Inhibitory effect of punicalagin on lipopolysaccharide-induced neuroinflammation, oxidative stress and memory impairment via inhibition of nuclear factor-kappaB

Kim, Young Eun; Hwang, Chul Ju; Lee, Hee Pom; Kim, Chun Sik; Son, Dong Ju; Ham, Young Wan; Hellström, Mats; Han, Sang‐Bae; Kim, Hee Sik; Park, Eun Kyung; Hong, Jin Tae

doi:10.1016/j.neuropharm.2017.01.025

Cited by 113 publications

(99 citation statements)

References 52 publications

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“…NF-κB can regulate many processes involved in immune function and inflammation (Ho et al, 2012; Kim et al, 2017). Our results showed that after infrasound exposure, the expression of p-IκBα and nuclear NF-κB p65 was increased, suggesting that infrasound may activate NF-κB through degradation of IκBs and subsequent translocation of NF-κB p65 subunit into the nuclei, finally activating transcription of target genes, including various proinflammatory cytokines (such as TNF-α, IL-1β, IL-18, IL-6, and IFN-γ).…”

Section: Discussionmentioning

confidence: 99%

Inhibitive Effects of FGF2/FGFR1 Pathway on Astrocyte-Mediated Inflammation in vivo and in vitro After Infrasound Exposure

Shi

Xiao

et al. 2018

Front. Neurosci.

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Infrasound, a kind of ambient noise, can cause severe disorders to various human organs, specially to central nervous system (CNS). Our previous studies have shown that infrasound-induced CNS injury was closely related with astrocytes activation and astrocytes-mediated neuroinflammation, but the underlying molecular mechanisms are still largely unclear. FGF2/FGFR1 (Fibroblast growth factor 2/Fibroblast growth factor receptor 1) pathway was reported to play an important role in anti-inflammation in CNS disorders. To further study the possible roles of FGF2/FGFR1 pathway in infrasound-induced CNS injury, here we exposed Sprague-Dawley rats or cultured astrocytes to 16 Hz, 150 dB infrasound, and explored the effects of FGF2 on infrasound-induced astrocytes activation and neuroinflammation. Western blotting, immunofluorescence and liquid chip method were used in this experiment. Our results showed that after 3- or 7-day exposure (2 h/day) of rats as well as 2 h exposure of cultured astrocytes to 16 Hz, 150 dB infrasound, astrocyte-expressed FGFR1 was downregulated in vivo and in vitro. FGF2 pretreatment not only inhibited infrasound-induced astrocyte activation in rat hippocampal CA1 region, but also reduced the levels of pro-inflammatory cytokines, such as TNF-α, IL-1β, IL-18, IL-6, and IFN-γ in vitro and in vivo. However, FGF2 significantly upregulated the expression of FGFR1. Furthermore, we showed that FGF2 could attenuate IκBα phosphorylation, NF-κB p65 translocation, pro-inflammatory cytokines levels, and neuronal loss in the CA1 region induced by infrasound. On the contrary, PD173074, a special antagonist of FGFR1, could reverse the effects above in vitro and in vivo. Taken together, our findings showed that FGF2/FGFR1 pathway may exert inhibitive effects on astrocyte-mediated neuroinflammation in vitro and in vivo after infrasound exposure.

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Section: Discussionmentioning

confidence: 99%

Inhibitive Effects of FGF2/FGFR1 Pathway on Astrocyte-Mediated Inflammation in vivo and in vitro After Infrasound Exposure

Shi

Xiao

et al. 2018

Front. Neurosci.

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“…PCG decreased protein expression of NFκB-p65 by reducing phosphorylation of IκBα [30]. Also, Kim and his colleagues reported inhibitory effect of PCG on lipopolysaccharide induced inflammation via inhibition of NFκB [31].…”

mentioning

confidence: 92%

Gastroprotective Effect of Punicalagin against Ethanol-Induced Gastric Ulcer: The Possible Underlying Mechanisms

Mohamed¹,

Salahuddin²

2017

Biomark J

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Gastric ulcer is the most digestive disease found in clinical practice. Punicalagin (PCG) found in pomegranate juice has antioxidant and tissue repair properties. Therefore, the aim of this study was to investigate punicalagin's gastroprotective effect against ethanol-induce gastric injury. Four groups of rats; first group served as control, group 2: Treated with absolute ethanol (5 ml/kg, po), group 3: Rats were pre-treated with ranitidine (as a reference drug) (50 mg/kg, po) before ethanol and group 4: Pre-treated with PCG (4 mg/kg, po) before ethanol. Pretreatment with PCG reduced ulcer index and histopathological changes, oxidative stress induced by ethanol while it elevated antioxidant activity. Although, it down regulated Tumor Necrosis Factor (TNF-α) gene expression and mucosal levels of inflammatory cytokines; TNF-α, Interleukin (IL-1β) and Interferon Gamma (IFNγ), it up regulated mucosal level of IL-10. Also, it reduced mucosal Nuclear Factor Kappa B (NFκB) protein expression, mylopeoxidase and caspase 3 activities as well as gene expression of caspase 9 while it elevated antiapoptotic B-Cell Lymphoma 2 (Bcl-2) gene expression, mucosal nitric oxide and mucin content. However, it had negative action on prostaglandin E2 and acid secretions. These findings indicate gastroprotective effects of punicalagin against ethanol induced gastric ulcer through suppression of mucosal oxidative stress and inflammation through NFκB pathway as well cytoprotective defences independent on prostaglandin E2 and acid secretions.

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“…Punicalagin is reported to have anti-cancer, antiatherosclerotic and antiobesity properties (10). Although many researches have reported the anti-inflammatory feature of pomegranate leaf extract, effects of punicalagin obtained from pomegranate leaf extract is not known clearly (11,12). Better discerning of the anti-inflammatory effects of punicalagin may lead to further development of new strategies for dysregulated inflammatory prevention at many sites.…”

Section: Introductionmentioning

confidence: 99%

Punicalagin isolated from Punica granatum husk can decrease the inflammatory response in RAW 264.7 macrophages

Berköz¹,

Allahverdiyev²

2017

Eastern J Med

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Inhibitory effect of punicalagin on lipopolysaccharide-induced neuroinflammation, oxidative stress and memory impairment via inhibition of nuclear factor-kappaB

Cited by 113 publications

References 52 publications

Inhibitive Effects of FGF2/FGFR1 Pathway on Astrocyte-Mediated Inflammation in vivo and in vitro After Infrasound Exposure

Inhibitive Effects of FGF2/FGFR1 Pathway on Astrocyte-Mediated Inflammation in vivo and in vitro After Infrasound Exposure

Gastroprotective Effect of Punicalagin against Ethanol-Induced Gastric Ulcer: The Possible Underlying Mechanisms

Punicalagin isolated from Punica granatum husk can decrease the inflammatory response in RAW 264.7 macrophages

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