Inhibitory Effects of Hybrid Liposomes Composed of Phosphatidylcholine and Docosahexaenoic Acid on the Growth of Colon Cancer Cells along with Apoptosis and Differentiation

Goto, Kōichi; Zako, Keiko; Komizu, Yuji; Ueoka, Ryuichi

doi:10.1246/cl.2011.90

Cited by 3 publications

(7 citation statements)

References 29 publications

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“…It has been already reported that the diameters of DMPC/DHA composed of 10-50 mol% DHA were 60-100 nm and remained stable for 3 weeks. 19) Here, DMPC/DHA composed of 50 mol% DMPC and 50 mol% DHA were prepared by the sonication method Engineering, Sojo University; 4-22-1 Ikeda, Kumamoto 860-0082, Japan. Received December 27, 2010; accepted February 25, 2011; published online March 7, 2011 Therapeutic effects of hybrid liposomes (L-a a-dimyristoylphosphatidylcholine (DMPC)/docosahexaenoic acid (DHA)) composed of 50 mol% DMPC and 50 mol% DHA on the metastasis of human colon carcinoma (HCT116) cells were examined in vivo.…”

Section: Methodsmentioning

confidence: 99%

“…It has been already reported that the diameters of DMPC/DHA composed of 10-50 mol% DHA were 60-100 nm and remained stable for 3 weeks. 19) Here, DMPC/DHA composed of 50 mol% DMPC and 50 mol% DHA were prepared by the sonication method and employed in vivo experiments. The liposomes composed of DMPC (DMPC liposomes) were prepared in the same manner as described above.…”

Section: Methodsmentioning

confidence: 99%

Section: )mentioning

confidence: 99%

“…In the course of our study for HL, the following interest- 23) In addition, we elucidated the remarkably inhibitory effects on the growth of human colon cancer (HCT116) cells along with apoptosis and differentiation by HL including DHA in vitro. 19) However, therapeutic effects of HL including DHA in vivo have not yet been examined.In this study, we investigated the therapeutic effects of DMPC/DHA hybrid liposomes composed of 50 mol% L-adimyristoylphosphatidylcholine (DMPC) and 50 mol% DHA on the hepatic metastasis mouse models of human colon carcinoma HCT116 cells in vivo. Furthermore, the therapeutic effects on the hepatic metastasis mouse models by intravenous treatment of DMPC/DHA were revealed on the basis of histological analysis of liver tissues.…”

mentioning

confidence: 99%

“…(a) Stereochemical control of the enantioselective hydrolysis of amino acid esters could be established by temperature regulation and changing the composition of the HL. 13,14) (b) Inhibitory effects of HL including antitumor drugs, 15) sugar surfactants 16) or polyunsaturated fatty acids [17][18][19] have been observed on the growth of tumor cells in vitro and in vivo. (c) High inhibitory effects of HL on the growth of human leukemia, 20) lung carcinoma 21) and breast tumor 22) cells in vitro along with the induction of apoptosis have been obtained without using drugs.…”

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Therapeutic Effects of Hybrid Liposomes Composed of Phosphatidylcholine and Docosahexaenoic Acid on the Hepatic Metastasis of Colon Carcinoma along with Apoptosis in Vivo

Ichihara

Zako

Komizu

et al. 2011

Biological & Pharmaceutical Bulletin

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Cancer metastasis accounts for the majority of cancer-related deaths. One of the most important strategies for cancer therapy is the control of cancer metastasis. The liver is the most common organ for the metastasis of cancers in the digestive system, and the prognosis for cases with liver metastasis is extremely poor.1-4) Chemotherapy with fluorouracil, irinotecan, and leucovorin has been introduced to improve the postoperative prognosis.5) While the chemotherapy drugs kill tumor cells, they also damage normal cells, causing severe side-effects such as gastrointestinal dysfunction and bone marrow toxicity. Therefore, a chemotherapy that is effective for the metastasis of cancers without any side-effects is required. Docosahexaenoic acid (DHA) is an w-3 polyunsaturated long-chain fatty acid (w-3 PUFAs), whose antitumoral actions have been widely demonstrated in epidemiological and experimental studies. 6) For example, the diets containing fish oil rich in DHA had inhibitory effects against colon tumorigenesis.7-10) Furthermore, it was reported that DHA inhibited the growth of various cancer cells by cytotoxicity of the lipid peroxidation 11) and by apoptotic cell death in vitro. 12)This research suggests that DHA could be an effective drug for cancer chemotherapy. In addition, the relationships between dietary DHA and tumorigenesis have been attracting much attention from many researchers in food and medical science. However, there are few studies on the therapeutic effects of DHA on the growth of tumor cells in vivo. In particular, the therapeutic effects on the mouse models of carcinoma by intravenous treatment of DHA in vivo have not yet been examined due to the poor solubility in water.On the other hand, we have produced hybrid liposomes (HL) which can be prepared by just the sonication of vesicular and micellar molecules in a buffer solution. 13,14) HL are free from any contamination with organic solvents and remain stable for longer periods. The physical properties of these liposomes such as size, membrane fluidity, phase transition temperature, and hydrophobicity can be controlled by changing the constituents and compositional ratios of the HL. In the course of our study for HL, the following interest- 23) In addition, we elucidated the remarkably inhibitory effects on the growth of human colon cancer (HCT116) cells along with apoptosis and differentiation by HL including DHA in vitro. 19) However, therapeutic effects of HL including DHA in vivo have not yet been examined.In this study, we investigated the therapeutic effects of DMPC/DHA hybrid liposomes composed of 50 mol% L-adimyristoylphosphatidylcholine (DMPC) and 50 mol% DHA on the hepatic metastasis mouse models of human colon carcinoma HCT116 cells in vivo. Furthermore, the therapeutic effects on the hepatic metastasis mouse models by intravenous treatment of DMPC/DHA were revealed on the basis of histological analysis of liver tissues. MATERIAL AND METHODSPreparation of DMPC/DHA DMPC/DHA were prepared by sonication of a mixture containing DMPC (N...

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Section: Methodsmentioning

confidence: 99%

“…It has been already reported that the diameters of DMPC/DHA composed of 10-50 mol% DHA were 60-100 nm and remained stable for 3 weeks. 19) Here, DMPC/DHA composed of 50 mol% DMPC and 50 mol% DHA were prepared by the sonication method and employed in vivo experiments. The liposomes composed of DMPC (DMPC liposomes) were prepared in the same manner as described above.…”

Section: Methodsmentioning

confidence: 99%

Section: )mentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

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Therapeutic Effects of Hybrid Liposomes Composed of Phosphatidylcholine and Docosahexaenoic Acid on the Hepatic Metastasis of Colon Carcinoma along with Apoptosis in Vivo

Ichihara

Zako

Komizu

et al. 2011

Biological & Pharmaceutical Bulletin

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Integration analysis for novel lncRNA markers predicting tumor recurrence in human colon adenocarcinoma

Chen

Deng

et al. 2019

J Transl Med

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Background Numerous evidence has suggested that long non-coding RNA (lncRNA) acts an important role in tumor biology. This study focuses on the identification of novel prognostic lncRNA biomarkers predicting tumor recurrence in human colon adenocarcinoma. Methods We obtained the research data from The Cancer Genome Atlas (TCGA) database. The interaction among different expressed lncRNA, miRNA and mRNA markers between colon adenocarcinoma patients with and without tumor recurrence were verified with miRcode, starBase and miRTarBase databases. We established the lncRNA–miRNA–mRNA competing endogenous RNA (ceRNA) network based on the verified association between the selected markers. We performed the functional enrichment analysis to obtain better understanding of the selected lncRNAs. Then we use multivariate logistic regression to identify the prognostic lncRNA markers with covariates. We also generated a nomogram predicting tumor recurrence risk based on the identified lncRNA biomarkers and clinical covariates. Results We included 12,727 lncRNA, 1881 miRNA and 47,761 mRNA profiling and clinical features for 113 colon adenocarcinoma patients obtained from the TCGA database. After filtration, we used 37 specific lncRNAs, 60 miRNAs and 148 mRNAs in the ceRNA network analysis. We identified five lncRNAs as prognostic lncRNA markers predicting tumor recurrence in colon adenocarcinoma, in which four of them were identified for the first time. Finally, we generated a nomogram illustrating the association between the identified lncRNAs and the tumor recurrence risk in colon adenocarcinoma. Conclusions The four newly identified lncRNA biomarkers might be potential prognostic biomarkers predicting tumor recurrence in colon adenocarcinoma. We recommend that further clinical and fundamental researches be conducted on the identified lncRNA markers.

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Lipid Nanoparticle Technology for Delivering Biologically Active Fatty Acids and Monoglycerides

Tan

Yoon

Cho

et al. 2021

IJMS

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There is enormous interest in utilizing biologically active fatty acids and monoglycerides to treat phospholipid membrane-related medical diseases, especially with the global health importance of membrane-enveloped viruses and bacteria. However, it is difficult to practically deliver lipophilic fatty acids and monoglycerides for therapeutic applications, which has led to the emergence of lipid nanoparticle platforms that support molecular encapsulation and functional presentation. Herein, we introduce various classes of lipid nanoparticle technology and critically examine the latest progress in utilizing lipid nanoparticles to deliver fatty acids and monoglycerides in order to treat medical diseases related to infectious pathogens, cancer, and inflammation. Particular emphasis is placed on understanding how nanoparticle structure is related to biological function in terms of mechanism, potency, selectivity, and targeting. We also discuss translational opportunities and regulatory needs for utilizing lipid nanoparticles to deliver fatty acids and monoglycerides, including unmet clinical opportunities.

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Inhibitory Effects of Hybrid Liposomes Composed of Phosphatidylcholine and Docosahexaenoic Acid on the Growth of Colon Cancer Cells along with Apoptosis and Differentiation

Cited by 3 publications

References 29 publications

Therapeutic Effects of Hybrid Liposomes Composed of Phosphatidylcholine and Docosahexaenoic Acid on the Hepatic Metastasis of Colon Carcinoma along with Apoptosis in Vivo

Therapeutic Effects of Hybrid Liposomes Composed of Phosphatidylcholine and Docosahexaenoic Acid on the Hepatic Metastasis of Colon Carcinoma along with Apoptosis in Vivo

Integration analysis for novel lncRNA markers predicting tumor recurrence in human colon adenocarcinoma

Lipid Nanoparticle Technology for Delivering Biologically Active Fatty Acids and Monoglycerides

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