Inhibitory Effect of the Intravenous Anesthetic, Ketamine, on Rat Mesangial Cell Proliferation

Jimi, Nobuo; Segawa, Keiko; Minami, Kouichiro; Sata, Takeyoshi; Shigematsu, Akio

doi:10.1097/00000539-199701000-00034

Cited by 21 publications

(19 citation statements)

References 19 publications

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“…Kohno et al [24]reported that ANP-induced cGMP elevation suppresses PDGF-induced MC proliferation via protein kinase C (PKC) inhibition. Jimi et al [17]showed that ketamine, an intravenous anesthetic, inhibited MC proliferation via protein kinase A (PKA) modulation. Consequently, it seems as though the protein kinase phosphorylation pathway plays a key role in controlling MC proliferation via cGMP elevation.…”

Section: Discussionmentioning

confidence: 99%

“…Rat glomerular MC were cultured as described previously [15, 16, 17]. Briefly, glomeruli were isolated and prepared from 100- to 150-gram male Wistar rats by the sieving method.…”

Section: Methodsmentioning

confidence: 99%

“…The assay for [ 3 H]thymidine incorporation into rat MC was performed as reported previously [15, 16, 17]. Rat MC were cultured for 24, 48 or 72 h at 37°C (95% O 2 /5% CO 2 ) with or without additives in RPMI 1640 containing 4% FBS, in 96-well flat-bottomed microtiter plates (Falcon TM , Becton Dickinson).…”

Section: Methodsmentioning

confidence: 99%

“…To verify that the assay for [ 3 H]thymidine incorporation reflected the effect of nicorandil on actual cell growth, cell counts were performed [15, 16, 17]. In these experiments 10 5 cells/ml were dispensed into Petri dishes and incubated with various concentrations of nicorandil for 72 h. Then, the cells were dispersed with 0.25% trypsin-1 m M EDTA in PBS and counted with a hemocytometer.…”

Section: Methodsmentioning

confidence: 99%

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Inhibitory Effects of Nicorandil on Rat Mesangial Cell Proliferation via the Protein Kinase G Pathway

Segawa

Minami²,

Shiga³

et al. 2001

Nephron

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We investigated the effects of nicorandil, which is a hybrid between a nitrate and an ATP-sensitive potassium channel (K_ATP) opener, on cultured rat mesangial cell proliferation. Nicorandil (1 µM to 1 mM inhibited [³H]thymidine incorporation into rat mesangial cells in a concentration-dependent manner. Nicorandil (1 µM to 1 mM) also inhibited the number of cells. Nicorandil increased cyclic guanosine 3′,5′-cyclic monophosphate accumulation in mesangial cells. A protein kinase G inhibitor, KT5823, partially eliminated the inhibition of mesangial cell proliferation by nicorandil. Methylene blue, a guanylate cyclase inhibitor, blocked the inhibitory effect of nicorandil on mesangial cell proliferation. We also examined the effects of K_ATP mediators. Cromakalim, a K_ATP activator, and glibenclamide, a K_ATP inhibitor, had little effect on the proliferation of mesangial cells. These results suggest that the inhibitory effects of nicorandil on mesangial cell proliferation are mediated via the protein kinase G pathway.

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Section: Discussionmentioning

confidence: 99%

“…Rat glomerular MC were cultured as described previously [15, 16, 17]. Briefly, glomeruli were isolated and prepared from 100- to 150-gram male Wistar rats by the sieving method.…”

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

See 2 more Smart Citations

Inhibitory Effects of Nicorandil on Rat Mesangial Cell Proliferation via the Protein Kinase G Pathway

Segawa

Minami²,

Shiga³

et al. 2001

Nephron

Self Cite

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show abstract

“…Rat glomerular MC were cultured as described previously (Segawa et al 1996;Jimi et al 1997). Briefly, glomeruli were isolated and prepared from 100 to 150 g male Wistar rats by the sieving method.…”

Section: Methodsmentioning

confidence: 99%

C-type natriuretic peptide inhibits rat mesangial cell proliferation by a phosphorylation-dependent mechanism

Segawa

Minami

Jimi

et al. 1997

Naunyn-Schmiedeberg's Arch Pharmacol

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We studied the effects of C-type natriuretic peptide (CNP) on rat cultured mesangial cell proliferation. (1) Exposure to CNP (10 nM-1 microM for 72 h) inhibited [3H]thymidine incorporation into mesangial cells in a concentration-dependent manner. Atrial natriuretic peptide (1 nM-1 microM), a peptide related to CNP, also decreased [3H]thymidine incorporation into these cells in a concentration-dependent manner. (2) Both CNP (10 nM- microM) and atrial natriuretic peptide (10 nM-1 microM) also decreased mesangial cell number. (3) The cyclic GMP analog, 8-bromo-cyclic GMP (100 microM and 1 microM), mimicked the inhibitory effects of CNP and atrial natriuretic peptide on [3H]thymidine incorporation into mesangial cells, whereas inhibitors of protein kinase C, protein kinase A, and protein kinase G reduced the effect of both natriuretic peptides. Moreover, the phosphatase inhibitor, calyculin A, increased [3H]thymidine incorporation into mesangial cells. (4) CNP and atrial natriuretic peptide decreased interleukin-1-, interleukin-6-, platelet derived growth factor-, angiotensin II-induced [3H]thymidine incorporation into mesangial cells. These results suggest that CNP exerts inhibitory effects on mesangial cell proliferation and that this effects depend on protein phosphorylation pathways.

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Biliary clearance of bromosulfophthalein in anesthetized and freely moving conscious rat

Park

Shim

et al. 2009

Biopharm & Drug Disp

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The aim of this study was to investigate the effect of anesthesia on the pharmacokinetics of bromosulfophthalein (BSP) with focus on biliary clearance. The plasma concentration profile and biliary clearance of intravenously administered BSP was compared in conscious freely moving bile duct catheterized rats and rats anesthetized with ketamine or Zoletil. The plasma concentration of BSP in conscious rats was similar to that of anesthetized rats, irrespective of the anesthetic used. There was no significant difference in the volume of distribution, total body clearance and mean residence time of BSP between the groups. The biliary clearance of BSP in rats anesthetized using ketamine or Zoletil was also similar to that of conscious rats. Only bile flow was increased under anesthetization compared with conscious rats. These results demonstrate that the pharmacokinetics of BSP, including biliary clearance, in ketamine or Zoletil anesthetized rats is virtually identical to that in conscious rats and it may be related to blood flow limited hepatic disposition of BSP. Furthermore, they suggest the conscious rat model does not offer methodological advantage and the anesthesia model is suitable for a realistic approximation of the hepatobiliary transport of BSP.

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Inhibitory Effect of the Intravenous Anesthetic, Ketamine, on Rat Mesangial Cell Proliferation

Cited by 21 publications

References 19 publications

Inhibitory Effects of Nicorandil on Rat Mesangial Cell Proliferation via the Protein Kinase G Pathway

Inhibitory Effects of Nicorandil on Rat Mesangial Cell Proliferation via the Protein Kinase G Pathway

C-type natriuretic peptide inhibits rat mesangial cell proliferation by a phosphorylation-dependent mechanism

Biliary clearance of bromosulfophthalein in anesthetized and freely moving conscious rat

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