Inhibitory Regulation by microRNAs and Circular RNAs

Demongeot, Jacques; Hazgui, Hana; Escoffier, Jessica; Arnoult, Christophe

doi:10.1007/978-3-319-00846-2_309

Cited by 3 publications

(3 citation statements)

References 23 publications

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“…An example of the existence of accumulation of small RNA fragments exists in Sclerotinia sclerotiorum infected with the SsHV2-L virus, and these small RNAs derived from the virus measure approximately 22 nt, the same length as the miRs, suggesting a cleavage by a protein Dicer-like [ 51 ]. Regarding SARS-CoV-2, such an influence on protein translation has already been described [ 53 , 54 , 55 , 56 , 57 , 58 , 59 , 60 , 61 , 62 , 63 , 64 , 65 ], causing observed effects on the concentration of certain proteins, such as a dramatic decrease in hemoglobin [ 66 , 67 , 68 , 69 ]. If we assume that short RNA subsequences (about 20 nucleotides long), from the genes of the SARS-CoV-2 virus, can bind to Argonaut proteins and hybridize the mRNA of key human proteins, involved in metabolisms important as oxygen metabolism, it follows that recombinations, mutations and/or deletions observed in the SARS-CoV-2 genome (such as those which appeared in the United Kingdom, South Africa, France, etc., or spontaneously in vitro [ 70 , 71 , 72 , 73 , 74 , 75 , 76 , 77 ]) can reinforce the possible existence of RNA fragments, possibly capable of hybridizing for example the mRNA of subunits of hemoglobin, the gamma-globin ( Figure 6 ) and the beta-globin ( Figure 7 ), impacting the oxygen transport in infected patients.…”

Section: Resultsmentioning

confidence: 99%

mRNA COVID-19 Vaccines—Facts and Hypotheses on Fragmentation and Encapsulation

Demongeot

Fougère

2022

Vaccines

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Background: The adventure of the mRNA vaccine began thirty years ago in the context of influenza. This consisted in encapsulating the mRNA coding for a viral protein in a lipid particle. We show how the mRNA encoding S protein has been modified for that purpose in the context of the anti-SARS-CoV-2 vaccination. Results: by using data coming from genetic and epidemiologic databases, we show the theoretical possibility of fragmentation of this mRNA into small RNA sequences capable of inhibiting important bio-syntheses such as the production of beta-globin. Discussion: we discuss two aspects related to mRNA vaccine: (i) the plausibility of mRNA fragmentation, and (ii) the role of liposomal nanoparticles (LNPs) used in the vaccine and their impact on mRNA biodistribution. Conclusion: we insist on the need to develop lipid nanoparticles allowing personalized administration of vaccines and avoiding adverse effects due to mRNA fragmentation and inefficient biodistribution. Hence, we recommend (i) adapting the mRNA of vaccines to the least mutated virus proteins and (ii) personalizing its administration to the categories of chronic patients at risk most likely to suffer from adverse effects.

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Section: Resultsmentioning

confidence: 99%

mRNA COVID-19 Vaccines—Facts and Hypotheses on Fragmentation and Encapsulation

Demongeot

Fougère

2022

Vaccines

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“…Moreover, RNA viruses can also form complexes with existing mRNAs and/or proteins of host cells. Thereby they might prevent protein function, behave like microRNAs [3] , [4] , [5] , [6] or ribosomal RNAs [6] , [7] , [8] , inhibiting or favoring the translation of specific proteins of host cells [9] , [10] , [11] , [12] , [13] , [14] , [15] , [16] , [17] . If these proteins are vital for the host, viral pathogenicity is much greater than that caused by viral replication.…”

Section: Introductionmentioning

confidence: 99%

“…Thereby they might prevent protein function, behave like microRNAs [3][4][5][6] or ribosomal RNAs [6][7][8],…”

Section: Introductionmentioning

confidence: 99%

SARS-CoV-2 and miRNA-like inhibition power

Demongeot

Seligmann

2020

Medical Hypotheses

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(1) Background: RNA viruses and especially coronaviruses could act inside host cells not only by building their own proteins, but also by perturbing the cell metabolism. We show the possibility of miRNA-like inhibitions by the SARS-CoV-2 concerning for example the hemoglobin and type I interferons syntheses, hence highly perturbing oxygen distribution in vital organs and immune response as described by clinicians ; (2) Hypothesis: We hypothesize that short RNA sequences (about 20 nucleotides in length) from the SARS-CoV-2 virus genome can inhibit the translation of human proteins involved in oxygen metabolism, olfactory perception and immune system. (3) Methods: We compare RNA subsequences of SARS-CoV-2 protein S and RNA-dependent RNA polymerase genes to mRNA sequences of beta-globin and type I interferons; (4) Results: RNA subsequences longer than eight nucleotides from SARS-CoV-2 genome could hybridize subsequences of the mRNA of beta-globin and of type I interferons; (5) Conclusions: Beyond viral protein production, COVID-19 might affect vital processes like host oxygen transport and immune response.

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