A study of anti‐wrinkle functions and improvement of cream with Phaseolus angularis

Abstract

Chronic exposure to ultraviolet (UV) radiation from sunlight accelerates skin ageing, which is followed by harsh, thick, dry and loose conditions. One of the most demonstrative symptoms is deep wrinkles induced by skin barrier disruption. Our previous research showed that Phaseolus angularis seed extract (PASE) effectively inhibits skin ageing through UVB protection in HaCaT cells by suppressing skin damage. However, its efficacy has not been evaluated in clinical trials so far. PASE cream's effectiveness was initially tested on the artificial skin model, revealing an increase in filaggrin and defence against skin damage. Based on these results, in this single‐centred, randomized, double‐blind study, we investigated the anti‐ageing effect of PASE in human eye wrinkle areas. For these 21 healthy adult women aged 30 to 59, a PASE cream was applied to the right eye wrinkle area and a placebo to the left eye wrinkle area twice a day (morning and evening) for 12 weeks. The change in thick, deep crease wrinkles around the eyes was confirmed by visual evaluation, skin measurements and a questionnaire. As a result, the surface roughness (R1), maximum roughness (R2), average roughness (R3), smoothness depth (R4) and arithmetic mean roughness (R5) values in the group using the PASE cream all decreased. Particularly, R1, R4 and R5 significantly decreased by 18.1%, 18.6% and 25.0%, respectively. Subjects who applied PASE cream also experienced an improvement in skin moisture nearly twice the time compared to the placebo group. In addition, no participants reported side effects. Our study showed that PASE cream led to clinically significant levels of wrinkle improvement. In conclusion, as PASE is a natural, safe food with no side effects, it can be a good resource for natural anti‐wrinkle functional cosmetics in the future.

Clinical Efficacy in Skin Hydration and Reducing Wrinkles of Nanoemulsions Containing Macadamia integrifolia Seed Oil

“…These parameters are measured in arbitrary units (A.U. ), with lower values indicating improved skin wrinkles [ 41 ]. The findings indicate that both the conventional emulsion and nanoemulsion of M. integrifolia oil could significantly reduce the skin volume and R1–R5, indicating uniform effects across multiple variables and reinforcing the reliability of the findings.…”

“…Besides this, both formulations could enhance skin hydration Aside from the skin hydration enhancement effect, M. integrifolia oil formulations were found to reduce the skin wrinkles and roughness (Figure 6). In brief, skin roughness (R1) signifies the difference between the highest and lowest peaks in wrinkles, with maximum roughness (R2) representing the largest R1 values and average roughness (R3) being the arithmetic average of R1 values within these sections [41]. The smoothness depth (R4) represents the average depth of skin wrinkles, calculated by dividing the area formed by the wrinkle roughness by the length of the horizontal line after the integration of the highest peak and horizontal line, while the arithmetic average roughness (R5) is determined by integrating the area formed by the middle line of wrinkle roughness and dividing it by the length of the middle line, indicating the arithmetic mean roughness [41].…”