Copper-Induced Inhibition of Growth of
            <i>Desulfovibrio desulfuricans</i>
            G20: Assessment of Its Toxicity and Correlation with Those of Zinc and Lead

Sani, Rajesh K.; Peyton, Brent M.; Brown, Laura T.

doi:10.1128/aem.67.10.4765-4772.2001

Cited by 186 publications

(120 citation statements)

References 55 publications

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“…This observation suggests a remarkable potential for application of these fungi in chromium biotreatment. Sani et al [62] had reported similar observation with Desulfovibrio desulfuricans where toxic concentration of the heavy metal ions, Cu(II), Pb(II) and Zn(II) utterly inhibited growth, but did not kill the cells of the organism. Although Ezzouhri et al [63] reported 10 -15 mM of Cr(VI) as the Minimum Inhibitory Concentration (MIC) for Aspergillus species, the results obtained however revealed that the response of the isolates to heavy metals depended on the metal tested, its concentration in the medium and on the isolate considered.…”

Section: Regeneration Of Fungal Cells After Initial Exposure To Cr(vi)supporting

confidence: 48%

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Uptake and Reduction of Hexavalent Chromium by Aspergillus Niger and Aspergillus Parasiticus

Shugaba¹,

Buba²,

Bg³

et al. 2012

J Pet Environ Biotechnol

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The uptake and reduction of Cr(VI) by Aspergillus niger and A. parasiticus was studied in this journal. After 96 hours of growth, the culture solutions spiked with an initial dichromate concentration of 20 mg/l, were completely decolorized and had residual Cr(VI) concentrations of only 0.74 ± 0.55 and 1.69 ± 0.29 mg/l in A. niger and A. parasiticus cultures representing Cr(VI) removal of 96.3% and 91.6%, respectively. In the A. niger culture, significantly (P < 0.01) lower Cr(VI) concentrations were observed within 72 hours of growth compared to those of A. parasiticus, but in both cultures complete removal was almost achieved by 144 hours of growth. The rate of Cr(VI) removal was 0.21 ± 0.09 mgl -1 hr -1 and 0.20 ± 0.07 mgl -1 hr -1 for A. niger and A. parasiticus, respectively. Cellular concentrations of Cr(VI) in the two fungi increased significantly (P < 0.05 -0.001) with increasing concentrations of the dichromate treatments. Although tannic acid as sole source of carbon and energy gave significantly lower Cr(VI) removal than glucose (P < 0.001) and acetate (P < 0.01), it supported the removal of about 85.0% and 68.8% of the metal ion by A. niger and A. parasiticus, respectively. The active mycelia of both fungi showed significantly (P < 0.001) higher Cr(VI) removal than inactivated mycelia after incubation at 30°C for 72 hours. Incubation of cell -free extracts of both fungi with NADH at 30°C for 2 hours showed Cr(VI) reduction of 68.0% and 55.5% for A. niger and A. parasiticus, respectively. These findings suggest that uptake and metabolic reduction may be the process by which the two fungi are able to tolerate the toxic effects of hexavalent chromium. However, Cr removal via uptake by the two fungal biomass was observed to be in the range of 0.5 -1.78% only, for all the concentrations applied, which is insignificant when compared with the initial Cr concentration in the culture medium. The results obtained through this investigation indicate the possibility of treating waste effluents containing hexavalent chromium using Aspergillus niger and A. parasiticus.

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Section: Regeneration Of Fungal Cells After Initial Exposure To Cr(vi)supporting

confidence: 48%

“…Hexavalent chromium is highly toxic to a lot of microorganisms, and like other heavy metal ions such as Cu(II), Pb(II), Zn(II), etc. it may cause structural damage in cells and consequently a permanent effect on cell growth [34,62].…”

Section: Regeneration Of Fungal Cells After Initial Exposure To Cr(vi)mentioning

confidence: 99%

Uptake and Reduction of Hexavalent Chromium by Aspergillus Niger and Aspergillus Parasiticus

Shugaba¹,

Buba²,

Bg³

et al. 2012

J Pet Environ Biotechnol

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“…For the conditions of copper sulfate doses <0.5 mg·L −1 , the mortality rate constants were all less than 10 −3 h −1 , suggesting that the effect on the studied bacterium is negligible. Sani et al (2001) [63] reported an IC50 of 13.3 µM copper (3.3 mg copper sulfate pentahydrate·L −1 ) to sulfate-reducing bacteria (SRB) Desulfovibrio desulfuricans G20. When a higher dose was applied, 30 µM copper (7.4 mg copper sulfate pentahydrate·L −1 ), 100% of the SRB were killed in 25 h and no bacteria were detected after 384 h of incubation.…”

Section: Resultsmentioning

confidence: 99%

Impacts of Hydrogen Peroxide and Copper Sulfate on the Control of Microcystis aeruginosa and MC-LR and the Inhibition of MC-LR Degrading Bacterium Bacillus sp.

Kansole

Lin

2017

Water

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Abstract:Laboratory batch experiments were carried out to evaluate the impacts of H 2 O 2 and copper sulfate on M. aeruginosa PCC7820, microcystin-LR (MC-LR) and its degrading bacteria Bacillus sp., previously isolated from Hulupi Lake in Taiwan. The study shows that 3 mg·L −1 hydrogen peroxide removed only 9% M. aeruginosa within seven days of exposure, from an initial cell concentration of 2 × 10 6 cells/mL. With copper sulfate, a concentration of 2 mg·L −1 removed 99% M. aeruginosa cells, but showed negligible efficacy in removing 0.05 mg·L −1 MC-LR. At a higher dosage, 20 mg·L −1 H 2 O 2 led to 40% and 95% removal, respectively for MC-LR and M. aeruginosa cells. Copper sulfate and H 2 O 2 were both lethal to Bacillus sp. population, with mortality rate constants of k = 0.04 h −1 and 0.03 h −1 under 1 mg·L −1 copper sulfate and 5 mg·L −1 H 2 O 2 , respectively. H 2 O 2 is competitive in terms of cost, with a capability of degrading organic compounds with the assistance of ultraviolet (UV) light, and it may be considered as an alternative algaecide to copper sulfate in reservoirs for algae growth control.

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“…44) Bacterial growth was estimated by measurement of total cellular protein using the Bradford method. 11,[45][46][47] Cell viability also served as a visual check for contamination.…”

Section: Discussionmentioning

confidence: 99%

Chromium(VI) Resistance and Extracellular Polysaccharide (EPS) Synthesis by Pseudomonas, Stenotrophomonas and Methylobacterium Strains

2008

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Microorganisms have great value in research, for multiple purposes during the last several decades. Resistance of various strains of microorganisms against chromium and the production of the extracellular polysaccharide (EPS) had been experimented and reveals the fact that the most of the strains (Pseudomonas, Stenotrophomonas, Methylobacterium) which produced high concentration of EPS shown resistance to Cr(VI). The most resistant Methylobacterium mesophilium MU141 (435 mg/L) secreted higher EPS than the rest of the strain. The toxic effect of Cr(VI) on cell viability and total cell protein of M. mesophilium MU 141 was treated by different concentration of Cr(VI), ranged from 15 and 35 ppm for 24 h. Hence, concentration of Cr(VI) can affect the EPS production at certain spectrum. Therefore, the result discloses the correlation between Cr(VI) resistance and EPS production among Pseudomonas, Stenotrophomonas, Methylobacterium strains.

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Copper-Induced Inhibition of Growth of Desulfovibrio desulfuricans G20: Assessment of Its Toxicity and Correlation with Those of Zinc and Lead

Abstract: The toxicity of copper [Cu(II)] to sulfate-reducing bacteria (SRB) was studied by using Desulfovibrio desulfuricans G20 in a medium (MTM) developed specifically to test metal toxicity to SRB (R. K. Sani, G.

Cited by 186 publications

References 55 publications

Uptake and Reduction of Hexavalent Chromium by Aspergillus Niger and Aspergillus Parasiticus

Uptake and Reduction of Hexavalent Chromium by Aspergillus Niger and Aspergillus Parasiticus

Impacts of Hydrogen Peroxide and Copper Sulfate on the Control of Microcystis aeruginosa and MC-LR and the Inhibition of MC-LR Degrading Bacterium Bacillus sp.

Chromium(VI) Resistance and Extracellular Polysaccharide (EPS) Synthesis by Pseudomonas, Stenotrophomonas and Methylobacterium Strains

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