Inhibitory effect of Sargassum polysaccharide on oxidative stress induced by infectious bursa disease virus in chicken bursal lymphocytes

Zhang, Ling; Hu, Ting‐Jun; Liu, Hongli; Shuai, Xue-Hong

doi:10.1016/j.ijbiomac.2011.06.019

Cited by 24 publications

(10 citation statements)

References 59 publications

(65 reference statements)

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“…Activities were calculated as means ± SD (n = 3), *P0.05, **P0.01, ***P0.001 vs. control of each time interval ride was noticed previously for other polysaccharides, i.e. the sulfated one extracted from algae (Silva et al, 2012) and Sargassum one (Zhang et al, 2011). In our case, possibly the lower level of MDA after the first day of exposure compared to control could be correlated with the slight increase of GSH concentration and GST activity.…”

Section: Antioxidant Enzymesmentioning

confidence: 88%

Water Soluble <i>Pleurotus ostreatus</i> Polysaccharide Down-Regulates the Expression of MMP-2 and MMP-9 in Caco-2 Cells

Cojocaru

Radu

Bodea

et al. 2013

Not Bot Hort Agrobot Cluj

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Many polysaccharides and polysaccharide-protein complexes isolated from mushrooms have immunomodulatory and anti-cancer effects. Our aim was to study the regulatory mechanisms of Caco-2 cell response to water soluble P. ostreatus polysaccharide extract up to 72 hours. Specific enzymatic activities were assessed by kinetic measurements. The reduced glutathione content and the lipid peroxidation level were also analyzed. Protein expression of several heat shock proteins, Bcl-2 and metalloproteinases 2 and 9 were revealed by Western blot. Gelatin zymography assay was used to evaluate the MMP-2 and MMP-9 activities. Until the third day of exposure the total SOD activity decreased continuously by 30%, whereas GST and GR ones diminished by 17% respectively 30.5% compared to control. No significant changes were observed in CAT and G6PDH specific activities as well as in GSH and MDA concentration. After the third day of exposure a significant up-regulation of Hsp60 and Hsp90 expression and a down-regulation of Hsp70 one were registered. Bcl-2 protein levels were down-regulated by 50% in the first day of treatment but increased after 3 days. MMP-2 and 9 secretion in the culture medium was significantly reduced suggesting a diminished ability of invasion of colon cancer cells. Our data revealed that in vitro treatment with P. ostreatus aqueous polysaccharide extract does not induce apoptosis in Caco-2 cell line but it could inhibit the invasion of colon cancer cells through the basement membrane.

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Section: Antioxidant Enzymesmentioning

confidence: 88%

Water Soluble <i>Pleurotus ostreatus</i> Polysaccharide Down-Regulates the Expression of MMP-2 and MMP-9 in Caco-2 Cells

Cojocaru

Radu

Bodea

et al. 2013

Not Bot Hort Agrobot Cluj

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“…Antioxidant effect of plant extracts in chicken lymphocytes has been reported in other studies. Zhang et al observed that Sargassum polysaccharide inhibited oxidative stress induced by infectious bursa disease virus in bursal lymphocytes of chickens [50]. Lv et al found that a polysaccharide from Agaricus blazei Murill had antioxidant effect in chicken peripheral blood lymphocytes treated with cadmium [51].…”

Section: Discussionmentioning

confidence: 99%

Protective Effect of Ginsenoside Rg1 on Oxidative Damage Induced by Hydrogen Peroxide in Chicken Splenic Lymphocytes

Wang

et al. 2019

Oxidative Medicine and Cellular Longevity

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Previous investigation showed that ginsenoside Rg1 (Rg1) extracted from Panax ginseng C.A. Mey has antioxidative effect on oxidative stress in chickens. The present study was designed to investigate the protective effects of Rg1 on chicken lymphocytes against hydrogen peroxide-induced oxidative stress and the potential mechanisms. Cell viability, apoptotic cells, malondialdehyde, activity of superoxide dismutase, mitochondrial membrane potential, and [Ca2+]i concentration were measured, and transcriptome analysis and quantitative real-time polymerase chain reaction were used to investigate the effect of Rg1 on gene expression of the cells. The results showed that treatment of lymphocytes with H2O2 induced oxidative stress and apoptosis. However, pretreatment of the cells with Rg1 dramatically enhanced cell viability, reduced apoptotic cells, and decreased oxidative stress induced by H2O2. In addition, Rg1 reduced these H2O2-dependent decreases in mitochondrial membrane potential and reversed [Ca2+]i overload. Transcriptome analysis showed that 323 genes were downregulated and 105 genes were upregulated in Rg1-treated cells. The differentially expressed genes were involved in Toll-like receptors, peroxisome proliferator-activated receptor signaling pathway, and cytokine-cytokine receptor interaction. The present study indicated that Rg1 may act as an antioxidative agent to protect cell damage caused by oxidative stress via regulating expression of genes such as RELT, EDA2R, and TLR4.

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“…The primary replication site of IBDV is the bursa of Fabricius, where sever destruction of the B lymphocytes causes significant impairment of the antibody response [132]. Infectious bursal disease virus (IBDV) infection of bursal lymphocytes increases intracellular ROS levels, decreases the GSH content and activities of GPx and SOD [134], and increases serum levels of lipid peroxidation (Table 1) [135]. The increased level of ROS may be involved in the shutoff cellular protein synthesis [136], because ROS are involved in the activation of the protein kinase R pathway [137], leading to cell death [136, 138].…”

Section: Avian Virus-induced Oxidative Stress and Antioxidantsmentioning

confidence: 99%

“…Stable overexpression of ORAOV1 is involved in the resistance to oxidative stress [139] which may decrease the IBDV-induced apoptosis [140]. These IBDV-induced oxidative stress and mortality can be reduced by Sargassum polysaccharide, Ginsenoside Rg1, and vitamin E supplementation (Table 1) [134, 135, 141, 142].…”

Section: Avian Virus-induced Oxidative Stress and Antioxidantsmentioning

confidence: 99%

Oxidative Stress in Poultry: Lessons from the Viral Infections

Rehman

Chen

Sun

et al. 2018

Oxidative Medicine and Cellular Longevity

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Reactive species (RS), generally known as reactive oxygen species (ROS) and reactive nitrogen species (RNS), are produced during regular metabolism in the host and are required for many cellular processes such as cytokine transcription, immunomodulation, ion transport, and apoptosis. Intriguingly, both RNS and ROS are commonly triggered by the pathogenic viruses and are famous for their dual roles in the clearance of viruses and pathological implications. Uncontrolled production of reactive species results in oxidative stress and causes damage in proteins, lipids, DNA, and cellular structures. In this review, we describe the production of RS, their detoxification by a cellular antioxidant system, and how these RS damage the proteins, lipids, and DNA. Given the widespread importance of RS in avian viral diseases, oxidative stress pathways are of utmost importance for targeted therapeutics. Therefore, a special focus is provided on avian virus-mediated oxidative stresses. Finally, future research perspectives are discussed on the exploitation of these pathways to treat viral diseases of poultry.

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Inhibitory effect of Sargassum polysaccharide on oxidative stress induced by infectious bursa disease virus in chicken bursal lymphocytes

Cited by 24 publications

References 59 publications

Water Soluble <i>Pleurotus ostreatus</i> Polysaccharide Down-Regulates the Expression of MMP-2 and MMP-9 in Caco-2 Cells

Water Soluble <i>Pleurotus ostreatus</i> Polysaccharide Down-Regulates the Expression of MMP-2 and MMP-9 in Caco-2 Cells

Protective Effect of Ginsenoside Rg1 on Oxidative Damage Induced by Hydrogen Peroxide in Chicken Splenic Lymphocytes

Oxidative Stress in Poultry: Lessons from the Viral Infections

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