Inhibitory Effects of Methanol Extract of Kaempferia galanga on melanogenesis in B16/F10 Melanoma Cells

Abstract

Objective: Recently the demands for the effective and safe depigmentative and anti-aging agents of the skin have increased due to the medical, pharmaceutical and cosmetic reasons. The purpose of this study is to investigate the MKG(Methanol Extract of Kaempferia galanga) and their dermal bioactivity properties related to cosmeceuticals such as depigmentation.Methods: We assessed inhibitory effects of MKG on melanin production in B16/F10 melanoma cells, on mushroom tyrosinase activity, effects of MKG on the expression tyrosinase, TRP-1, TRP-2, GSK-3β, CREB, MITF in B16/F10 melanoma cells without cytotoxicity range. Cell viability was measured by MTT assay and tyrosinase activity was assessed using by DOPA staining, western-blot analysis. We measured inhibition of melanin synthesis and tyrosinase activity by down-regulation of melanogenic enzyme expressions in α-MSH induced melanogenesis B16/F10 melanoma cells.Results: MKG inhibited tyrosinase-activity, total melanin contents and dendrite out-growth. MKG inhibited melanogenesis by down-regulation of tyorsinase, TRP-1, TRP-2, CREB, and MITF in B16/F10 cells. The treatment with MKG at the 12.5, 25 ㎍/㎖ level significantly inhibited the melanin synthesis induced α-MSH in B16/F10 melanoma cells compared with untreated control.

Leathesia difformis Extract Inhibits α-MSH-Induced Melanogenesis in B16F10 Cells via Down-Regulation of CREB Signaling Pathway

“…In addition, melanin biosynthesis is crucial to enhance the body’s defense against harmful effects of ultraviolet (UV) radiation and DNA damage to the skin [3,4]. However, excessive melanin production can lead to hyperpigmentation in the form of spots, freckles, and inflammation due to prolonged exposure to UV light [5,6].…”

Mechanical Characteristics and Antibiosis of Sized Fabrics with Bletilla striata

“…Recently, many studies have examined the effects of Bletilla stratia in the medical and skin care fields. Oh (2008) studied the anti-inflammatory activity from an extract of Bletilla striata, and Yoon et al (2003) examined the inhibitive action of melanin pigment with B16 melanomatous cells. In addition, many patents on the whitening or cosmetic development of atopic skin have been granted.…”