Antiviral effect of lithium chloride on infection of cells by canine parvovirus

Zhou, Pei; Fu, Xinliang; Yan, Zhongshan; Fang, Bo; San, Huang; Fu, Cheng; Hong, Malin

doi:10.1007/s00705-015-2577-x

Cited by 12 publications

(13 citation statements)

References 31 publications

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“…There are several studies exploring the roles of LiCl on virus infection and suggesting that LiCl inhibit certain virus replication, such as transmissible gastroenteritis coronavirus, HIV, canine parvovirus, PRRSV and so on [8][9][10][11][12][13][14][15]. In this study, we demonstrate that LiCl can also restrict CVB3 infection in vivo and in vitro.…”

Section: Discussionsupporting

confidence: 53%

“…As previously reported, 10-60 mM was applied as nontoxic concentration range of LiCl for antiviral effect in F81 cells [14]. So we firstly detected the cytotoxicity of LiCl in CVB3-replicated HeLa or 293T cells.…”

Section: Cytotoxicity Of Licl In Hela and 293t Cellsmentioning

confidence: 90%

“…So LiCl has been explored for treating diabetes, Alzheimer's disease and ovarian cancer, as well as antimicrobial infection [7]. Prior investigations have also revealed that LiCl exhibits antiviral effects on certain viruses, e.g., coronavirus, herpes simplex, infectious bronchitis virus, porcine reproductive and respiratory syndrome (PRRS) and feline calicivirus [8][9][10][11][12][13][14][15].…”

Section: Introductionmentioning

confidence: 99%

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Lithium chloride confers protection against viral myocarditis via suppression of coxsackievirus B3 virus replication

Zhao

Yan

Wang

et al. 2020

Microbial Pathogenesis

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Lithium chloride confers protection against viral myocarditis via suppression of coxsackievirus B3 virus replication, Microbial Pathogenesis, https://doi. Highlights Lithium chloride inhibits coxsackievirus B3 virus replication in vitro and in vivo.Lithium chloride inhibits CVB3 replication via inhibiting virus-induced cell apoptosis.LiCl treatment in vivo obviously alleviated CVB3-induced acute myocarditis. 3 Abstract Viral myocarditis (VMC) is a type of inflammation affecting myocardial cells caused by viral infection and has been an important cause of dilated cardiomyopathy (DCM) worldwide. Type B3 coxsackievirus (CVB3), a non-enveloped positive-strand RNA virus of the Enterovirus genus, is one of most common agent of viral myocarditis. Till now, effective treatments for VMC are lacking due to lack of drugs or vaccine. Lithium chloride (LiCl) is applied in the clinical management of manic depressive disorders. Accumulating evidence have demonstrated that LiCl, also as an effective antiviral drug, exhibited antiviral effects for specific viruses. However, there are few reports of evaluating LiCl's antiviral effect in mice model. Here, we investigated the inhibitory influence of LiCl on the CVB3 replication in vitro and in vivo and the development of CVB3-induced VMC. We found that LiCl significantly suppressed CVB3 replication in HeLa via inhibiting virus-induced cell apoptosis. Moreover, LiCl treatment in vivo obviously inhibited virus replication within the myocardium and alleviated CVB3-induced acute myocarditis. Collectively, our data demonstrated that LiCl inhibited CVB3 replication and negatively regulated virus-triggered inflammatory responses.Our finding further expands the antiviral targets of LiCl and provides an alternative agent for viral myocarditis. Conflict of interestThe authors declare no conflict of interest. Ethical approvalThe animal experiments were performed in accordance with Soochow University institutional guidelines, and the study was approved by the Ethics Committee of Soochow University in written form. Euthanasia of mice was performed by carbon dioxide inhalation with minimum fear, anxiety and pain. HighlightsLithium chloride inhibits coxsackievirus B3 virus replication in vitro and in vivo.

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Section: Discussionsupporting

confidence: 53%

Section: Cytotoxicity Of Licl In Hela and 293t Cellsmentioning

confidence: 90%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Lithium chloride confers protection against viral myocarditis via suppression of coxsackievirus B3 virus replication

Zhao

Yan

Wang

et al. 2020

Microbial Pathogenesis

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“…Antiviral effects of LiCl have been extensively investigated. LiCl can inhibit the infection of some DNA and RNA viruses, such as herpes simplex virus (Skinner et al, 1980), canine parvovirus (Zhou et al, 2015), porcine parvovirus (Chen et al, 2015), pseudorabies herpesvirus (Sui et al, 2010), porcine reproductive and respiratory syndrome virus (Hao et al, 2015), feline calicivirus (Wu et al, 2015), infectious bronchitis virus, an avian coronavirus (Li et al, 2009) and transmissible gastroenteritis virus (TGEV), a porcine coronavirus (Ren et al, 2011). Both PEDV and TGEV are classified into group I of the genus Alphacoroavirus.…”

Section: Introductionmentioning

confidence: 99%

Antiviral effect of lithium chloride on porcine epidemic diarrhea virus in vitro

Gao

et al. 2018

Research in Veterinary Science

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Porcine epidemic diarrhea virus (PEDV), a member of the Coronaviridae family, causes acute diarrhea, vomiting, dehydration, and high mortality rates in neonatal piglets. Severe outbreaks of PEDV variants have re-emerged in Asia and North America since 2010, causing tremendous economic losses to the swine industry. The lack of effective therapeutic treatment promotes the research for new antivirals. Lithium chloride (LiCl) has been reported as a potential antiviral drug for certain viruses. In this study, the antiviral effect of LiCl on PEDV in Vero cells was evaluated. Real-time quantitative PCR and indirect immunofluorescence assay indicated that LiCl effectively inhibited the entry and replication of PEDV in Vero cells. The expression of viral RNA and protein of PEDV in Vero cells was suppressed in a dose-dependent manner by LiCl. Moreover, addition of LiCl inhibited both early and late cell apoptosis induced by PEDV. Our data implied that LiCl could be a potential antiviral drug against PEDV infection. Further studies are required to explore the antiviral effect of lithium chloride on PEDV infection in vivo.

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“…VP2 protein had been investigated as a candidate potential vaccine antigen due to its good immunogenicity [11]. CPV-2, first identified and described in 1978 in both the United States and Australia, is closely related to feline panleukopenia virus (FPV) [12]. A few years after its emergence, the original virus type CPV-2 was replaced worldwide by three new antigenic variants (i.e., CPV-2a, CPV-2b and CPV-2c) based on aa substitutions (CPV-2a: Val-555-Ile, Asp-305-Tyr, Ala-300-Gly, Ile-101-Thr and Met-87-Leu; CPV-2b: Ile-555-Val and Asp-426-Asn; CPV-2c: Asp-426-Glu) in the VP2 gene [12][13][14][15][16][17][18].…”

Section: Introductionmentioning

confidence: 99%

Occurrence of canine parvovirus in dogs from Henan province of China in 2009–2014

et al. 2016

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BackgroundThere is no information concerning the genotype of Canine parvovirus (CPV) currently circulating in Henan province, China. Therefore, the aim of the present study was to provide insights into the epidemiology and molecular characterization of CPV circulating in Henan province from 2009 to 2014.ResultsNineteen thousand nine hundred seven dogs from pet hospitals in the cities of Luoyang, Anyang, Jiaozuo, Sanmenxia, Xinxiang, Zhengzhou in Henan province between 2009 and 2014 were investigated. Over the 6-year period, 1169 CPV-positive cases were identified and the morbidity of CPV infection ranged from 4.16 to 8.06 %, although morbidity was not significant (P > 0.05) between 2009 and 2014. Factors associated with morbidity included sampling season, dog age, breed, vaccination status, and sex. CPV co-infection with coccidium (10.00 %), canine distemper virus (4.79 %), hookworm (2.40 %), canine coronavirus (1.11 %), roundworm (1.03 %), tapeworm (0.17 %) and Babesia spp. (0.09 %) were observed. The new CPV-2a variant was more prevalent than the new CPV-2b variant in Henan province. CPV 2c was not observed in this study.ConclusionsThe epidemiology of CPV infection and identification of the circulating genotypes in Henan province, China from 2009 to 2014 determined that the new CPV-2a variant was more prevalent.

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Antiviral effect of lithium chloride on infection of cells by canine parvovirus

Cited by 12 publications

References 31 publications

Lithium chloride confers protection against viral myocarditis via suppression of coxsackievirus B3 virus replication

Lithium chloride confers protection against viral myocarditis via suppression of coxsackievirus B3 virus replication

Antiviral effect of lithium chloride on porcine epidemic diarrhea virus in vitro

Occurrence of canine parvovirus in dogs from Henan province of China in 2009–2014

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