Inhibitory Effects of Active Compounds Isolated from Safflower (Carthamus tinctorius L.) Seeds for Melanogenesis

Roh, Jung Seop; Han, Ji Young; Kim, Jung Han; Hwang, Jae Kwan

doi:10.1248/bpb.27.1976

Cited by 175 publications

(85 citation statements)

References 11 publications

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“…23) A 105 ml aliquot of 100 mM phosphate buffer (pH 6.8), 10 ml of various concentrations of samples and 20 ml of 200 U/ml mushroom tyrosinase were mixed and incubated at room temperature. After a 10-minute incubation, 65 ml of 2.5 mM L-DOPA was added and the mixture incubated for another 10 min at room temperature.…”

Section: Methodsmentioning

confidence: 99%

Combination of Amino Acids Reduces Pigmentation in B16F0 Melanoma Cells

Ishikawa¹,

Kawase²,

Ishii³

2007

Biological & Pharmaceutical Bulletin

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Section: Methodsmentioning

confidence: 99%

Combination of Amino Acids Reduces Pigmentation in B16F0 Melanoma Cells

Ishikawa¹,

Kawase²,

Ishii³

2007

Biological & Pharmaceutical Bulletin

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“…Therefore, search of medicinal plants possessing high efficacy with low toxicity has been conducted for cosmetic and medicinal purposes [5].…”

Section: Introductionmentioning

confidence: 99%

Anti-melanogenesis Active Constituents from the Extracts of Carpinus turczaninowii Leaves

강지미¹,

김정은²,

Lee³

2017

Journal of the Society of Cosmetic Scientists of Korea

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Melanin synthesis inhibition activities were investigated for the extracts prepared from the leaves of Carpinus turczaninowii (C. turczaninowii) by using B16F10 melanoma cells. As a result, the ethanol extract (100 µg/mL) showed 72.2% inhibition activities without cell toxicities in MTT assays. For the solvent fractions (n-hexane, ethyl acetate, n-butanol, water), the most potent activities were observed at the ethyl acetate fraction. To isolate the active constituents, the ethyl acetate fraction was further purified to afford four compounds; ethyl gallate (1), quercetin rhamnose (2), kaempferol rhamnose (3) and quercetin galloylrhamnose (4). The identification of the isolates was made by spectroscopic data including NMR spectra, and all of the compounds 1-4 were isolated for the first time from the leaves of C. turczaninowii. Anti-melanogenesis activities were studied for the isolates 1-4, and the compound 4 was determined to decrease the melanin synthesis dose-dependently without causing cell toxicities. ELISA measurement indicated that the isolate 4 decreased the contents of cell tyrosinase, a critical enzyme in melanogenesis. Based on these results, the extracts of C. turczaninowii were found to be applicable as whitening ingredients in cosmetic formulations.

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“…Although various biological activities of C. tinctorius flowers and leaves have been reported (Sakamura et al 1978), there have been few investigations of their inhibitory activity with respect to inflammation. Roh et al report is pretty much the only research paper about a whitening effect using safflower (C. tinctorius L.) seeds (Roh et al 2004). We previously reported that the partially purified ethyl acetate (EtOAc) soluble fraction of C. tinctorius seeds showed inhibition of cytokine, iNOS and COX-2 expression (Kim et al 2013).…”

Section: Introductionmentioning

confidence: 99%

Serotonins of safflower seeds play a key role in anti-inflammatory effect in lipopolysaccharide-stimulated RAW 264.7 macrophages

et al. 2015

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Serotonins of safflower seeds play a key role in anti-inflammatory effect in lipopolysaccharide-stimulated RAW 264.7 macrophages Dong-Hee Kim ･ Yong-Sun Moon ･ Tae-Soon Park ･ Jun-Ho Son Abstract Safflower (Carthamus tinctorius) seeds are wellknown traditional oriental medicines that have long been used for the remedies of blood stasis and bone formation in east Asia. In this study, ethyl acetate (EtOAc) was used for extraction of the main chemical compounds from C. tinctorius seeds. Four major compounds were identified, acacetin, cosmosiin, N-feruloyl serotonin and N-(p-coumaroyl) serotonin. Each compound was evaluated for its inhibitory activity against the inflammatory process of macrophages. All compounds significantly inhibited production of lipopolysaccharide (LPS)-stimulated nitric oxide (NO) and pro-inflammatory cytokines. The protein levels of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) were dramatically decreased by serotonins in a dose-dependent manner in LPS-stimulated RAW 264.7 macrophages. These results suggest that serotonin derivatives from safflower seeds may reduce inflammationrelated diseases.

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Inhibitory Effects of Active Compounds Isolated from Safflower (Carthamus tinctorius L.) Seeds for Melanogenesis

Cited by 175 publications

References 11 publications

Combination of Amino Acids Reduces Pigmentation in B16F0 Melanoma Cells

Combination of Amino Acids Reduces Pigmentation in B16F0 Melanoma Cells

Anti-melanogenesis Active Constituents from the Extracts of Carpinus turczaninowii Leaves

Serotonins of safflower seeds play a key role in anti-inflammatory effect in lipopolysaccharide-stimulated RAW 264.7 macrophages

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