Inhibitory effects of Nelumbo nucifera leaves on rat lens aldose reductase, advanced glycation endproducts formation, and oxidative stress

Jung, Hyun Ah; Jung, Yu Jung; Yoon, Na Young; Jeong, Da Mi; Bae, Hyun Ju; Kim, Dong-Wook; Na, Dong Hee; Choi, Jae Sue

doi:10.1016/j.fct.2008.10.004

Cited by 117 publications

(82 citation statements)

References 45 publications

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“…Also, the HPLC profiles of the active ethyl acetate fraction from Nelumbo nucifera leaves indicated the presence of In terms of N. nucifera's antioxidant effect, the leaf extract exhibited potent antioxidant capacities in the DPPH and total ROS assay. The leaf extracts also showed remarkable inhibitory activities on RLAR and AGE formation [35].…”

Section: Flavones Flavonols Flavanones and Flavanonolesmentioning

confidence: 92%

Plant-Derived Agents with Anti-Glycation Activity

Odjakova¹,

Popova²,

Sharif³

et al. 2012

Glycosylation

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Section: Flavones Flavonols Flavanones and Flavanonolesmentioning

confidence: 92%

Plant-Derived Agents with Anti-Glycation Activity

Odjakova¹,

Popova²,

Sharif³

et al. 2012

Glycosylation

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“…Key words: High performance thin layer chromatography, lotus leaf, nuciferine, quantification, alkaloids *Address for correspondence E-mail: drarunajadhav@gmail.com Nelumbo nucifera is perennial, rhizomatous, aquatic plant belonging to the family of Nelumbonaceae, which has numerous common names (Indian lotus, Chinese water lily and sacred lotus) [1,2] . It is widely grown and consumed over the world, mainly in China, …”

Section: Nafsin and Aruna: Detection And Quantification Of Nuciferinementioning

confidence: 99%

“…Statistical analysis of the data showed that the method is reproducible and selective for the estimation of nuciferine. The developed high-performance thin-layer chromatography method can be used for quantification of nuciferine in different species of lotus and formulations containing nuciferine.Key words: High performance thin layer chromatography, lotus leaf, nuciferine, quantification, alkaloids *Address for correspondence E-mail: drarunajadhav@gmail.com Nelumbo nucifera is perennial, rhizomatous, aquatic plant belonging to the family of Nelumbonaceae, which has numerous common names (Indian lotus, Chinese water lily and sacred lotus) [1,2] . It is widely grown and consumed over the world, mainly in China, …”

mentioning

confidence: 99%

Development and Validation of HPTLC Method for Quantitative Estimation of Nuciferine from Lotus leaves

Patel¹,

Jadhav²

2017

pharmaceutical-sciences

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Nafsin and Aruna: Detection and Quantification of Nuciferine by HPTLCNelumbo nucifera (Nelumbonaceae), commonly known as lotus, is an aquatic perennial plant. A simple, precise and accurate high-performance thin-layer chromatography method for quantitative estimation of an alkaloid, nuciferine from methanol extract of lotus leaf was developed. The method employed high-performance thinlayer chromatography aluminium plate precoated with silica gel 60F 254 (10×10 cm) as stationary phase and separation was achieved by using a suitable mobile phase chloroform:ethylacetate:methanol:glacial acetic acid [6.5:1:2.5:0.025 v/v/v/v] and Dragendorff's reagent was used for detection. Quantitation of the method was achieved by densitometric method at 272 nm using Camag TLC scanner 3. This method showed good separation of nuciferine in the extract with R f value 0.44±0.02. The regression analysis data indicated good linear relationship for the calibration plots for nuciferine in the range of 100-450 ng/spot and regression coefficient was 0.998. The accuracy and reliability of the proposed method was ascertained by evaluating various validation parameters like linearity, precision, accuracy, robustness, specificity, limit of quantification and limit of detection according to the ICH guideline. Statistical analysis of the data showed that the method is reproducible and selective for the estimation of nuciferine. The developed high-performance thin-layer chromatography method can be used for quantification of nuciferine in different species of lotus and formulations containing nuciferine.Key words: High performance thin layer chromatography, lotus leaf, nuciferine, quantification, alkaloids *Address for correspondence E-mail: drarunajadhav@gmail.com Nelumbo nucifera is perennial, rhizomatous, aquatic plant belonging to the family of Nelumbonaceae, which has numerous common names (Indian lotus, Chinese water lily and sacred lotus) [1,2] . It is widely grown and consumed over the world, mainly in China,

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“…These reactive, fluorescent substances are known as AGEs. These are responsible for subsequent damage of the tissues and severe complications of DM 5 . Therefore, there is growing interest in drugs that inhibit AR and AGEs.…”

Section: Introductionmentioning

confidence: 99%

Aldose Reductase Inhibitory Activity of Butea monosperma for the Management of Diabetic Complications

Veeresham¹,

Garapelli²,

Rao³

2015

Pharmacologia

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Background: Aldose Reductase (AR) enzyme and advanced glycation end products (AGEs) play an important role in diabetic complications. The AR and AGEs inhibitors are beneficial in prevention of diabetic complications. Objective: Aim of present study was to evaluate the in vitro and in vivo AR and AGEs inhibitory activity of an antidiabetic plant Butea monosperma. Methods: The methanolic extract, standardized extract of flower and the major constituent butein were studied for their inhibitory activity against Rat Lens AR (RLAR), rat kidney AR and generation of AGEs. In addition, in vivo inhibition of lens galactitol accumulation in galactose-fed rat model was studied. Results:The plant extracts and butein were shown to possess AR inhibitory activity in both in vitro and in vivo assays with equal potency to that of standard quercetin. In case of inhibited AGEs formation, the plant extracts showed insignificant activity where as butein was found to be potent. Conclusion: The results obtained in this study provide a new dimension to the hitherto unknown activity of the plant as possible protective agent against long-term diabetic complications.

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Inhibitory effects of Nelumbo nucifera leaves on rat lens aldose reductase, advanced glycation endproducts formation, and oxidative stress

Cited by 117 publications

References 45 publications

Plant-Derived Agents with Anti-Glycation Activity

Plant-Derived Agents with Anti-Glycation Activity

Development and Validation of HPTLC Method for Quantitative Estimation of Nuciferine from Lotus leaves

Aldose Reductase Inhibitory Activity of Butea monosperma for the Management of Diabetic Complications

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