Inhibitory effect of pituitary adenylate cyclase activating polypeptide on the initial stages of rat follicle development

Latini, Stefania; Chiarpotto, M.; Muciaccia, Barbara; Vaccari, Sergio; Barberi, Marzia; Guglielmo, Maria Cristina; Stefanini, Mario; Cecconi, Sandra; Canipari, Rita

doi:10.1016/j.mce.2010.01.040

Cited by 10 publications

(5 citation statements)

References 21 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Indeed, several studies of the role of PACAP in the gonads have implicated PACAP in follicular development Latini et al, 2010), antiapoptotic effects (Morelli et al, 2008) and implantation (Isaac et al, 2008). However, the physiological function of PACAP in mammalian oocyte during meiotic maturation remains unknown.…”

Section: Introductionmentioning

confidence: 99%

Pituitary Adenylate Cyclase-activating Polypeptide (PACAP) Treatment during Pre-maturation Increases the Maturation of Porcine Oocytes Derived from Small Follicles

Hyun

2018

J Anim Reprod Biotechnol

View full text Add to dashboard Cite

Cellular cyclic adenosine-3' 5'-monophosphate (cAMP) modulator is known as meiotic inhibitor and can delays spontaneous maturation in IVM experiment. Among many cAMP modulators, the role of Pituitary adenylate cyclase activating polypeptide (PACAP) on IVM isn't known. The purpose of this study is to improve the maturation of oocytes derived from follicles ≤ 3 mm in diameter through PACAP as meiotic inhibitor during pre-in vitro maturation (pre-IVM). First, we checked PACAP and its receptors in cumulus cells and, to establish the optimal phase and concentration of PACAP for pre-IVM, we conducted chromatin configuration assessments. As a result, the rate of GV (Germinal Vesicle) according to duration of pre-IVM was significantly decreased 12 h and 18 h after IVM (87.1 and 84.1%, respectively) compared to 0 h (99.4%). When COC was cultured for 18 h, the GV rate in the 1 µM of PACAP treatment group (82.1%) was significantly higher than any other PACAP treatment groups (60.5, 64.1, 74.4 and 69.9 %, respectively). So, we divided into four groups as follows; MF (the conventional IVM group, obtained from follicle from 3 to 6 mm in diameter), SF (the conventional IVM group, obtained from follicle ≤ 3mm in diameter), Pre-SF(-)PACAP (IVM group including 18 h pre-IVM without 1 µM of PACAP, obtained from follicle ≤ 3mm in diameter) and Pre-SF(+)PACAP (IVM group including 18 h pre-IVM with 1 µM of PACAP, obtained from follicle ≤ 3mm in diameter). To examine the effect of PACAP during pre-IVM, we investigated analysis of nuclear maturation, intracellular glutathione (GSH) and reactive oxygen species (ROS) levels. In cumulus cells, PACAP receptors, ADCYAP1R1 and VIPR1 were detected but were not detected in oocytes. After IVM, the Pre-SF(+)PACAP had the highest Metaphase II rate (91.7%) among all groups (P<0.05). The GSH levels in the MF and Pre-SF(+)PACAP were significantly higher than in the other groups (P<0.05) and ROS levels was no significant difference among all groups. In conclusion, these results indicated that even though the oocytes were derived from SF, pre-IVM application of PACAP improved meiotic and cytoplasmic maturation by regulating intracellular oxidative stress.

show abstract

Section: Introductionmentioning

confidence: 99%

Pituitary Adenylate Cyclase-activating Polypeptide (PACAP) Treatment during Pre-maturation Increases the Maturation of Porcine Oocytes Derived from Small Follicles

Hyun

2018

J Anim Reprod Biotechnol

View full text Add to dashboard Cite

show abstract

“…Studies have shown that PACAP/PAC1R can also inhibit the secretion of FSH through the HPO axis [30]. For example, PACAP can stimulate the transcription of follistatin (Fst) and then inhibit the secretion of FSH [86]. This result indicates that the relationship may be complicated between PACAP/PAC1R and the synthesis of FSH, and needs to be studied further.…”

Section: Discussionmentioning

confidence: 99%

Effect of PACAP/PAC1R on Follicle Development of Djungarian Hamster (Phodopus sungorus) with the Variation of Ambient Temperatures

Xue

et al. 2023

Biology

View full text Add to dashboard Cite

In Phodopus sungorus, the relationship between pituitary adenylate cyclase-activating polypeptide (PACAP) and its receptor (PAC1R), follicle-stimulating hormone (FSH), and follicle development remains unclear. In this study, we found that the development of growing follicles and antral follicles were inhibited at low (8 °C, 14 °C) and high (29 °C) temperatures. Meanwhile, PACAP/PAC1R expression and follicle-stimulating hormone (FSH) serum concentration significantly decreased during ambient temperatures of 8 °C, 14 °C and 29 °C compared to 21 °C. Thus, ambient temperature may influence the expression of PACAP/PAC1R and the synthesis of FSH for involvement in follicle development. Moreover, PACAP/PAC1R had major functional elements including PKA/PKG and PKC phosphorylation sites, which may involve in the pathway of FSH synthesis through cAMP-PKA and its downstream signal pathway. Moreover, there was a significant positive correlation between the expression levels of PACAP/PAC1R and the number of the growing and antral follicles, as well as the serum FSH concentration and the number of antral follicles. However, there was no significant correlation between the expression levels of PACAP/PAC1R and the serum FSH concentration, indicating a complicated pathway between PACAP/PAC1R and FSH. In conclusion, ambient temperature affects the expression of PACAP/PAC1R and the serum FSH concentration. The expression of PACAP/PAC1R and the serum FSH concentration are correlated with follicle development, which implies that they are involved in follicle development, which will ultimately influence the reproduction of Phodopus sungorus. This study can lay the foundation for future investigation on the regulation mechanism of reproduction in Phodopus sungorus.

show abstract

“…PACAP also inhibited the growth of preantral follicles. These observations indicate an important role of PACAP in follicular recruitment (Latini et al, 2010). On the other hand, at later stages, PACAP can prime immature follicles (Gras et al, 2005).…”

Section: Pacap In Mammalian Follicular Developmentmentioning

confidence: 91%

“…Fine balances between inhibitory and stimulatory signals regulate follicular recruitment. PACAP has been shown to inhibit primordial to primary follicle transition (Latini et al, 2010); PACAP did not influence granulosa cell viability at these stages but inhibited proliferation. PACAP also inhibited the growth of preantral follicles.…”

Section: Pacap In Mammalian Follicular Developmentmentioning

confidence: 94%

See 1 more Smart Citation

Role of PACAP in Female Fertility and Reproduction at Gonadal Level – Recent Advances

et al. 2012

View full text Add to dashboard Cite

Pituitary adenylate cyclase activating polypeptide (PACAP) is a pleiotropic neuropeptide, first isolated from hypothalamic extracts, but later shown in peripheral organs, such as endocrine glands, gastrointestinal system, cardiovascular system, and reproductive organs. PACAP plays a role in fertility and reproduction. Numerous studies report on the gonadal regulatory effects of PACAP at hypothalamo-hypophyseal levels. However, the local effects of PACAP at gonadal levels are also important. The present review summarizes the effects of PACAP in the ovary. PACAP and its receptors are present in the ovary, and PACAP plays a role in germ cell migration, meiotic division, follicular development, and atresia. The autocrine-paracrine hormonal effects seem to play a regulatory role in ovulation, luteinization, and follicular atrophy. Altogether, PACAP belongs to the ovarian regulatory peptides.

show abstract

Inhibitory effect of pituitary adenylate cyclase activating polypeptide on the initial stages of rat follicle development

Cited by 10 publications

References 21 publications

Pituitary Adenylate Cyclase-activating Polypeptide (PACAP) Treatment during Pre-maturation Increases the Maturation of Porcine Oocytes Derived from Small Follicles

Pituitary Adenylate Cyclase-activating Polypeptide (PACAP) Treatment during Pre-maturation Increases the Maturation of Porcine Oocytes Derived from Small Follicles

Effect of PACAP/PAC1R on Follicle Development of Djungarian Hamster (Phodopus sungorus) with the Variation of Ambient Temperatures

Role of PACAP in Female Fertility and Reproduction at Gonadal Level – Recent Advances

Contact Info

Product

Resources

About