Inhibitory Effects of Helianthus tuberosus Ethanol Extract on Dermatophagoides farina body-induced Atopic Dermatitis Mouse Model and Human Keratinocytes

Abstract

Atopic dermatitis (AD) is a chronic inflammatory skin disease characterized by complex symptoms. To treat AD without adverse effects, alternative therapeutic agents are required. The tubers of Helianthus tuberosus L. (Jerusalem artichoke) have been used in folk remedies for diabetes and rheumatism. However, its effect on AD development remains unknown. Therefore, this study examined the inhibitory effect of H. tuberosus (HT) on AD skin symptoms using an NC/Nga mouse model and HaCaT keratinocytes. The effect of HT and associated molecular mechanisms were evaluated in Dermatophagoides farina body (Dfb)-induced AD mice and tumor necrosis factor (TNF)-α/interferon (IFN)-γ-stimulated HaCaT keratinocytes by ELISA, western blot, and histological analysis. Topical HT administration attenuated AD skin symptoms in Dfb-induced AD mice, with a significant reduction in the dermatitis score and production of inflammatory mediators. HT also decreased epidermal thickness and mast cell infiltration. Moreover, HT restored filaggrin expression and inhibited adhesion molecules in the mice. These effects were confirmed in vitro. Furthermore, HT suppressed the activation of NF-κB, Akt, and mitogen-activated protein kinase (MAPK) signaling pathways induced by TNF-α/IFN-γ. These results suggest that HT is a potential therapeutic agent or supplement for skin allergic inflammatory diseases such as AD.

Defect-Rich La2O3 Nanoparticles with Antioxidant Activity for Human Keratinocytes

“…The interaction of UV radiation with skin chromophores results in excess production of ROS that can interact with DNA, proteins, and other complex organelles inside cells. This can result in DNA mutations, loss of proteins and mitochondria dysfunction affecting keratinocytes and melanocytes and potentially lead to epithelial carcinomas and melanoma, respectively. − …”

Effects of Deacetylasperulosidic Acid on Atopic Dermatitis through Modulating Immune Balance and Skin Barrier Function in HaCaT, HMC-1, and EOL-1 Cells

“…The increase in the level of cytokines released from Th2 cells in AD causes a Th1/Th2 immune imbalance and leads to a lower expression of skin barrier proteins [ 7 ]. It has been reported that damage to the skin barrier function is closely related to AD [ 8 , 9 , 10 , 11 , 12 , 13 ] as it can worsen AD through inflammatory reactions related to AD [ 4 ]. Therefore, the restoration of Th1/Th2 immune balance and skin barrier function is the objective of AD treatment.…”

Therapeutic Effect of Rumex japonicus Houtt. on DNCB-Induced Atopic Dermatitis-Like Skin Lesions in Balb/c Mice and Human Keratinocyte HaCaT Cells

“…In addition, the RJ-treated group reduced ear thickness in a dose-dependent manner during the whole study period. Since AD often develops as a systemic immune response, it can affect the immune organs [14,15,16,17]. Therefore, we evaluated that weight of the lymph nodes and spleen was measured in the final week to examine whether the topical application of RJ provided an anti-AD effect in mice.…”