Inhibitory effects of cold atmospheric plasma on the growth, ergosterol biosynthesis, and keratinase activity in Trichophyton rubrum

Shapourzadeh, Atena; Rahimi-Verki, Neda; Atyabi, Seyed-Mohammad; Shams-Ghahfarokhi, Masoomeh; Jahanshiri, Zahra; Irani, Shiva; Razzaghi-Abyaneh, Mehdi

doi:10.1016/j.abb.2016.07.012

Cited by 21 publications

(17 citation statements)

References 31 publications

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“… Shapourzadeh et al (2016) reported that cold atmospheric plasma (CAP) inhibited the growth of T. rubrum while moderately increasing keratinase activity, which probably occurred as compensation by the fungus for CAP-mediated fungal growth inhibition. However, we found that IPL inhibited the growth of T. rubrum and decreased fungal keratinase activity.…”

Section: Discussionmentioning

confidence: 99%

The Role of NADPH Oxidase in the Inhibition of Trichophyton rubrum by 420-nm Intense Pulsed Light

Huang

Chen

et al. 2018

Front. Microbiol.

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Objectives: To evaluate the effect of intense pulsed light (IPL) on Trichophyton rubrum and investigate its mechanism of action.Methods: The viability of fungi treated with IPL alone and with IPL combined with an NADPH oxidase inhibitor (DPI) pretreatment was determined by MTT assays. The reactive oxygen species (ROS) were quantified with a DCFH-DA fluorescent probe. Malondialdehyde (MDA) content and superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) activities were determined by commercial kits. The transcription of the Nox gene was quantified using quantitative real-time PCR (qRT-PCR) analysis, and micromorphology was observed using scanning electron microscopy (SEM). In addition, fungal keratinase activity was detected by measuring dye release from keratin azure.Results: The growth declined with statistical significance after 6 h of treatment (P < 0.001). The ROS and MDA content increased after IPL treatment, whereas the SOD and GSH-Px activity decreased. Nox gene expression was upregulated, and the micromorphology was damaged. Keratinase activity decreased. Fungi that received DPI pretreatment exhibited contrasting outcomes.Conclusion: We found that 420-nm IPL significantly inhibited the growth and pathogenicity of T. rubrum in vitro. A suggested mechanism involves Nox as a factor that mediates 420-nm IPL-induced oxidative damage of T. rubrum.

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Section: Discussionmentioning

confidence: 99%

The Role of NADPH Oxidase in the Inhibition of Trichophyton rubrum by 420-nm Intense Pulsed Light

Huang

Chen

et al. 2018

Front. Microbiol.

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“…Most studies on the disinfection effects of NTP were devoted to bacteria, but attempts to inactivate fungi both in vitro and in vivo have also been reported [26][27][28][29][30][31]. Thus, this issue is sufficiently and extensively evaluated, and the results show the possibility of effective inactivation of fungi by NTP.…”

Section: Introductionmentioning

confidence: 99%

Inactivation of Dermatophytes Causing Onychomycosis and Its Therapy Using Non-Thermal Plasma

Lux

Dobiáš

Kuklová

et al. 2020

JoF

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Onychomycosis is one of the most common nail disorders. Its current treatment is not satisfactorily effective and often causes adverse side effects. This study aims to determine the optimal conditions for non-thermal plasma (NTP) inactivation of the most common dermatophytes in vitro and to apply it in patient`s therapy. The in vitro exposure to NTP produced by negative DC corona discharge caused full inactivation of Trichophyton spp. if applied during the early growth phases. This effect decreased to negligible inactivation with the exposure applied six days after inoculation. In a group of 40 patients with onychomycosis, NTP therapy was combined with nail plate abrasion and refreshment (NPAR) or treatment with antimycotics. The cohort included 17 patients treated with NPAR combined with NTP, 11 patients treated with antimycotics and NTP, and 12 patients treated with NPAR alone. The combination of NPAR and NTP resulted in clinical cure in more than 70% of patients. The synergistic effect of NPAR and NTP caused 85.7% improvement of mycological cure confirmed by negative microscopy and culture of the affected nail plate. We conclude that NTP can significantly improve the treatment of onychomycosis.

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“…Cold atmospheric plasma jet application. The plasma device used in this study was a Dielectric Barrier Discharge (DBD) that consisted of helium gas with a power supply of 10 kV pulses, a frequency of 6 kHz, and an external electrode as the earth [11]. The applied voltage to the electrode ionized the inlet gas fed into the tube, and the generated plasma was emitted out from the end of the tube.…”

Section: Methodsmentioning

confidence: 99%

Bioactivity Determination of Recombinant lysostaphin Immobilized on Glass Surfaces Modified by Cold Atmospheric Plasma on Staphylococcus aureus

Ehsani

Fahmide

Norouzian

et al. 2019

JoMMID

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Introduction: Staphylococcus aureus is a source of nosocomial infections and one of the significant concerns in patients with indwelling devices. Lysostaphin is a bacterially produced endopeptidase with a unique activity on S. aureus. Plasma, the fourth state of the material, consists of charged ions, free electrons, and activated neutral species. Biomedical applications of cold plasma are rapidly growing due to its capacity to treat heat-sensitive objects such as polymeric materials and biological samples. It activates surfaces by etching them to stabilize proteins. The direct effect of cold atmospheric plasma on the eradication of microorganisms have been investigated. However, there is no report on immobilizing antibiotic agents. Methods: In this study, the lysostaphin protein was expressed and purified using Ni-NTA column, then the purified enzyme was immobilized on glass surfaces pretreated with cold atmospheric plasma for 150 s, 200 s, and 300 s. The antimicrobial activity of immobilized lysostaphin on S. aureus was approved by in vitro analysis. Results: The 300 s plasma treatment confirmed to be the best time arrangement for more lysostaphin immobilization, shown by Atomic Force Microscopy. Conclusion: Our results showed that passive adsorption to the treated surface does not affect the structure and subsequent antimicrobial function of the recombinant protein compared to the standard proteins.

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Inhibitory effects of cold atmospheric plasma on the growth, ergosterol biosynthesis, and keratinase activity in Trichophyton rubrum

Cited by 21 publications

References 31 publications

The Role of NADPH Oxidase in the Inhibition of Trichophyton rubrum by 420-nm Intense Pulsed Light

The Role of NADPH Oxidase in the Inhibition of Trichophyton rubrum by 420-nm Intense Pulsed Light

Inactivation of Dermatophytes Causing Onychomycosis and Its Therapy Using Non-Thermal Plasma

Bioactivity Determination of Recombinant lysostaphin Immobilized on Glass Surfaces Modified by Cold Atmospheric Plasma on Staphylococcus aureus

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