Inhibitory effects of LiCl on replication of type II porcine reproductive and respiratory syndrome virus in vitro

Cui, Jin; Xie, Jiexiong; Gao, Ming; Zhou, Han; Chen, Yao; Cui, Tiantian; Bai, Xiaolei; Wang, Heng; Zhang, Guihong

doi:10.3851/imp2924

Cited by 24 publications

(20 citation statements)

References 33 publications

(39 reference statements)

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“…More recently, several reports have demonstrated the antiviral effect of LiCl on DNA viruses, such as herpes simplex virus [33], pseudorabies herpesvirus [30], and porcine parvovirus [7]. Lately, the antiviral effect of LiCl on RNA viruses, such as bronchitis coronavirus [13,16], transmissible gastroenteritis virus [27], and type II porcine reproductive and respiratory syndrome virus [18], has also been demonstrated. These reports indicate that LiCl might be a potential antiviral drug for other viruses.…”

Section: Introductionmentioning

confidence: 99%

Antiviral effect of lithium chloride on infection of cells by canine parvovirus

Zhou

Yan

et al. 2015

Arch Virol

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Canine parvovirus type 2 causes significant viral disease in dogs, with high morbidity, high infectivity, and high mortality. Lithium chloride is a potential antiviral drug for viruses. We determined the antiviral effect of Lithium Chloride on canine parvovirus type 2 in feline kidney cells. The viral DNA and proteins of canine parvovirus were suppressed in a dose-dependent manner by lithium chloride. Further investigation verified that viral entry into cells was inhibited in a dose-dependent manner by lithium chloride. These results indicated that lithium chloride could be a potential antiviral drug for curing dogs with canine parvovirus infection. The specific steps of canine parvovirus entry into cells that are affected by lithium chloride and its antiviral effect in vivo should be explored in future studies.

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Section: Introductionmentioning

confidence: 99%

Antiviral effect of lithium chloride on infection of cells by canine parvovirus

Zhou

Yan

et al. 2015

Arch Virol

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“…[5][6][7][8] Recently, some reports showed that LiCl had antiviral effects against a number of viruses, such as bronchitis coronavirus (IBV), an avian coronavirus, transmissible gastroenteritis virus (TGEV), pseudorabies herpesvirus in vitro [9][10][11] and, EV-A71, a virus which also belong to the family Picornaviridae. 12 And some new reports: porcine reproductive and respiratory syndrome virus (PRRSV), 13,14 and mammalian orthoreoviruses, 15 this suggests that LiCl has the potential to become an antiviral drug. Nevertheless, it is unclear that whether LiCl had an inhibitory effect on the FMDV.…”

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confidence: 99%

Lithium chloride inhibits early stages of foot-and-mouth disease virus (FMDV) replication in vitro

et al. 2017

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Foot-and-mouth disease virus (FMDV) causes an economically important and highly contagious disease of cloven-hoofed animals such as cattle, swine, and sheep. FMD vaccine is the traditional way to protect against the disease, which can greatly reduce its occurrence. However, the use of FMD vaccines to protect early infection is limited. Therefore, the alternative strategy of applying antiviral agents is required to control the spread of FMDV in outbreak situations. As previously reported, LiCl has obviously inhibition effects on a variety of viruses such as transmissible gastroenteritis virus (TGEV), infectious bronchitis coronavirus (IBV), and pseudorabies herpesvirus and EV-A71 virus. In this study, our findings were the first to demonstrate that LiCl inhibition of the FMDV replication. In this study, BHK-21 cell was dose-dependent with LiCl at various stages of FMDV. Virus titration assay was calculated by the 50% tissue culture infected dose (TCID ) with the Reed and Muench method. The cytotoxicity assay of LiCl was performed by the CCK8 kit. The expression level of viral mRNA was measured by RT-qPCR. The results revealed LiCl can inhibit FMDV replication, but it cannot affect FMDV attachment stage and entry stage in the course of FMDV life cycle. Further studies confirmed that the LiCl affect the replication stage of FMDV, especially the early stages of FMDV replication. So LiCl has potential as an effective anti-FMDV drug. Therefore, LiCl may be an effective drug for the control of FMDV. Based on that, the mechanism of the antiviral effect of LiCl on FMDV infection is need to in-depth research in vivo.

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“…The relative viral mRNA level decreased by more than 30% (p < 0.001) at the concentration of 10 mM and by 50% at 20 mM (p < 0.001). The authors, however, cautioned that the effect might have been dependent on LiCl presence during the early stages of viral replication (first 9 hours) and the increase of tumor necrosis factor-α, which was greater following LiCl alone than induced by the virus 2 . In vitro studies of another porcine coronavirus causing transmissible gastroenteritis indicated that LiCl (5-25 mM) acts on both early and late stages of infection and inhibits apoptosis 3 .…”

Section: Resultsmentioning

confidence: 99%

Lithium and coronaviral infections. A scoping review.

Nowak

Walkowiak²

2020

F1000Res

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The current rapid spread of the novel coronavirus (SARS-CoV-2) causing coronavirus disease 2019 (COVID-19) calls for a rapid response from the research community. Lithium is widely used to treat bipolar disorder, but has been shown to exhibit antiviral activity. This brief review took a systematic approach to identify six in vitro studies reporting on the influence of lithium on coronaviral infections. We propose mechanistic investigation of the influence of lithium – alone and with chloroquine – on the SARS-CoV-2 infection.

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Inhibitory effects of LiCl on replication of type II porcine reproductive and respiratory syndrome virus in vitro

Abstract: Our findings imply that the LiCl has the potential to be used as anti-PRRSV therapy.

Cited by 24 publications

References 33 publications

Antiviral effect of lithium chloride on infection of cells by canine parvovirus

Antiviral effect of lithium chloride on infection of cells by canine parvovirus

Lithium chloride inhibits early stages of foot-and-mouth disease virus (FMDV) replication in vitro

Lithium and coronaviral infections. A scoping review.

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