Inhibitory Effects and Anti-Invasive Activities of Trehalose Liposomes on the Proliferation of Lung Carcinoma Cells

Abstract

Inhibitory effects and anti-invasive activities of trehalose liposomes (DMTreC14) composed of L-α-dimyristoylphosphatidylcholine and α-D-glycopyranosyl-α-D-glucopiranoside monomyristate (TreC14) on the proliferation of human non-small cell lung carcinoma (A549) cells were examined in vitro. DMTreC14 with a hydrodynamic diameter less than 100 nm, were preserved for 4 weeks. The inhibitory effects of DMTreC14 on the proliferation of A549 cells accompanied with apoptosis were obtained. Enhancement of the accumulation of DMTreC14 into A549 membranes was observed. An increase in cellular membrane fluidity of A549 cell treated with DMTreC14 was observed on the basis of fluorescence depolarization method. DMTreC14 caused apoptosis for A549 cells through the activation of caspases and mitochondrial pathway. The anti-migration effects of DMTreC14 for A549 cells were observed through the inhibition of filopodia formation. The anti-invasion effects with the reduction of MMP-14 of DMTreC14 for A549 cells were obtained.

Apoptotic and Anti-invasive Effects of Hybrid Liposomes without Drugs against Human Breast Cancer Cells In Vitro

“…The same we have employed in vitro invasion assay in Matrigel invasion chamber ( Figure 5) [16,[20][21][22]. Reduced rate of invasiveness was evident in the present study after HL treatment in dose dependent manner.…”

“…The antimigration effects of HL were scrutinized at low concentrations (200 μM) without affecting growth-inhibition below IC 50 values (200 μM). Additionally, pseudopodium development on the exterior of tumour cells has a chief responsibility in the invasion and migration into the neighbouring tissue [18][19][20]. The suppression actions of HL on the pseudopodium formation of MCF-7 cells were scrutinised by using confocal laser microscopy ( Figure 4).…”

Trehalose Liposomes Suppress the Growth of Tumors on Human Lung Carcinoma-bearing Mice by Induction of Apoptosis In Vivo

“…Trehalose liposomes inhibited the growth of colon and gastric cancer, hepatic carcinoma and leukemia (8)(9)(10). Inhibitory effects and anti-invasive activities of trehalose liposomes against lung carcinoma have been obtained in vitro (11 …”

Inhibitory Effects of Trehalose Liposomes against Breast Cancer Cells Leading to Apoptosis in vitro

“…Inhibitory effects of trehalose liposomes composed of phosphatidylcholine and trehalose surfactants, on colon cancer, gastric cancer, hepatic cancer, leukemia, and lung carcinoma cells have been reported [5][6][7][8][9]. However, the effect of treatment with trehalose containing liposomes, on the growth of breast cancer cells has not yet been investigated.…”