polysaccharides of the purple sweet potato (Ipomoea batatas (L.) Lam) have various biological activities, such as antioxidant, 20 antitumor, 21,22 immunomodulatory, 23 anti-inflammatory, 24,25 liver protection [26][27][28] and regulation of the intestinal flora. 29 At present, related research on whether the polysaccharides of purple sweet potato alleviate gastric ulcers and the mechanism is still to be reported.…”

Section: Introductionmentioning

confidence: 99%

Gastroprotective effects of polysaccharides from purple sweet potato (Ipomoea batatas (L.) Lam) on an ethanol-induced gastric ulcer via regulating immunity and activating the PI3K/Akt/Rheb/mTOR pathway

Sun,

Feng,

Zhang

et al. 2024

Food Funct.

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Inhibitory regulation of purple sweet potato polysaccharide on the hepatotoxicity of tri-(2,3-dibromopropyl) isocyanate

Cited by 6 publications

References 37 publications

Involvement of peroxisome proliferator‐activated receptor gamma (PPARγ) and autophagic pathways in the mechanism of action of the tris(2,3‐dibromopropyl) isocyanurate (TDBP‐TAZTO or TBC) flame retardant in the lung adenocarcinoma (A549) cells in vitro

Involvement of peroxisome proliferator‐activated receptor gamma (PPARγ) and autophagic pathways in the mechanism of action of the tris(2,3‐dibromopropyl) isocyanurate (TDBP‐TAZTO or TBC) flame retardant in the lung adenocarcinoma (A549) cells in vitro

Tris(2,3-dibromopropyl)Isocyanurate has an Effect on Inflammation Markers in Mouse Primary Astrocytes In vitro

Gastroprotective effects of polysaccharides from purple sweet potato (Ipomoea batatas (L.) Lam) on an ethanol-induced gastric ulcer via regulating immunity and activating the PI3K/Akt/Rheb/mTOR pathway

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