Inhibitory Effects of Vanadium-Binding Proteins Purified from the Sea Squirt Halocynthia roretzi on Adipogenesis in 3T3-L1 Adipocytes

Gunasinghe, Minoli Anuththara; Kim, Aaron Taehwan; Kim, Sang Moo

doi:10.1007/s12010-019-02982-7

Cited by 7 publications

(11 citation statements)

References 40 publications

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“…However, the cells treated with 40 μg/mL of V-P complex displayed a significant time-dependent reduction, the maximum inhibitory effect (17.25%) was observed at day 14 ( Fig 1 ). These indicated that V-P complex with concentration less than 20 μg/mL had low cytotoxicity, which were in agreement with literature [ 23 ], in which VBP from sea squirt had low cell toxicity after differentiation in 3T3-L1 for 6 days. It has been demonstrated that organic vanadium compounds didn’t cause any gastro-intestinal discomfort or liver and kidney toxicity [ 29 ].…”

Section: Resultssupporting

confidence: 92%

“…The reduction of lipid content observed in this work and previous studies might be attributed to the existence of vanadium in vanadium-protein compounds [10,12]. These results were supported by Gunasinghe and Kim [23], in which the inorganic vanadium and VBP decreased the lipid content in 3T3-L1 differentiated cells at day 6. It has been demonstrated that lipid content and the extent of body fat accumulation are tightly associated with the occurrence and development of obesity [30].…”

Section: Effect Of V-p Complex On Lipid Accumulationsupporting

confidence: 90%

“…in 3T3-L1 mature cells at day 6 [23]. Additionally, previous studies have reported that male B6�BKS (D) db/db mice were treated with synthetic vanadyl complexes, the protein expression of PPARα/γ in adipose tissues was increased, revealed that vanadium compounds had potent insulin enhancement effect [37,38].…”

Section: Plos Onementioning

confidence: 89%

“…Furthermore, the recent investigation showed that the Wnt signaling palyed an important role in metabolism and adipocyte biology in obesity and type 2 diabetes [9]. The previous study also suggested that the natural vanadium compound VBP inhibited 3T3-L1 preadipocyte differentiation through activation of Wnt/β-catenin pathway [12,23].…”

Section: Effect Of V-p Complex On the Expression Of Adipocyte Specifimentioning

confidence: 95%

“…Organic vanadium compounds such as vanadium-flavonol complex could reduce blood glucose in STZ-induced rats [ 2 ]. Natural vanadium compounds such as vanadium-binding protein (VBP) extracted from sea cucumber or sea squirt could inhibit adipocyte differentiation in 3T3-L1 cells [ 12 , 23 ]. Additionally, our previous study showed that the synthetic vanadium-protein complex had antidiabetic activity in vitro [ 24 ].…”

Section: Introductionmentioning

confidence: 99%

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Vanadium-protein complex inhibits human adipocyte differentiation through the activation of β-catenin and LKB1/AMPK signaling pathway

Zhang

Liu²,

Kim

2020

PLoS ONE

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Obesity is a common disease over the world and is tightly associated with diabetes mellitus, cardiovascular and cancer disease. Although our previous study showed that the synthetic vanadium-protein (V-P) complex had a better effect on antioxidant and antidiabetic, the relative molecular mechanisms are still entirely unknown. Hence, we investigated the effect of the synthetic V-P complex on adipocyte differentiation (adipogenesis) using human preadipocytes to clarify its molecular mechanisms of action. The primary human preadipocytes were cultured with and without V-P complex during adipocyte differentiation. The cell proliferation, lipid accumulation, and the protein expression of transcription factors and related enzymes were determined for the differentiated human preadipocytes. In this study, the 20 μg/mL of V-P complex reduced the lipid and triglyceride (TG) content by 74.47 and 57.39% (p < 0.05), respectively, and down-regulated the protein expressions of peroxisome proliferator-activated receptor-γ (PPARγ), CCAAT/enhancer-binding protein alpha (C/ EBPα), sterol regulatory element-binding protein 1 (SREBP-1) and fatty acid synthase (FAS). Additionally, the V-P complex significantly up-regulated the protein levels of total βcatenin (t-β-catenin), nuclear β-catenin (n-β-catenin), phosphorylated adenosine monophosphate-activated protein kinase alpha (p-AMPKα) and liver kinase B1 (p-LKB1). These showed that the inhibitory effect of V-P complex on human adipogenesis was mediated by activating Wnt/β-catenin and LKB1/AMPK-dependent signaling pathway. Therefore, the synthetic V-P complex could be considered as a candidate for prevention and treatment of obesity.

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Section: Resultssupporting

confidence: 92%