Leathesia difformis Extract Inhibits α-MSH-Induced Melanogenesis in B16F10 Cells via Down-Regulation of CREB Signaling Pathway

Seo, Gayoung; Ha, Yuna; Park, Ah-Hyun; Kwon, Oh Wook; Kim, Youn-Jung

doi:10.3390/ijms20030536

Cited by 33 publications

(33 citation statements)

References 54 publications

(62 reference statements)

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“…That is, phosphorylated CREB upregulates the transcription of MITF to ultimately increase the MITF protein expression level. To date, a material with an anti-melanin effect that targets these processes has been identified [ 45 ]. Therefore, LHMW may reduce MITF expression by suppressing adenylate cyclase, PKA, or phosphorylated CREB.…”

Section: Discussionmentioning

confidence: 99%

Lactobacillus helveticus-Fermented Milk Whey Suppresses Melanin Production by Inhibiting Tyrosinase through Decreasing MITF Expression

et al. 2020

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Whey obtained from milk fermented by the Lactobacillus helveticus CM4 strain (LHMW) has been shown to improve skin barrier function and increase skin-moisturizing factors. In this study, we investigated the effects of LHMW on melanin production to explore the additional impacts of LHMW on the skin. We treated mouse B16 melanoma cells with α-melanocyte-stimulating hormone (α-MSH) alone or simultaneously with LHMW and measured the amount of melanin. The amount of melanin in B16 cells treated with α-MSH significantly increased by 2-fold compared with that in control cells, and tyrosinase activity was also elevated. Moreover, treatment with LHMW significantly suppressed the increase in melanin content and elevation of tyrosinase activity due to α-MSH. LHMW also suppressed the α-MSH-induced increased expression of tyrosinase, tyrosinase-related protein 1 (TRP1), and dopachrome tautomerase (DCT) at the protein and mRNA levels. Furthermore, the mRNA and protein microphthalmia-associated transcription factor (MITF) expression levels were significantly increased with treatment with α-MSH alone, which were also suppressed by LHMW addition. LHMW suppression of melanin production is suggested to involve inhibition of the expression of the tyrosinase gene family by lowering the MITF expression level. LHMW may have promise as a material for cosmetics with expected clinical application in humans.

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Section: Discussionmentioning

confidence: 99%

Lactobacillus helveticus-Fermented Milk Whey Suppresses Melanin Production by Inhibiting Tyrosinase through Decreasing MITF Expression

et al. 2020

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“…Besides irradiation, another widely used inducer of melanogenesis is 3-isobutyl-1-methylxanthine (IBMX), which performs this function as an inhibitor of the phosphodiesterase enzyme, increasing intracellular cAMP and thus inducing MITF. IBMX has been described as an inducer of melanogenesis in reconstructed human skin models [ 20 ], in immortalized murine melanocytes (Melan-A cell) [ 21 ], and in B16-F10 melanomas [ 22 ]. IBMX is considered the main positive control for the discovery of compounds with pigmentation inhibiting activity.…”

Section: Discussionmentioning

confidence: 99%

A 2D and 3D melanogenesis model with human primary cells induced by tyrosine

Branquinho

Silva

et al. 2020

J Biol Methods

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Research on melanogenesis, its regulation in health and disease, and the discovery of new molecules with pigmenting and depigmenting activities use different models. Here we standardize a protocol based on previous ones using primary human melanocytes and keratinocytes in co-cultures, in which melanogenesis was induced under mild conditions by the addition of tyrosine plus ammonium chloride (NH4Cl). The expression of MITF, TYR, TYRP1, and Melan-A as well as melanin content were measured. Furthermore, we extended this study to a reconstructed 3D model. Pigmentation was visually observable and melanosomes were identified by Fontana-Masson staining by the addition of tyrosine plus NH4Cl during the stratification phase. The 2D and 3D protocols proposed here circumvent limitations of previous models, using human primary cells and mild conditions for melanogenesis. These protocols offer a viable, robust, simple, and animal-free investigational option for human skin pigmentation studies and screening tests for new compounds that modulate pigmentation.

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“…MITF regulates the expression of tyrosinase, tyrosinase-related protein 1,2 which is required for melanogenesis. Ethyl acetate fraction of Leathesia difformis Areschoug showed the effect on melanin synthesis and cellular tyrosinase activity by downregulating the CREB, PKA, and cAMP pathways [75]. ERK pathway involves in anti-melanogenesis.…”

Section: Tyrosinase Inhibition Activity Of Seaweedmentioning

confidence: 99%

Potential Use of Seaweed Bioactive Compounds in Skincare—A Review

et al. 2019

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Modern lifestyles have developed new attention on appearance and personal care which attract a huge number of consumers towards cosmetic products. The demand for a skincare product with natural ingredients is rapidly increasing. Seaweeds are major resources for in-demand active compounds with a wide variety of applications. The use of seaweed-derived ingredients in cosmetic products has increased in recent years as many scientific studies have proved the potential skincare properties of seaweed bioactive compounds. This review emphasizes possible skincare properties of seaweed bioactive compounds. The review outlines the mechanism involved in skin problems including hyperpigmentation, premature skin aging, and acne in the first part while the second part focuses on the promising application of seaweeds in skin protection by highlighting the bioactive compound responsible for their bioactivity.

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Leathesia difformis Extract Inhibits α-MSH-Induced Melanogenesis in B16F10 Cells via Down-Regulation of CREB Signaling Pathway

Cited by 33 publications

References 54 publications

Lactobacillus helveticus-Fermented Milk Whey Suppresses Melanin Production by Inhibiting Tyrosinase through Decreasing MITF Expression

Lactobacillus helveticus-Fermented Milk Whey Suppresses Melanin Production by Inhibiting Tyrosinase through Decreasing MITF Expression

A 2D and 3D melanogenesis model with human primary cells induced by tyrosine

Potential Use of Seaweed Bioactive Compounds in Skincare—A Review

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