Inhibitory Effect of Red Bean (Phaseolus angularis) Hot Water Extracts on Oxidative DNA and Cell Damage

Abstract

-In this study, we evaluated the protective effects of the hot water extract from red bean (Phaseolus angularis) against oxidative DNA and cell damage induced by hydroxyl radical. The antioxidant activities were evaluated by hydroxyl radical and hydrogen peroxide scavenging assay, and Fe 2+ -chelating assay. Although the extract with hot water didn't scavenge the hydroxyl radical, it removed and chelated hydrogen peroxide and ferrous iron necessary for the induction of hydroxyl radical by 71% and 64% at 200 μg/ml, respectively. Its protective effect on oxidative DNA damage was carried using ψX-174 RF I plasmid DNA comparing the conversion level of supercoiled form of the plasmid DNA into open-circular form and linear form and the expression level of phospho-H2AX in NIH 3T3 cells. In ψX-174 RF I plasmid DNA cleavage assay, it inhibited oxidative DNA damage by 96% at 200 μg/ml. Also, it decreased the expression of phospho-H2AX by 50.1% at 200 μg/ml. Its protective effect against oxidative cell damage was measured by MTT assay and the expression level of p21 protein in NIH 3T3 cells. In MTT assay for the protective effect against the oxidative cell damage, it inhibited the oxidative cell death and the abnormal cell growth induced by hydroxyl radical. Also, it inhibited p21 protein expression by 98% at 200 μg/ml. In conclusion, the results of the present studies indicate that hot water extract from red bean exhibits antioxidant properties and inhibit oxidative DNA damage and the cell death caused by hydroxyl radical.

A study of anti‐wrinkle functions and improvement of cream with Phaseolus angularis

“…Park reported that the in vitro DNA cleavage, DNA migration and H2AX phosphorylation inhibitory activity of the Phaseolus angularis hydrothermal extract showed high activity, which effectively inhibited DNA damage by radicals. Also, looking at the effect of lipid peroxidation and p21 expression rate on cell oxidative damage, it is thought to protect cells from oxidative stress caused by radicals as it is very effective in inhibiting lipid peroxidation and p21 expression rate [34].…”

Efficacy and Safety of Phaseolus angularis L for. as Anti- aging Therapy in Healthy Skin

“…reported that the in vitro DNA cleavage, DNA migration, and H2AX phosphorylation inhibitory activity of the Phaseolus angularis hydrothermal extract showed high activity, which effectively inhibited DNA damage by radicals. Also, looking at the effect of lipid peroxidation and p21 expression rate on cell oxidative damage, it is thought to protect cells from oxidative stress caused by radicals as it is very effective in inhibiting lipid peroxidation and p21 expression rate [34]. Therefore, Phaseolus species have fewer side effects and less harm to the Earth than chemical products and have excellent antioxidant properties, so they can be a good resource for cosmetics.…”

Effect of Hydropsyche kozhantschikovi Extracts on Oxidative Stress

“…최근 청동풍 뎅이(Anomala albopilosa) 추출물이 DPPH의 free radical, xanthine oxidase 및 superoxide radical에 대한 소거 활성이 높게 나타나 우수한 항산화물질을 갖고 있다고 하였으며 [25], 칠성 무당벌레(Coccinella septempunctata)를 이용하여 DPPH, FRAP, linoleic acid 산화 억제 실험을 실시한 결과 높은 항산 화 활성을 보여 산업적 활용가능성이 있다고 보고된 바 있다 [2]. 또한 섬서구 메뚜기(Atractomorpha lata)와 벼메뚜기(Oxya japonica) 및 끝검은 메뚜기(Stethophyma magister)의 항산화능 과 과산화수소에 대한 세포 보호능 연구 결과, 모든 메뚜기류 에서 높은 항산화 · 세포보호 활성이 조사되어 천연 항산화 기능성 소재로서 이용가능성이 보고된 바 있다 [17].…”