Inhibitory Power Test of Two Trichoderma Isolates in In Vitro Way Againts Fusarium oxysporum The Cause of Red Chilli Stem Rot

2021

E3S Web Conf.

This study aims to determine the effect of bacterial isolates of Bacillus subtilis Bs-Sdj-01 and Trichoderma sp. Tc-Jro-02 isolates as biological fertilizer in plant growth until the end of the vegetative phase. The experiment was arranged factorially in a Completely Randomized Design (CRD) with each factor being the application of B. subtilis consisting of and without B. subtilis and the Trichoderma application consisting of with and without Trichoderma; the experiment was repeated four times. The observed variables were: plant height, stem diameter, number of leaves, leaf area, stover dry weight, root dry weight, and B. subtilis population at the end of the observation. Data were analyzed using with ANOVA and HSD tests at the 5% level. The combination of Trichoderma sp. and B. subtilis bacteria produce the highest increase in plant height, stem diameter, number of leaves, leaf area, stover dry weight, and root dry weight of soybean plants up to 35 days after planting and increase B. subtilis population grew from 1010 CFU.g-1 to be 4.43x1011 CFU.g-1. The presence of Trichoderma supports the activity of B. subtilis in the rhizosphere of the Detam variety soybean plantation.

Section: Discussionmentioning

confidence: 99%

Section: Preparation and Plantingmentioning

confidence: 99%

The Vegetative Growth Response of Detam Soybean Varieties towardsBacillus subtilisandTrichodermasp. Applications as Bio-fertilizer

Miftakhurrohmat

2021

E3S Web Conf.

“…Pc-Sdj-07, meanwhile, the inhibition of two Trichoderma isolates was very weak (Figure 4). The fungus T. asperellum which, prior to species determination based on molecular markers [21], based on morphological characteristics was considered as T. harzianum, was effective in controlling the fungus R. solani pathogen damping-off on soybean [22], F. oxysporum causes root powder of red chili [23], Phytophthora infestans pathogen late blight on potatoes [24], and Colletotrichum spp. anthracnose pathogen in chili [25], and.…”

Section: Inhibition Of T Asperellum Against Candidate Fungi Of Biofer...mentioning

confidence: 99%

Fungistatic Effect of Ipomea Carnea Extract and Trichoderma Esperellum Against Various Fungal Biological Agents

IOP Conf. Ser.: Earth Environ. Sci.

Prihatiningrum

Miftahurrohmat

2022

The study aimed to measure the fungistatic effect of Ipomea carnea and Trichoderma esperellum Tc-Jjr-02 biomass extracts against several fungal isolates of biological agents in vitro. Nine selected biological agent fungi were grown in PDA-chloramphenicol media containing 1% fresh extract of I. carnea biomass, four of them were grown in dual culture with isolate Tc-Jjr-02 with an incubation period of 96 hours. The results showed that the I. carnea biomass extract had a fungistatic effect by inhibiting the growth of the entomopathogenic agents Metarrhizium anipsoliae Me-Sdj-16 (27.8±3.2%) and Beuveria bassiana Be-Sj-13 (38.1±2.6%) and Be-Sdj-15 (18.6±1.0%), and the biofertilizer agents Aspergillus sp. As-Sdj-11 (46.7±1.9%), Pénicillium sp. Pc-Sdj-14 42.7±3.5%, and Trichoderma sp. Tc-Sdj-18 38.5±1.0% at 96 hours incubation period (HAI). The biocontrol agent T. esperellum gave a weak fungistatic effect against the biofertilizer agents As-Sdj-11 and Pc-Sdj-07 were 14.4±1.3% and 7.0±1.9% at 96 HAI and did not have a fungistatic effect on the biofertilizer agents Trichoderma Tc-Ba-05 and Tc-Sdj-09. Application of I. carnea biomass as green manure and some biofertilizer agents can be carried out simultaneously; while the application of T. esperellum can be carried out using biofertilizer agents As-Sdj-11 and Pc-Sdj-07 and Tc-Ba-05 and Tc-Sdj-09.

“…Trichoderma adalah salah satu genus fungi yang memberikan prospek besar bagi implementasi pertanian ramah lingkungan terutama efek aktivitasnya sebagai penyedia nutrisi bagi tanaman dan agensia yang mendukung pertumbuhan tanaman. Trichoderma adalah fungi yang bisa digunakan sebagai pengendali hayati di samping memiliki kemampuan sebagai agensia biofertilasi bagai tanaman (Wachid & Sutarman, 2019). Fungi ini di samping menghasilkan senyawa antimetabolit yang dapat menghambat patogen sekaligus mampu mendegradasi bahan organik yang menghasilkan nurisi bagi tanaman (Li et al, 2019;Sutarman, 2019).…”

Section: Pendahuluanunclassified

Uji Keragaan Trichoderma Sebagai Pupuk Hayati Dalam Meningkatkan Pertumbuhan Dan Produksi Tanaman Bawang Merah

Prahasti

2022

JAT

Penelitian ini bertujuan untuk membuktikan pengaruh Trichoderma isolate Tc-Pct-05 yang diformulasi dalam campuran tepung sekam-kulit singkong sebagai pupuk hayati meningkakan pertumbuhan dan produksi tanaman bawang merah. Penelitian ini dilaksanakan di lahan pertanian Dusun Sumberan Desa Sajen Kecamatan Pacet Kabupaten Mojokerto pada Desember 2021-Maret 2022. Kegiatan formulasi agen hayati biofertilasi dilaksanakan di Laboratorium Mikrobiologi Universitas Muhammadiyah Sidoarjo. Percobaan disusun dalam Rancangan Acak Lengkap dengan empat perlakuan cara aplikasi yaitu soil treatment, apical treatment, soil-apical treatment, dan control, masing-masing dengan lima ulangan, sehingga secara keseluruhan terdapat 20 satuan percobaan. Variabel yang diamati meliputi tinggi tanaman, bobot umbi, jumlah daun, dan berat brangkasan. Semua data dianalisis dengan menggunakan Analisis Ragam (ANOVA) yang dilanjutkan dengan uji Duncan pada taraf uji 5% untuk mengetahui perbedaan antar perlakuan. Hasil penelitian menunjukkan bahwa pupuk hayati Trichoderma yang diaplikasikan sebagai soil treatment dengan dosis per tanaman 250 gram mampu meningkatkan tinggi tanaman, jumlah daun, bobot basah dan bobot kering brangkasan tanaman, serta meningkatkan bobot basah umbi bawang merah 145% dibandingkan perlakuan secara konvensional menggunakan pupuk kimia sepenuhnya.