Inhibitory effects of copper on bacteria related to the free ion concentration

Abstract

Cu(2+) ion determinations were carried out in complex and in inorganic salts-glycerol media, to which increasing amounts of Cu(II) had been added, with the ion-specific Cu(II)-Selectrode. Likewise, complexing capacity of bacterial suspensions was estimated by titration with CuSO4.Copper-sensitive bacteria, e.g.,Klebsiella aerogenes, were inhibited in their growth and survival in the range of 10(-8)-10(-6) M Cu(2+) ion concentrations. In copper-buffered complex media, high copper loads could be tolerated, as growth proceeded with most of the copper bound to medium components. In low-complexing mineral salts media, in which high Cu(2+) ion concentrations exist at low copper loads, there was competition of Cu(2+) for binding sites of the cells. Total allowed copper was then determined by the ratio of copper to biomass.Copper-resistant bacteria could be isolated from a stock solution of CuSO4, containing 100 ppm Cu(II). They were of thePseudomonas type and showed a much higher tolerance towards Cu(2+), up to 10(-3) M.

Impacts of Hydrogen Peroxide and Copper Sulfate on the Control of Microcystis aeruginosa and MC-LR and the Inhibition of MC-LR Degrading Bacterium Bacillus sp.

“…In addition, Zevenhuizen et al (1979) The change in pH was monitored for the experiments at copper sulfate pentahydrate doses of 1 mg·L −1 , 1.5 mg·L −1 , and 2 mg·L −1 , and the results are shown in Figure S6 of the Supplementary Information. The pHs were found to reduce from an initial 7.4 to 6.2 at the end of the experiments for all the studied cases.…”

First Report of Copper-resistant Xanthomonas citri pv. citri Pathotype A Causing Asiatic Citrus Canker in Réunion, France

“…A total of 115 strains produced the expected amplicon whereas others (including all historical strains) and the negative control (H 2 O) did not. Phenotypic tests were carried out on low-complexing mineral salts casitone-yeast extract-glycerol-agar (CYE) plates (Zevenhuizen et al 1979) flooded with bacterial suspensions (≈5 × 10 6 cfu ml -1 ) in sterile tris buffer (pH 7.2), allowed to dry for 15 min., inoculated with a sterile …”

Copper-Induced Inhibition of Growth of Desulfovibrio desulfuricans G20: Assessment of Its Toxicity and Correlation with Those of Zinc and Lead

“…Both in laboratory studies and in field studies, it has been shown that the toxicity of a given metal depends on species and chemical properties as well as environmental factors (e.g., adsorption to solid surfaces, complexation, or precipitation) (22,26,34,43,57). Heavy metal toxicity is also known to interfere with important microbial processes including aerobic and anaerobic degradation of organic matter (3,28,41).…”