Inhibitory properties of some heavy metals on carbonic anhydrase I and II isozymes activities purified from Van Lake fish (Chalcalburnus Tarichi) gill

Kuzu, Müslüm; Çomaklı, Veysel; Akkemik, Ebru; Çiftçi, Mehmet; Küfrevioğlu, Ömer İrfan

doi:10.1007/s10695-018-0499-8

Cited by 6 publications

(4 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The% activity‐[extract] graph was drawn using the obtained data. IC 50 values were calculated using the graph [59] …”

Section: Methodsmentioning

confidence: 99%

Enzyme Inhibition and Antioxidant Activities of Asparagus officinalis L. and Analysis of Its Phytochemical Content by LC/MS/MS

Çomaklı¹,

Kaya²,

Kuzu³

et al. 2023

Chemistry & Biodiversity

Self Cite

View full text Add to dashboard Cite

In the study, water, ethanol, methanol, dichloromethane, and acetone extracts of Asparagus officinalis L. were obtained by maceration. DPPH⋅, ABTS⋅+, FRAP, and CUPRAC methods determined the antioxidant capacities of all extracts. Moreover, the in vitro effects of extracts on acetylcholinesterase (AChE), butyrylcholinesterase (BChE), carbonic anhydrase (CA)‐I, CA‐II and α‐Glycosidase were investigated. At a 10 μg/ml concentration, the extract with the highest Fe3+ reduction capacity was ethanol (AE), and the extract with the highest Cu2+ reduction capacity was acetone (AA). AE for AChE (IC50=21.19 μg/ml) and α‐Glycosidase (IC50: 70.00 μg/ml), methanol (AM) for BChE (IC50=17.33 μg/ml), CA−I and II (IC50=79.65 and 36.09 μg/ml, respectively) showed the most potent inhibition effect. The content analysis of acetone extract was performed with LC/MS‐MS, the first three phytochemicals found most were p‐Coumaric acid, rutin, and 4‐hydroxybenzoic acid (284.29±3.97, 135.39±8.19, and 102.06±5.51 μg analyte/g extract, respectively).

show abstract

“…The% activity‐[extract] graph was drawn using the obtained data. IC 50 values were calculated using the graph [59] …”

Section: Methodsmentioning

confidence: 99%

Enzyme Inhibition and Antioxidant Activities of Asparagus officinalis L. and Analysis of Its Phytochemical Content by LC/MS/MS

Çomaklı¹,

Kaya²,

Kuzu³

et al. 2023

Chemistry & Biodiversity

Self Cite

View full text Add to dashboard Cite

show abstract

“…The obtained supernatant was applied to a Sepharose 4B-L-tyrosine-sulfanilamide affinity column in accordance with a previously described procedure, and the CA-I and CA-II enzymes were separately eluted. 39 The esterase activity of the CA enzyme was used in enzyme activity measurements. The activity measurements were performed spectrophotometrically at 348 nm according to the method described by Verpoorte and colleagues.…”

Section: Purification Of Carbonic Anhydrase and Kinetic Studiesmentioning

confidence: 99%

Microvawe assisted synthesis of N-(methyl and methoxy) benzylidene-4-fluoroaniline derivatives and their carbonic anhydrase I and II inhibition properties

Çelik¹,

Kuzu²

2019

Org.Commun.

Self Cite

View full text Add to dashboard Cite

In this study, some Schiff base derivatives (6a-f) were synthesized using a microwave method. The synthesized compounds were characterized by 1D, 2D NMR and mass spectral data. Their inhibitory effects were studied on carbonic anhydrase isozymes (hCA I and II), purified from human erythrocyte cells by Sepharose-4B-Ltyrosine-sulfanilamide affinity chromatography and the compounds N-3-Methylbenzylidene-4-fluoroaniline (6b), N-4-Methylbenzylidene-4-fluoroaniline (6c), N-2-Methoxybenzylidene-4-fluoroaniline (6d)showed moderate activity on hCAII in the range of IC50 values.

show abstract

“…Their high presence in the environment is due to anthropogenic activities, including the application of paint, batteries, metal scraps, motor oil, pesticideherbicides, and fertilizers (Awokunmi, 2010). The development in industry, agriculture, and mining and the increase in their activity has led to increased heavy metal pollution (Kalay and Yasam 2000;Kuzu et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

Investigation of Heavy Metal Concentrations and Accumulation Capacities of Naturally Growing Species in Old Garbage Area

İnci,

Ahıskalı,

Macit

et al. 2024

Yüzüncü Yıl Üniversitesi Tarım Bilimleri Dergisi

View full text Add to dashboard Cite

In underdeveloped and/or developing countries, garbage is often randomly piled up in open areas. This method has been used to dispose of garbage/solid waste in Turkey for many years. Although pollution is not at the forefront in Bingöl province, the area located in the city center of the city has been used as a wild garbage storage area for approximately 18 years. Since the garbage in the area poses a danger to people and the environment, this area has become inactive with the establishment of a new solid waste disposal facility in the city. There are plants that have adapted to this area, which has been empty for about ten years. In this study, it was tried to determine in what proportions and organs the plant species distributed in the area accumulate heavy metals that may have come from garbage leachate. Plants identified in the field; Alyssum simplex, Cirsium libanoticum, Descurainia sophia, Fumaria asepala, Fumaria officinalis, Matricaria chamomilla, Papaver dubium, Scrophularia canina, Trifolium repens and Ziziphora capitata species. Fe, Cr, As, Cd and Pb concentrations (mg kg-1) of these species were measured in root, stem, leaf and flower organs and translocation factors (TF) were calculated for these species. In conclusion; Alyssum simplex, Cirsium libanoticum and Fumaria asepala for Fe, Cirsium libanoticum, Fumaria asepala, Fumaria officinalis and Matricaria chamomilla Cr and As, Cirsium libanoticum, Papaver dubium and Scrophularia canina for Cd and all other species except Alyssum simplex and Scrophularia canina for Pb translocation factors (TF) were found to be greater than 1 (TF>1). The accumulation potential of these species is thought to be promising so that they can be evaluated in phytoremediation.

show abstract

Inhibitory properties of some heavy metals on carbonic anhydrase I and II isozymes activities purified from Van Lake fish (Chalcalburnus Tarichi) gill

Cited by 6 publications

References 35 publications

Enzyme Inhibition and Antioxidant Activities of Asparagus officinalis L. and Analysis of Its Phytochemical Content by LC/MS/MS

Enzyme Inhibition and Antioxidant Activities of Asparagus officinalis L. and Analysis of Its Phytochemical Content by LC/MS/MS

Microvawe assisted synthesis of N-(methyl and methoxy) benzylidene-4-fluoroaniline derivatives and their carbonic anhydrase I and II inhibition properties

Investigation of Heavy Metal Concentrations and Accumulation Capacities of Naturally Growing Species in Old Garbage Area

Contact Info

Product

Resources

About