Inhibitory Effect of Topical Cartilage Acellular Matrix Suspension Treatment on Neovascularization in a Rabbit Corneal Model

Yun, Hee-Woong; Choi, Byung Hyune; Park, Do Young; Jin, Long Hao; Min, Byoung‐Hyun

doi:10.1007/s13770-020-00275-3

Cited by 8 publications

(12 citation statements)

References 50 publications

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“…Various types of dECM bio-inks have been introduced for potential applications in artificial tissue regeneration. 5 – 10 In dECM bio-ink development, detergents are commonly used in the decellularization process and are the greatest determinants of the bio-ink properties. 16 – 18 , 20 – 22 Despite various studies on dECM bio-inks, analyses of the effectiveness of different detergents are lacking.…”

Section: Discussionmentioning

confidence: 99%

“…Various types of animal tissue-derived dECM bio-inks have been introduced. 4 – 7 Pati et al 8 reported that dECM bio-inks derived from the porcine heart, cartilage, and adipose tissue exhibit excellent performance in tissue-specific differentiation. Yi et al 9 introduced a tumor model printed with glioblastoma-derived dECM bio-ink that produces a patient-specific drug response.…”

Section: Introductionmentioning

confidence: 99%

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Effect of detergent type on the performance of liver decellularized extracellular matrix-based bio-inks

2021

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Decellularized extracellular matrix-based bio-inks (dECM bio-inks) for bioprinting technology have recently gained attention owing to their excellent ability to confer tissue-specific functions and 3D-printing capability. Although decellularization has led to a major advancement in bio-ink development, the effects of detergent type, the most important factor in decellularization, are still unclear. In this study, the effects of various detergent types on bio-ink performance were investigated. Porcine liver-derived dECM bio-inks prepared using widely used detergents, including sodium dodecyl sulfate (SDS), sodium deoxycholate (SDC), Triton X-100 (TX), and TX with ammonium hydroxide (TXA), were characterized in detail. SDS and SDC severely damaged glycosaminoglycan and elastin proteins, TX showed the lowest rate of decellularization, and TXA-based dECM bio-ink possessed the highest ECM content among all bio-inks. Differences in biochemical composition directly affected bio-ink performance, with TXA-dECM bio-ink showing the best performance with respect to gelation kinetics, intermolecular bonding, mechanical properties, and 2D/3D printability. More importantly, cytocompatibility tests using primary mouse hepatocytes also showed that the TXA-dECM bio-ink improved albumin secretion and cytochrome P450 activity by approximately 2.12- and 1.67-fold, respectively, compared with the observed values for other bio-inks. Our results indicate that the detergent type has a great influence on dECM damage and that the higher the dECM content, the better the performance of the bio-ink for 3D bioprinting.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Effect of detergent type on the performance of liver decellularized extracellular matrix-based bio-inks

2021

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“…CAM was prepared according to previously published methods (Kwon et al, 2013;Yun et al, 2020). We harvested articular cartilage from a 6-month-old pig leg using a surgical blade.…”

Section: Preparation Of Cx-cam Filmsmentioning

confidence: 99%

“…For example, a fragment or a powder form of cartilage ECM has been used as a biomaterial by itself or processed into a 3D construct for a chondro-inductive bio-scaffold in cartilage reconstruction (Chen et al, 2012;Sutherland et al, 2015). Cartilage ECM can also be formulated as a suspension for topical treatment or injection (Yun et al, 2020). The enzymatically digested cartilage ECM was easily converted into a thermosensitive hydrogel that can be used for drug depot or 3D printing.…”

Section: Introductionmentioning

confidence: 99%

“…Therefore, chemical modifications have been made to improve and adjust the physical properties according to the intended target (Adamiak & Sionkowska, 2020). In previous studies, film-type biomaterials made in solubilized cartilage acellular matrix (CAM) through electrospinning and glutaraldehyde (GA) crosslinking has been shown to inhibit postoperative adhesion between the peritoneal wall and the cecum in a rat model (Park et al, 2019;Yun et al, 2020). GA is commonly used as a crosslinker for collagen-based biomaterials, due to its excellent stability, ease of control, and costeffectiveness (Lee et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

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Effect of glutaraldehyde‐crosslinked cartilage acellular matrix film on anti‐adhesion and nerve regeneration in a rat sciatic nerve injury model

Shin

Yun

Park

et al. 2021

J Tissue Eng Regen Med

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Decellularized extra-cellular matrix (ECM) has been studied as an alternative to anti-adhesive biomaterials and cartilage acellular matrix (CAM) has been shown to inhibit postoperative adhesion in several organs. This study aimed to evaluate the suitability of glutaraldehyde (GA) crosslinked CAM-films as anti-adhesion barriers for peripheral nerve injury. The films were successfully fabricated and showed improved physical properties such as mechanical strength, swelling ratio, and lengthened degradation period while maintaining the microstructure and chemical composition after GA crosslinking. In the in vitro study of CAM-film, the dsDNA content met the recommended limit of decellularization and more than 70% of the major ECM components were preserved after decellularization. The adhesion and proliferation of seeded human umbilical vein endothelial cells and fibroblasts were significantly lower in CAM-film than in control, but similar with Seprafilm. However, the CAM-film extract did not show cytotoxicity. In the in vivo study, the peri-neural fibrosis was thicker, adhesion score higher, and peri-neural collagen fibers more abundant in the control group than in the CAM-film group. The total number of myelinated axons was significantly higher in the CAM-film group than in the control group. The inflammatory marker decreased with time in the CAM-film group compared to that in the control group, whereas the nerve regenerative marker expression was maintained. Moreover, the ankle angles at contracture and toe-off were higher in the CAM film-treated rats than in the control rats. GA-crosslinked CAM films may be used during peripheral nerve surgery to prevent peri-neural adhesion and enhance nerve functional recovery.

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Anti‐adhesive effect of chondrocyte‐derived extracellular matrix surface‐modified with poly‐L‐lysine

Song

Park

et al. 2021

J Tissue Eng Regen Med

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After an injury, soft tissue structures in the body undergo a natural healing process through specific phases of healing. Adhesions occur as abnormal attachments between tissues and organs through the formation of blood vessels and/or fibrinous adhesions during the regenerative repair process. In this study, we developed an adhesion-preventing membrane with an improved physical protection function by modifying the surface of chondrocyte-derived extracellular matrices (CECM) with anti-adhesion function. We attempted to change the negative charge of the CECM surface to neutral using poly-L-lysine (PLL) and investigated whether it blocked fibroblast adhesion to it and showed an improved anti-adhesion effect in animal models of tissue adhesion. The surface of the membrane was modified with PLL coating (PLL 10), which neutralized the surface charge. We confirmed that the surface characteristics except for the potential difference were maintained after the modification and tested cell attachment in vitro. Adhesion inhibition was identified in a peritoneal adhesion animal model at 1 week and in a subcutaneous adhesion model for 4 weeks. Neutralized CECM (N-CECM) suppressed fibroblast and endothelial cell adhesion in vitro and inhibited abdominal adhesions in vivo. The CECM appeared to actively inhibit the infiltration of endothelial cells into the injured site, thereby suppressing adhesion formation, which differed from conventional adhesion barriers in the mode of action. Furthermore, the N-CECM remained intact without degradation for more than 4 weeks in vivo and exerted anti-adhesion effects for a long time. This study demonstrated that PLL10 surface modification rendered a neutral charge to the polymer on the extracellular matrix surface, thereby inhibiting cell and tissue adhesion. Furthermore, this study suggests a means to modify extracellular matrix surfaces to meet the specific requirements of the target tissue in preventing post-surgical adhesions.

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Inhibitory Effect of Topical Cartilage Acellular Matrix Suspension Treatment on Neovascularization in a Rabbit Corneal Model

Cited by 8 publications

References 50 publications

Effect of detergent type on the performance of liver decellularized extracellular matrix-based bio-inks

Effect of detergent type on the performance of liver decellularized extracellular matrix-based bio-inks

Effect of glutaraldehyde‐crosslinked cartilage acellular matrix film on anti‐adhesion and nerve regeneration in a rat sciatic nerve injury model

Anti‐adhesive effect of chondrocyte‐derived extracellular matrix surface‐modified with poly‐L‐lysine

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