Enzyme Inhibition and Antioxidant Activities of Asparagus officinalis L. and Analysis of Its Phytochemical Content by LC/MS/MS

Abstract

In the study, water, ethanol, methanol, dichloromethane, and acetone extracts of Asparagus officinalis L. were obtained by maceration. DPPH⋅, ABTS⋅+, FRAP, and CUPRAC methods determined the antioxidant capacities of all extracts. Moreover, the in vitro effects of extracts on acetylcholinesterase (AChE), butyrylcholinesterase (BChE), carbonic anhydrase (CA)‐I, CA‐II and α‐Glycosidase were investigated. At a 10 μg/ml concentration, the extract with the highest Fe3+ reduction capacity was ethanol (AE), and the extract with the highest Cu2+ reduction capacity was acetone (AA). AE for AChE (IC50=21.19 μg/ml) and α‐Glycosidase (IC50: 70.00 μg/ml), methanol (AM) for BChE (IC50=17.33 μg/ml), CA−I and II (IC50=79.65 and 36.09 μg/ml, respectively) showed the most potent inhibition effect. The content analysis of acetone extract was performed with LC/MS‐MS, the first three phytochemicals found most were p‐Coumaric acid, rutin, and 4‐hydroxybenzoic acid (284.29±3.97, 135.39±8.19, and 102.06±5.51 μg analyte/g extract, respectively).

A Review of the Pro-Health Activity of Asparagus officinalis L. and Its Components

“…They note that the extraction yield is significantly influenced by solvent composition, temperature and extraction time and propose that that optimal extraction occurs using 50% ethanol as a solvent with a liquid-solid ratio of 30:1, extraction temperature of 80 • C, and time of two hours. Phytochemical analysis using liquid chromatography/mass spectrometry (LC/MS)-MS by Comakli et al [12] identified three phytochemicals in acetone extract from the aerial parts of A. officinlis: p-coumaric acid, rutin, and 4-hydroxybenzoic acid (284.3 ± 4.0; 135.4 ± 8.2, and 102.1 ± 5.5 µg analyte/g extract, respectively).…”

“…Recently, the antioxidant activities of water, methanol, ethanol, chloroform, and acetone extracts of aerial parts of A. officinalis have been studied using various methods, including ABTS, DPPH, ferric ion reducing antioxidant power (FRAP), and Cu 2+ ion reduc-tion (CUPRAC). Comakli et al [12] found the ethanol extract (10 µg/mL) to demonstrate the highest Fe 3+ reduction capacity, and acetone extract (10 µg/mL) displayed the greatest Cu 2+ reduction potential. Moreover, all tested extracts appeared to inhibit the activity of the enzymes acetylcholinesterase, butyrylcholinesterase, carbolic anhydrase and α-glucosidase, which are involved in various diseases, in vitro; it was also found that consumption of A. officinalis can support the treatment of certain diseases, including glaucoma, epilepsy, Alzheimer's disease, and type 2 diabetes.…”

“…Anti-diabetic (in vivo-rat model) [33] Antioxidant property (in vivo-rat model) [31] Antinociceptive property (in vivo-rat model) [31] Hypolipidemic property (in vivo-mouse model) [36,37] Spears Antioxidant property (in vitro) [12] Anti-cancer property (in vitro) [38] Antifungal property (in vitro) [27,39,42] Antimicrobial property (in vitro) [27,42] Shoots Antioxidant property (in vivo mouse model) [32] Leaves Antioxidant property (in vivo mouse model) [32] Seeds Anti-diabetic (in vivo-rat model) [34,35]…”