Inhibitory Effect on Angiotensin-converting Enzyme (ACE) and Optimization for Production of Ovotransferrin Hydrolysates

Abstract

Angiotensin-converting enzyme (ACE) inhibitory activity and production optimization of ovotransferrin hydrolysates were studied. Ovotransferrin was hydrolyzed by several enzymes (protamex, alcalase, trypsin, pepsin, neutrase, and flavorzyme) and acid (0.03 N HCl). Ovotransferrin hydrolysate reduced ACE activity by 60.2%, 55.8%, and 42.6% when treated with trypsin, acid, and pepsin, respectively. Trypsin was selected for production of peptide having maximum ACE inhibitory effect, which was greatest with 7 h hydrolysis. Central composite design determined that optimum composition of ACE inhibitory substances using substrate concentration of 20-35%, temperature of 35-55

Enzymatic Hydrolysis of Ovotransferrin and the Functional Properties of Its Hydrolysates

“…Several peptides from egg proteins have been studied for their biological activities. The bioactive peptides in the native ovotransferrin were produced using various methods, including acid hydrolysis (Ibrahim et al, 2000;Lee et al, 2010), autocleaving under reduced conditions (Ibrahim and Kiyono, 2009;Moon et al, 2014), and enzymatic hydrolysis (Huang et al, 2010;Shen et al, 2010;Moon et al, 2013;Wang et al, 2017;Ma et al, 2020). All the studies indicated that the functionality of ovotransferrin increased after the hydrolysis.…”

Novel Antioxidant Peptide Derived from the Ultrafiltrate of Ovomucin Hydrolysate

“…Hydrolysates of ovotransferrin have also been shown to exert an inhibitory effect on ACE. 14,15 The hydrolysate of ovalbumin also has an antihypertensive effect. 16 Among the various bioactive peptides derived from food proteins, those having antioxidant properties are important because free radicals produced by oxidative reactions might result in different disorders such as diabetes mellitus, arthrosclerosis, cancer, and coronary heart disease, 17−19 in addition to causing allergies and aging 20−22 due to damage to cellular components.…”

“…Other studies on bioactive peptides were reported by Majumder and Wu, , who isolated peptides exhibiting inhibitory activity against angiotensin I-converting enzyme (ACE) from the simulated gastrointestinal digestion of proteins contained in cooked egg. Hydrolysates of ovotransferrin have also been shown to exert an inhibitory effect on ACE. , The hydrolysate of ovalbumin also has an antihypertensive effect …”

Functional properties of ovotransferrin from chicken egg white and its derived peptides: a review

“…Various approaches to hydrolyze ovotransferrin, including chemical and enzymatic methods, have been reported (Ibrahim et al, 2000 ; Kim et al, 2012 ; Lee et al, 2010 ; Moon et al, 2013 ). Among the reported methods of hydrolyzing ovotransferrin, enzymatic hydrolysis was more widely used than other methods.…”

“…Moon et al ( 2017 ) investigated the ACE-inhibitory activity of promod 278P hydrolysates of ovotransferrin and reported that the hydrolyzed products exhibited a strong ACE-inhibitory activity. Lee et al ( 2010 ) reported that ovotransferrin hydrolysates produced with trypsin, acid, and pepsin reduced the activity of ACE by 60.2%, 55.8%, and 42.6%, respectively. Also, the ACE-inhibitory peptide (Lys-Val-Arg-Glu-Gly-Thr-Thr-Tyr) of ovotransferrin significantly reduced the blood pressure in spontaneously hypertensive mice (Lee et al, 2006 ).…”