Inhibitory Effect on Arginase and Total Phenolic Content Determination of Extracts from Different parts of Melastoma malabathricum L.

Abstract

The part of the plant were collected and cleaned, dried at room temperature, and then powdered and stored in an air tight glass container. 50 g of each plant part was extracted by maceration with 500 mL of 70% ethanol ABSTRACTBackground: Some phenolic compounds are potential source for arginase inhibitor that can be used in management of disease associated with endothelial dysfunction. Objective: The aim of this study was to investigate the inhibitory effect on arginase and to determine total phenolic content of extracts from different parts of Melastoma malabathricum L. Methods: Each part were extracted with 70% ethanol by maceration. The extracts were mixed with bovine liver arginase and L-arginine as a substrate. The inhibitory effect of extracts were determined in vitro, by measuring its absorbance on 430 nm. Total phenolic content were determined with Folin-Ciocalteu 25% on 750 nm. Results: The 70% ethanol extract of M. malabathricum leaves at 100 µg/mL was found to have highest inhibition (81.26 ± 5.27 %) followed by flower 73.39 ± 9.39%, fruit 67.63 ± 7.61 and stem 61.61 ± 4.56%. The IC 50 value of the most active extract was 62.43 µg/mL while the IC 50 value of N-hidroksi-nor-L-arginin (nor-NOHA) acetate, an arginase inhibitor was found to be 3.91 µg/mL. Total phenolic content of 70% ethanolic extracts of M. malabathricum leaves, flower, fruit and stem was 15.9, 20.7, 6.44, and 6.29%. Conclusion: The 70% ethanol extract of M. malabathricum leaves exhibited the highest inhibition on arginase but highest total phenolic content was from flower part. Pearson correlation test showed no correlation p>0.05 between arginase inhibition and total phenolic content of the samples.

Arginase inhibitory and antioxidant activities in Syzygium cumini (L.) Skeels leaves extracts collected from three different locations of java

“…Arginase inhibitory activity was determined using arginase enzymatic assay protocol (Sigma Aldrich, Singapore) and urea assay kit (Abnova Corporation, Taiwan) as reported by previous authors 19 with slight modification. Using 96-well microplate, 10 µL of each extract solution at the same concentration was mixed with 15 µL of arginase solution (1 U/mL) and 20 µL L-arginine (Sigma, Singapore) 380 mM.…”

Syzygium Cumini (L.) Skeels Leaf Extract Fractions as Arginase Inhibitors and the Effects of Tannins on Their Activity

“…Enzyme inhibition activity was determined using arginase enzymatic assay (Sigma-Aldrich, Singapore) and urea determination assay kits (Abnova Corporation, Taiwan), as described elsewhere [9]. The test (10 µL), arginase 1 U/mL (15 µL), and L-arginine 380 mM (20 µL) solutions were mixed in 96-well microplates and incubated at 37°C for 30 min.…”

“…Total phenolic content of the active fractions that inhibited arginase was determined using the Folin-Ciocalteu method [9]. In a 96-well microplate, 100 µL of diluted Folin-Ciocalteu reagent (1:4) was added to 25 µL of either a fraction or standard solution.…”

“…Studies have shown arginase inhibitory activities of plant substances or extracts. Some examples include Theobroma cacao flavonol mixture, Caesalpinia sappan heartwood ethyl acetate extract, Korean red ginseng root extract, Scutellaria indica flavanones, and hydroalcoholic extract from Melastoma malabathricum leaves [5][6][7][8][9]. Some natural compounds have also been reported to show arginase inhibitory activity in vitro, such as chlorogenic acid, piceatannol, resveratrol, (−)-epicatechin, taxifolin, quercetin, and caffeic acid [10].…”

Arginase Inhibitory, Antioxidant Activity, Total Phenolic Content and Total Flavonoid Content of Ethyl Acetate Extract of Caesalpiniaturtuosa Roxb Stem Bark

“…Endothelial dysfunction is a pathogenic factor of many diseases such as diabetes, arthritis, and hypercholesterolemia. [1][2][3] Endothelial cell dysfunction also contributes to cardiovascular disease development like atherosclerosis, hypertension, myocardial infarction, and stroke. [4][5][6] Major contributor for endothelial dysfunction is Nitric oxide (NO) bioavailability decreasing.…”