Abstract:The objective of this study was to investigate the inhibitory effects of tanshinone OOA on human bladder cancer BOU-87 cells and the xenograft in nude mice. BOU-87 cells were treated with tanshinone OOA with concentration of 0.5, 1, 2 and 4 g/L. The cell proliferation, cycle and apoptosis were detected. The nude mice with BOU-87 cell xenograft were treated with normal saline (control), 5-fluorouracil (5-FU), 200, 400 and 600 mg/kg tanshinone OOA, respectively. The animal body weight and growth of tumor were me… Show more
“…Studies have found that many plants contain the ingredients which have good activities in antioxidant, anti-inflammatory, anti-tumor and other aspects (Costa et al, 2020;Huang et al, 2020;Santos et al, 2021). Curcumol is a sesquiterpene compound isolated from the zedoary turmeric oil which has long been used as a folk medicine in China for many years (You et al, 2005).…”
This study investigated the inhibitory effect and mechanism of curcumol on growth of human hepatocellular carcinoma (HCC) HepG2 xenografts in nude mice. Curcumol was isolated from from zedoary turmeric oil. The HepG2 xenograft model in nude mice was established. Forty modeled nude mice were divided into model, cisplatin, curcumol and cisplatin+curcumol groups. The latter three groups were treated with 2 mg/kg cisplatin, 100 mg/kg curcumol and 2 mg/kg cisplatin combined with 100 mg/kg curcumol, respectively, once every two days, for total seven times. After treatment, compared with cisplatin group, in cisplatin+curcumol group the tumor weight and tumor inhibition rate were obviously increased, the apoptosis rate of HepG2 cells was obviously increased, the expression levels of Caspase-3 and B-cell lymphoma-2 associated X proteins in xenograft tumor were significantly increased, and the expression level of B-cell lymphoma-2 protein was significantly decreased. In addition, the thymus index and spleen index of animal had no significant difference between cisplatin group and cisplatin+curcumol group. In conclusion, the additional use of curcumol can obviously increase the inhibitory effect of cisplatin on growth of HCC HepG2 xenografts in nude mice. The mechanism may be related to the enhanced promotion of apoptosis of tumor.
“…Studies have found that many plants contain the ingredients which have good activities in antioxidant, anti-inflammatory, anti-tumor and other aspects (Costa et al, 2020;Huang et al, 2020;Santos et al, 2021). Curcumol is a sesquiterpene compound isolated from the zedoary turmeric oil which has long been used as a folk medicine in China for many years (You et al, 2005).…”
This study investigated the inhibitory effect and mechanism of curcumol on growth of human hepatocellular carcinoma (HCC) HepG2 xenografts in nude mice. Curcumol was isolated from from zedoary turmeric oil. The HepG2 xenograft model in nude mice was established. Forty modeled nude mice were divided into model, cisplatin, curcumol and cisplatin+curcumol groups. The latter three groups were treated with 2 mg/kg cisplatin, 100 mg/kg curcumol and 2 mg/kg cisplatin combined with 100 mg/kg curcumol, respectively, once every two days, for total seven times. After treatment, compared with cisplatin group, in cisplatin+curcumol group the tumor weight and tumor inhibition rate were obviously increased, the apoptosis rate of HepG2 cells was obviously increased, the expression levels of Caspase-3 and B-cell lymphoma-2 associated X proteins in xenograft tumor were significantly increased, and the expression level of B-cell lymphoma-2 protein was significantly decreased. In addition, the thymus index and spleen index of animal had no significant difference between cisplatin group and cisplatin+curcumol group. In conclusion, the additional use of curcumol can obviously increase the inhibitory effect of cisplatin on growth of HCC HepG2 xenografts in nude mice. The mechanism may be related to the enhanced promotion of apoptosis of tumor.
“…Tanshinone in S. miltiorrhiza has definite curative effect on cardiovascular diseases and neurological diseases (Hao et al, 2015;Xu et al, 2021). Recently, tanshinone has been found to have an inhibitory effect on human bladder cancer cells (Huang et al, 2020). In addition, Gengmao et al (2014) found that the adaptive response to salt stress in S. miltiorrhiza was linked with its highly induced antioxidant enzyme defense systems.…”
S. miltiorrhiza is a Chinese medicinal plant that is widely cultivated. The root growth in S. miltiorrhiza are inhibited by soil salinity. Here we investigated the capability of a plant growth regulator, 5-ALA to promote the growth of S. miltiorrhiza under different salt stresses. Five-month old S. miltiorrhiza roots were uniformly irrigated with different levels of salt solution i.e. 0, 100, 200 mM NaCl. After 3 days of treatment, salt-treated S. miltiorrhiza plants were sprayed with different concentrations of ALA (0, 10 mg L -1 , 20 mg L -1 ) on the leaves and cultured for another 7 days. Results revealed that ALA treated plants produced significantly higher biomass by sustaining leaf chlorophyll content under salt stressed. 10 mg L -1 ALA significantly up-regulated antioxidant enzymes activities under studied salinity treatments. Positive effects of ALA on antioxidant defense systems were also supported by a significant increase in the expression of SOD isoenzymes genes (CSD1, FSD1 and MSD2), defense response genes (DXS1, C4H, GGPPS) and stress-related gene (MYB36 , MYB39) of ALA treated plants. This study suggested that ALA can protect S. miltiorrhiza from salinity induced oxidative stress and injury by promoting antioxidant defense system, boosting secondary metabolic pathways and protecting photosynthetic pigments.
“…Moreover, Lachnospiraceae is a highly abundant bacterial family within the gut microbiota in human, whose members may protect against human colon cancer by producing butyric acid, a substance that is important for both microbial and host epithelial cell growth (Meehan & Beiko, 2014;Yang et al, 2020;Huang et al, 2020;Hwang et al, 2019;Grom et al, 2020). In this study, the compositions of Lachnospiraceae in the control, lowdose, medium-dose and high-dose groups were 34.1%, 36.9%, 32.84% and 31.39%, respectively.…”
We aimed to evaluate the effect of red meat diet on gut microbiota in mice. Balb/c mice at weaning were randomized into control group and red meat groups with different proportions (25%, 50%, and 75%). Mice were fed with a standard pellet diet as control group, while those were fed with different proportions of red meat diet as red meat groups. After 8 weeks, they were sacrificed and their intestinal contents were obtained for 16S rRNA sequencing and bioinformatics analysis. Our results showed that there were significant-structural differences among the four groups. The top-two most abundant phylum were Firmicutes and Bacteroidetes. In the red meat groups, the abundance of Bacteroidetes was increased, but the abundance of Firmicutes was decreased. At the family level, Bacteroidaceae and Family XIII were significantly higher in the high-dose group than those in the control group. There were also significant differences in abundance of many genera. In conclusion, different proportions of red meat diet may lead to changes in gut microbial flora in mice. These changes may be pathological and may be related to the frequent occurrence of many diseases.
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