Novel Antioxidant Peptide Derived from the Ultrafiltrate of Ovomucin Hydrolysate

Abstract

The techno-functional properties of ovomucin as a gel-forming agent and its biological properties are well-known. The aim of the present study was to investigate antioxidant activity in ovomucin hydrolysate using radical scavenging assays. Electrophoresis showed that ovomucin isolated from whole egg was well separated. Ovomucin hydrolysis was carried out using microbial protease according to different incubation times. These ovomucin hydrolysates exhibited 85% antioxidant activity as measured by the 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) assay after a 2 h incubation with protease and retained 90% activity until 24 h. At an incubation time of 4 h, the activity of ovomucin hydrolysates reached approximately 90%, corresponding to 115 μM gallic acid equivalent, regardless of the proteases used. The partially purified fraction of the hydrolysate by ultrafiltration and reverse-phase high-performance liquid chromatography was collected and then analyzed by liquid chromatography electrospray ionization mass spectrometry. Two peptides, LDEPDPL and NIQTDDFRT, in this fraction were identified. The antioxidant activities of these two synthesized peptides were measured to be 51.8 and 24.7% by the 2,2-diphenyl-1-picrylhydrazyl assay.

High Fischer ratio oligopeptides determination from Antartic krill: Preparation, peptides profiles, and in vitro antioxidant activity

“…Previous reports indicated that acidic amino acids played a critical role in hydroxyl radical scavenging activities of peptides, which is related to the carboxyl and amino groups in their side chains (Pan et al, ; Udenigwe & Aluko, ). Similar results were also reported by Chang et al who found that acidic (glutamic acid and aspartic acid) amino acid residues in the sequences of NTDGSTDYGILQINSR and LDEPDPLI were critical for their antioxidant activities (Chang et al, ). Hence, high content of glutamic acid and aspartic acid in HFP may be a favorable factor for its antioxidant activities.…”

Antioxidant Peptides from the Protein Hydrolysate of Monkfish (Lophius litulon) Muscle: Purification, Identification, and Cytoprotective Function on HepG2 Cells Damage by H2O2

“…Met residue acted as a reactive site in the PMRGGGGYHY, WMFDW, WMGPY, and EMGPA through formatting a sulfoxide structure to scavenge free radicals [18,56]. Pro residue can help antioxidant peptides, such as LDEPDPL, WMGPY and EMGPA, scavenge ROS because of the pyrrolidine ring [18,57]. Furthermore, aromatic groups of Trp, Phe, and Tyr residues can scavenge free radicals by providing protons [55][56][57][58].…”

Practical problems when using ABTS assay to assess the radical-scavenging activity of peptides: Importance of controlling reaction pH and time

“…A longer incubation period of 15 or 30 min has also been adapted by some authors (Chang et al, 2013;Gómez-Ruiz, López-Expósito, Pihlanto, Ramos, & Recio, 2008). There is a lack of consensus about the optimal incubation time.…”