Inhibitory effects of triterpenes isolated from Hoelen on free radical‐induced lysis of red blood cells

Abstract

Hoelen, sclederma of Poria cocos Wolf, has long been used as a sedative and diuretic in traditional medicine. Formerly, we demonstrated that Hoelen in vitro protects red blood cells from AAPH-induced hemolysis. In this study, tests were carried out to identify the main ingredient of Hoelen that has the scavenging effect on free-radicals. Triterpene carboxylic acids isolated from the methanol extract of Hoelen, i.e. pachymic acid, polyporenic acid, 3-epidehydrotumulosic acid, 3beta-hydroxylanosta-7,9(11), 24-trien-21-oic acid and 3-o-acetyl-16 alpha -hydroxytrametenolic acid, were found to have inhibitory activities against AAPH-induced lysis of red blood cells.

Cytotoxic and Anti-oxidant Activities of Lanostane-Type Triterpenes Isolated from Poria cocos

“…None of the compounds was powerful scavenger of DPPH free radicals (data not shown). These results contradict those reported by Sekiya et al, 21) who found that lanostane-type triterpenes from P. cocos had inhibitory activities against AAPH [2,2Ј-azobis(2-amidinopropane)dihydrochloride]-induced lysis of red blood cells. Sekiya et al found that incubation of red blood cells with triterpenes and APPH, a peroxyl radical generator, inhibited cell lysis and reduced residual APPH.…”

“…Lanostane-type triterpenes were previously implicated to possess antioxidant and free radical scavenging activities. 21) To determine if our compounds possess any antioxidant and free radical scavenging activity, we studied their ability to scavenge DPPH free radicals. None of the compounds was powerful scavenger of DPPH free radicals (data not shown).…”

Advanced ultra-performance liquid chromatography–photodiode array–quadrupole time-of-flight mass spectrometric methods for simultaneous screening and quantification of triterpenoids in Poria cocos

“…The main chemical constituents of P. cocos are polysaccharides, triterpenoids, egosterol and proteins (Tai, Akahori, & Shingu, 1992;Tai, Shingu, Kikuchi, Tezuka, & Akahori, 1995a;Tai, Shingu, Kikuchi, Tezuka, & Akahori, 1995b;Wang & Wan, 1998;Yang & Bao, 2005), in which triterpenoids are found to possess interesting pharmacological actions such as cytotoxic and anti-oxidant (Zhou et al, 2008), anti-cancer (Gapter, Wang, Glinski, & Ng, 2005), anti-inflammatory (Yasukawa et al, 1998), inhibition of free radical-induced lysis of red blood cells (Sekiya, Goto, Shimada, & Terasawa, 1998) and inhibition of calf DNA polymerase a and rat DNA polymerase b (Akihisa et al, 2004;Kaminaga et al, 1996). Up to now, more than 80 triterpenoid compounds were isolated and identified from P. cocos with the above-mentioned bioactivities.…”

Neuroprotection by herbal formula FBD and its active compounds

“…On one hand, various biological activities of the six compounds were implicated in the neuroprotections of their combination or FBD-CO 2 . Antiplatelet activity of ferulic acid (Wang et al, 1988), and anti-inflammatory activities of atractylenolide I, III and pachymic acid could contribute to the effects of their combination or FBD-CO 2 on thrombus or inflammationinduced neuronal injury (Giner Larza et al, 2000;Li et al, 2007;Sekiya et al, 2003). On the other hand, some other active compounds from FBD might further be additive to the combination.…”

“…Up to now, few psychoneural activities were available on these compounds except ferulic acid. As phospholipase A2 inhibitor (Cuella et al, 1996;, and DNA topoisomerases inhibitor (Li et al, 2004), pachymic acid from Fu Ling inhibited tumor (Gapter et al, 2005;Kaminaga et al, 1996), and free-radical-induced hemolysis (Sekiya et al, 2003). Of the three sesquiterpenoids from Bai Zhu, atractylenolide I and III inhibited pro-inflammatory cytokines production and uterine contraction Zhang et al, 2000).…”