Inhibitory effects of probiotic Bacillus coagulans against MCF7 breast cancer cells

Dolati, Masoumeh; Tafvizi, Farzaneh; Salehipour, Masoud; Movahed, Tahereh Komeili; Jafari, Parvaneh

doi:10.18502/ijm.v13i6.8089

Cited by 17 publications

(23 citation statements)

References 34 publications

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“…The activity of Bacillus sp. against various types of cancer cells were reported [64][65][66][67][68]. These findings highlight the importance of Bacillus sp.…”

Section: Discussionsupporting

confidence: 53%

Anticancer and antibacterial activities of secondary bioactive compounds from endosymbiotic bacteria of aphid and their surrounding organisms (Predator and protector)

Alsufyani

Al-Otaibi

Alotaibi

et al. 2022

Preprint

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Secondary metabolites of bacterial origin are a valuable source of diverse molecules with antibacterial and anticancer activities. In the current study, 10 endosymbiotic bacteria were isolated from aphids, aphid predators and ants. Bacterial strains were identified based on the 16S rRNA gene. Crude extracts were prepared from each isolated bacteria and tested for their antibacterial activities using the disk diffusion method. The extracts of three bacterial species;Planococcussp,Klebsiella aerogenes, Enterococcus aviumfromAphis punicae, Chrysoperia carneaandTapinoma magnum, respectively were found to have strong antibacterial activities against one or more of the five pathogenic bacteria tested. The inhibition zones ranged from 10.00± 0.13 to 20.00± 1.11mm with minimum inhibitory concentration (MIC) values ranged from 0.156 mg/mL to 1.25 mg/mL. The notable antibacterial activity was for the crude extract ofK. aerogenesagainstKlebsiella pneumoniaandEscherichia coliat MIC value of 0.156 mg/mL. The cytotoxic activity of the crude extracts varied according to the tested cell line. The most cytotoxic effect was for the extracts ofK. aerogenesandE. aviumat a concentration of 0.16 mg/mL against lung carcinoma epithelial cells (A549) with cell reduction of 79.4% and 67.2%, respectively. Crude extracts ofK. aerogenesandPantoea agglomeransat a concentration of 0.16 mg/mL showed 69.4% and 67.8% cell reduction against human colon cancer (Hct116), respectively. Gas chromatography–mass spectrometry (GC-MS) analysis of three crude extracts revealed the presence of several bioactive secondary metabolites that have been reported previously to possess antibacterial and anticancer properties. To the best of our knowledge, this is the first study to report the biological activities of endosymbiotic bacteria from aphids, aphid predators and ants. The promising data presented in this study opens a new avenue for alternative drugs to overcome the continuous emergence of multidrug-resistant bacteria and to find alternative drugs for traditional cancer therapies.

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“…The activity of Bacillus sp. against various types of cancer cells were reported [64][65][66][67][68]. These findings highlight the importance of Bacillus sp.…”

Section: Discussionsupporting

confidence: 53%

Anticancer and antibacterial activities of secondary bioactive compounds from endosymbiotic bacteria of aphid and their surrounding organisms (Predator and protector)

Alsufyani

Al-Otaibi

Alotaibi

et al. 2022

Preprint

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“…The culture of Bacillus coagulans and preparation of cell-free supernatant were carried out according to a previous study 35 . Briefly, B. coagulans (GBI-30, 6068) were bought as lyophilized from Ganeden, Inc. (the USA).…”

Section: Methodsmentioning

confidence: 99%

“…To finish the synthesis of metabolites, 10% of the overnight culture was inoculated into an MRS broth medium and cultured for 72 h. Centrifugation was carried out at 6000 rpm (Hettich, Germany), at room temperature, for 20 min to obtain the bacterial culture supernatant. Then, 100 mL of the culture medium was thickened with a freeze dryer to about 20 mL and stored in a refrigerator 35 .…”

Section: Methodsmentioning

confidence: 99%

Biogenic copper oxide nanoparticles from Bacillus coagulans induced reactive oxygen species generation and apoptotic and anti-metastatic activities in breast cancer cells

Dolati

Tafvizi

Salehipour

et al. 2023

Sci Rep

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The present study examined the anticancer capabilities of Bacillus coagulans supernatant-produced copper oxide nanoparticles (BC-CuONPs) on MCF-7 and SKBR3 cancer cells. The X-ray diffraction, ultraviolet–visible spectroscopy, Fourier-transform infrared spectroscopy, X-ray photoelectron spectroscopy, transmission electron microscopy, field-emission scanning electron microscopy, energy-dispersive X-ray, dynamic light scattering, and zeta potential techniques were used to characterize BC-CuONPs. This study also investigated the cellular and molecular processes of NPs’ anti-proliferative and apoptotic properties on human breast cancer cells and compared them to the commercial pharmaceutical tamoxifen. The size of the spherical NP was from 5 to 47 nm with negative zeta potential. The MTT results showed the great cytotoxic effect of BC-CuONPs against breast cancer cells. The BC-CuONPs inhibited the growth of breast cancer cells in a time- and dose-dependent manner. The up-regulation of BCL2-associated X (BAX), cyclin dependent kinase inhibitor 1A (P21), Caspase 3 (CASP3), and Caspase 9 (CASP9), the down-regulation of BCL2 apoptosis regulator (BCL2), Annexin V-FITC/propidium iodide, and reactive oxygen species (ROS) generation results suggested that BC-CuONPs had a significant apoptotic impact when compared to the control. Scratch tests and vascular endothelial growth factor receptor gene (VEGF) down-regulation demonstrated that BC-CuONPs had anti-metastatic activity. The cell cycle analysis and down-regulation of Cyclin D1 (CCND1) and cyclin dependent kinase 4 (CDK4) revealed that cancer cells were arrested in the sub-G1 phase. Finally, the results showed that the secondary metabolites in the supernatant of Bacillus coagulans could form CuONPs, and biogenic BC-CuONPs showed anti-metastasis and anticancer properties on breast cancer cells while having less adverse effects on normal cells. Therefore, the synthesized CuONPs using B. coagulans supernatant can be shown as a potential candidate for a new therapeutic strategy in cancer management.

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“…Bacillus coagulans bacterial supernatant (GBI-30, 6068) was prepared according to the protocol reported in the previous study (27). SKBR3 breast cancer cells (IBRC C10147) and HFF normal cells were purchased from Iran's National Center for Genetic and Biological Resources.…”

Section: Cell Culturementioning

confidence: 99%

“…First, SKBR3 cells were prepared in a 6-well plate as in the previous step and treated with IC50 of bacterial culture supernatant for 48 h. Untreated cells were considered negative controls. RNA extraction, cDNA synthesis, and realtime PCR were performed according to the protocol reported in the previous study (27).…”

Section: Real-time Pcr To Evaluate Gene Expressionmentioning

confidence: 99%

Antiproliferative Potential of Bacillus coagulans Supernatant on SKBR3 Breast Cancer Cell Line

Dolati¹,

Tafvizi²,

Salehipour³

et al. 2022

Iran J Med Microbiol

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Background and Aim: Breast cancer is one of the most common types of cancer among Iranian women. To date, the usual cancer treatments have not been entirely effective. Therefore, creating anticancer products is of great importance. The aim of this study was to evaluate the cytotoxic, anticancer, and induction effects of Bacillus coagulans probiotic bacterial supernatant on SKBR3 cells. Materials and Methods:The anticancer potential and cytotoxic effect of different concentrations of probiotic bacterial supernatants (1, 2, 3, 4, 5, 6 and 7 mg/mL) were evaluated on SKBR3 cells for 24, 48, and 72 h by MTT technique. QRT-RCR was used to assess the expression of bax, bcl2, casp3, and casp9 genes, and flow cytometry was used to evaluate apoptosis in cancer cells. Results:The inhibitory effect of dose-and time-dependent B. coagulans supernatant showed that the supernatant of this probiotic bacterium had a cytotoxic effect on SKBR3 cancer cells. On the other hand, analysis of flow cytometry results and increased expression of bax, casp3, and casp9 pro-apoptotic genes and decreased bcl2 expression in cancer cells showed induction of apoptosis. Conclusion:The anticancer and cytotoxic effect of B. coagulans probiotic bacterial supernatant on SKBR3 cancer cells shows that with further research, this probiotic bacterium can be used as a new strategy for the possible treatment of breast cancer.

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Inhibitory effects of probiotic Bacillus coagulans against MCF7 breast cancer cells

Cited by 17 publications

References 34 publications

Anticancer and antibacterial activities of secondary bioactive compounds from endosymbiotic bacteria of aphid and their surrounding organisms (Predator and protector)

Anticancer and antibacterial activities of secondary bioactive compounds from endosymbiotic bacteria of aphid and their surrounding organisms (Predator and protector)

Biogenic copper oxide nanoparticles from Bacillus coagulans induced reactive oxygen species generation and apoptotic and anti-metastatic activities in breast cancer cells

Antiproliferative Potential of Bacillus coagulans Supernatant on SKBR3 Breast Cancer Cell Line

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