Inhibitory effects ofPinus massonianabark extract on hepatitis C virusin vitro

Abstract

Context: Given that Pinus massoniana Lamb (Pinaceae) bark extract (PMBE) is a safe and non-toxic flavonoid found abundantly in nature, it was considered a promising novel candidate agent in the treatment of virus infection. Object: Experiments were conducted to assay the antiviral character of PMBE against Hepatitis C virus (HCV). Materials and methods: Assay of PMBE cytotoxicity, HCV replication, infectious HCV production, and its potential influence on the pathways for IFN-ISRE and NS3 protease were conducted. Results: HCV replication was suppressed when the concentration of PMBE raised greater than 5 mg/mL and its EC 50 was 9.58 mg/mL. In the 10 mg/mL group, HCV replication was suppressed for 48 h. When the concentration increased to 40 mg/mL, HCV replication was significantly suppressed (luciferase activity was only 10% at 96 h). PMBE could inhibit HCV virus production efficiently (PMBE group was 5 FFU). Cell viability was affected by 40 mg/mL of PMBE. The F/R ratio ranged from 98% to 101%. The rate of OD 450 ranged from 96% to 102%. NS3 catalytic activity ranged from 5% (40 mg/mL PMBE) to 45% (5 mg/mL PMBE). Even when used in a low amount (5 mg/mL), NS3 catalytic activity was significantly inhibited (p50.01). Conclusions:The results suggest that PMBE is effective for use in the stabilization of HCV replication and active liver inflammation.

Pinus massoniana bark extract inhibits migration of the lung cancer A549 cell line

“…PMBE has previously been used in traditional Chinese medicine for patients with cancer (15,25,26). The present study investigated the effect of PMBE on lung cancer cells and the potential mechanisms underlying this process.…”

“…The primary active component of PMBE is anthocyanin series B (15). Certain studies have reported that PMBE induces the apoptosis of hepatoma and cervical cancer cells (16,17).…”

The long and stumble way to find potential active compounds from plants for defeating hepatitis B and C: review

“…Limonium sinense root extract can also inhibit viral entry of HCV to the cell. Extract of Dimocarpus longan can also inhibit HCV (Kim et al 2013;Kong et al 2013;Choi et al 2014;Hsu et al 2015;Wang et al 2015;Apriyanto et al 2016).…”

The Function of some Important TCM and Iranian Medicinal Plants in Treatment of Viral Hepatitis

“…Herbal formula for treating hepatitis A consist of Taraxacum officinale (dandelion) + Andrographis paniculata (kalmegh) + Artemisia ludoviciana (western mugwort) + Ligusticum porteri + Glycyrrhiza glabra. 99 The most important medicinal plants against HCV activities are Trichilia dregeana, Detarium microcarpum, Pragmanthera capitata, 100 Ruta angustifolia, 101 Glycyrrhiza uralensis, 102 Toona sureni, Melicope latifolia, Melanolepis multiglandilosa, and Ficus fistula, 103 Ligustrum lucidum, 104 Platycodon grandiflorum, 105 Embelia ribes, 106 Phyllanthus amarus, 107 Garcinia mangostana, 108 Pinus massoniana, 109 Acacia nilotica, Piper cubeba, and Syzygium aromaticum, 110 Limonium sinense, 111 Morinda citrifolia, 112 and Dimocarpus longan. 113 These medicinal herbs and plants are more affordable and cost effective than conventional medicine.…”