Inhibitory effects of Gleditsia sinensis fruit extract on telomerase activity and oncogenic expression in human esophageal squamous cell carcinoma

Tang, Wing Ka; Chui, Chung Hin; Fatima, Sarwat; Kok, Stanton Hon Lung; Pak, K.C.; Ou, Tian Miao; Hui, Kin S; Wong, Man Sau; Wong, John; Law, Simon; Tsao, Sai‐Wah; Lam, Alfred King‐Yin; Beh, SL; Srivastava, Gopesh; Ho, Kin‐Yip; Chan, Albert S. C.; Tang, Johnny Cheuk On

doi:10.3892/ijmm.19.6.953

Cited by 18 publications

(17 citation statements)

References 36 publications

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“…We have first explored the oncogenic properties and altered expression of FAM134B (JK1) gene in oesophageal squamous cell carcinoma (Tang et al, 2007). Also, FAM134B has been reported to have roles in pathogenesis as well as prediction of prognosis of other gastrointestinal cancer, namely colorectal cancer (Kasem et al, 2014).…”

Section: Principlementioning

confidence: 99%

Detection of regional DNA methylation using DNA-graphene affinity interactions

Haque

Gopalan

Yadav

et al. 2017

Biosensors and Bioelectronics

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We report a new method for the detection of regional DNA methylation using basedependent affinity interaction (i.e., adsoption) of DNA with graphene. Due to the strongest adsorption affinity of guanine bases towards graphene, bisulfite-treated guanine-enriched methylated DNA leads to a larger amount of the adsorbed DNA on the graphene-modified 2 electrodes in comparison to the adenine-enriched unmethylated DNA. The level of the methylation is quantified by monitoring the differential pulse voltammetric current as a function of the adsorbed DNA. The assay is sensitive to distinguish methylated and unmethylated DNA sequences at single CpG resolution by differentiating changes in DNA methylation as low as 5%. Furthermore, this method has been used to detect methylation levels in a collection of DNA samples taken from oesophageal cancer tissues.

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Section: Principlementioning

confidence: 99%

Detection of regional DNA methylation using DNA-graphene affinity interactions

Haque

Gopalan

Yadav

et al. 2017

Biosensors and Bioelectronics

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“…However, several studies investigated the effect of other herbal extracts on telomerase expression and concentration in PCa cell lines. Fruit extract of Gleditsia sinensis exhibited anti-cancer and telomerase inhibition effects on ESCC (esophageal squamous cell carcinoma) cell line (39). In human lung carcinoma cell line A549, aqueous extract of the Platycodon grandiflorum root represents the apoptotic events with the reduced telomerase activity and downregulation of Bcl2 expression (40).…”

Section: Discussionmentioning

confidence: 99%

Effect of Achillea wilhelmsii extract on expression of the human telomerase reverse transcriptase mRNA in the PC3 prostate cancer cell line

et al. 2017

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Abstract. Evidence has indicated that human telomerase reverse transcriptase (hTERT) was overexpressed in prostate cancer (PCa). Achillea wilhelmsii (AW) is a plant that has been traditionally used for its medicinal properties. The aim of current study was to evaluate the effects of AW extract on a PCa cell line. The cytotoxic activity of the hydroalcoholic extract of AW was studied on the PCa PC3 cell line using MTT assay. Flow cytometry was used to evaluate the effects of the extract on the apoptosis. The expression of hTERT mRNA was analyzed by the reverse transcription-quantitative polymerase chain reaction method. The ELISA method was used to measure the levels of telomerase enzyme. The hydroalcoholic AW extract demonstrated the appropriate inhibitory effect in 150 µg/ml concentration (IC 50 ) on PC3 cell line following 48 h treatment. Treatment of the PC3 cells with AW resulted in a significant increase in early and late apoptotic cells and a decrease in live cells (P<0.001), in a dose-dependent manner. Moreover, the early apoptotic cells were significantly higher than late apoptotic cells. The hTERT mRNA expression was decreased following 24 h treatment of AW extract, although it was not different between 2, 4, 8 and 12 h treatments or 24, 48 and 72 h treatments. In addition, the hTERT concentration was significantly decreased following 24 h treatment of AW extract with the marginal P-value. There was no significant difference regarding hTERT concentration between 2, 4, 8 and 12 h treatments or 24, 48 and 72 h treatments. The hydroalcoholic extract of AW induced potent antiproliferative and apoptotic effects in PC3 cell line, which could be explainable by its high potency to inhibit expression of the prominent oncogene hTERT in PCa. Therefore, targeting telomerase represents a promising strategy for PCa therapy, and AW may have considerable potential for development as a novel natural anticancer agent.

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“…Its crude extract showed anti-allergenic (13) and anti-inflammatory effects (14). However, scientific evidence remains unclear for support of the anti-tumor effect of the extract of Gleditsia sinensis thorns, although the fruit extract of Gleditsia sinensis has been credited with tumor growth inhibition in several cell lines (15,16). The present study is the first report of the anti-tumor effect of Gleditsia sinensis thorns on a relevant pathway between the cell cycle regulation and signaling pathways involved in growth inhibition and MMP regulation in human colon cancer HCT116 cells in vitro.…”

Section: Introductionmentioning

confidence: 99%

Inhibitory effects of the ethanol extract of Gleditsia sinensis thorns on human colon cancer HCT116 cells in vitro and in vivo

Lee

Cho²,

Kim³

et al. 2009

Oncol Rep

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Abstract. The thorns of Gleditsia sinensis have traditionally been used in the treatment of several diseases, which includes their use as anti-tumor agents, but there has been no scientific evidence of this anti-tumor effect. However, the present study has identified a novel mechanism for the anti-tumor effect of Gleditsia sinensis thorns in the treatment of colon cancer. Treatment with the ethanol extract of Gleditsia sinensis thorns (EEGS) resulted in significant growth inhibition together with G2/M-phase cell cycle arrest at a dose of 600 μg/ml (IC 50 ) in HCT116 cells. In addition, treatment with EEGS induced p27 expression and down-regulated expression of cyclins and cyclin-dependent kinases. Moreover, EEGS treatment induced phosphorylation of extracellular signal-regulated kinases (ERK), p38 MAP kinase and JNK (c-Jun N-terminal kinases). Among the pathways examined, only PD98059 (ERK-specific inhibitor) abolished EEGS-dependent p27 expression. Similarly, suppression of ERK function reversed EEGS-mediated cell proliferation inhibition and decreased cell cycle proteins. In addition, tumor necrosis factor-· (TNF-·)-induced matrix metalloproteinase-9 (MMP-9) expression was inhibited by EEGS treatment via decreased transcriptional activity of both activator protein-1 (AP-1) and nuclear factor-κB. Finally, EEGS treatment significantly reduced tumor sizes in HCT116 cell-xenografted tumor tissues, which was associated with the changed levels of ERK phosphorylation, p27 and MMP-9 expression. Overall, these results have identified a novel molecular mechanism for EEGS in the treatment of colon cancer and might provide a theoretical basis for the potential therapeutic use of EEGS in the treatment of malignancies.

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Inhibitory effects of Gleditsia sinensis fruit extract on telomerase activity and oncogenic expression in human esophageal squamous cell carcinoma

Cited by 18 publications

References 36 publications

Detection of regional DNA methylation using DNA-graphene affinity interactions

Detection of regional DNA methylation using DNA-graphene affinity interactions

Effect of Achillea wilhelmsii extract on expression of the human telomerase reverse transcriptase mRNA in the PC3 prostate cancer cell line

Inhibitory effects of the ethanol extract of Gleditsia sinensis thorns on human colon cancer HCT116 cells in vitro and in vivo

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