Inhibitory effect of nucleotides on acetylcholine esterase activity and its microflow-based actuation in blood plasma

Deshwal, Akshi; Gill, Arshdeep Kaur; Nain, Surajmal; Patra, Debabrata; Maiti, Subhabrata

doi:10.1039/d2cc00029f

Cited by 8 publications

(6 citation statements)

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“…2b and c). 8 Herein, also the experimental results indicated no disruption of the tertiary structure in the presence of carbohydrates (100 mM), rather a slight stabilization happens as at 6M GdnCl the shift in the peak maximum is less than that in the case of only ALP, in every case (Fig. 2c).…”

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confidence: 50%

Trade-off between carbohydrates and metal ions regulates the chemotactic directionality of alkaline phosphatase

2022

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supporting

confidence: 50%

Trade-off between carbohydrates and metal ions regulates the chemotactic directionality of alkaline phosphatase

2022

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“…The applicability of such enzyme-powered micropumps as biomedical devices has been demonstrated by the triggered release of insulin in response to glucose concentration. Several reports of toxin detection have also been shown where catalase, urease, and acetylcholinesterase-powered micropumps were used as microscale sensors. − In this case, the fluid flow velocity was greatly diminished by the presence of inhibitors, which altered the enzymatic reaction rate. Additionally, for various applications that require controlled dosing or mixing, it is also important to have control over the flow velocity of a self-powered enzyme micropump.…”

Section: Introductionmentioning

confidence: 99%

Supramolecular Modulation of Fluid Flow in a Self-Powered Enzyme Micropump

Agashe,

Saroha,

Agasti

et al. 2024

Langmuir

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Regulating macroscopic fluid flow by catalytic harnessing of chemical energy could potentially provide a solution for powerless microfluidic devices. Earlier reports have shown that surface-anchored enzymes can actuate the surrounding fluid in the presence of the respective substrate in a concentration-dependent manner. It is also crucial to have control over the flow speed of a self-powered enzyme micropump in various applications where controlled dosing and mixing are required. However, modulating the flow speed independent of the fuel concentration remains a significant challenge. In a quest to regulate the fluid flow in such a system, a supramolecular approach has been adopted, where reversible regulation of enzyme activity was achieved by a two-faced synthetic receptor bearing sulfonamide and adamantane groups. The bovine carbonic anhydrase (BCA) enzyme containing a single binding site favorable to the sulfonamide group was used as a model enzyme, and the enzyme activity was inhibited in the presence of the two-faced inhibitor. The same effect was reflected when the immobilized enzyme was used as an engine to actuate the fluid flow. The flow velocity was reduced up to 53% in the presence of 100 μM inhibitor. Later, upon addition of a supramolecular "host" CB[7], the inhibitor was sequestered from the enzyme due to the higher binding affinity of CB [7] with the adamantane functionality of the inhibitor. As a result, the flow velocity was restored to ∼72%, thus providing successful supramolecular control over a self-powered enzyme micropump.

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“…The progress of Aβ formation occurs in two steps: first, β-secretase cleaves APP to form C99; next, γ-secretase continues cleaving C99 to generate Aβ of a different length, consisting mainly of Aβ 40 and Aβ 42 , as presented in Figure 2 . An insignificant number of researchers have studied acetylcholine esterase, acetyl choline, and choline as biomarkers associated with AD [ 2 , 6 , 7 , 8 , 9 ]. Biosensors capable of detecting these biomarkers for diagnosis and early warning to support AD treatment have been intensively researched currently.…”

Section: Introductionmentioning

confidence: 99%

“…choline as biomarkers associated with AD [2,[6][7][8][9]. Biosensors capable of detecting these biomarkers for diagnosis and early warning to support AD treatment have been intensively researched currently.…”

Section: Introductionmentioning

confidence: 99%

SAM-Support-Based Electrochemical Sensor for Aβ Biomarker Detection of Alzheimer’s Disease

Kim

Cho

2023

Biosensors

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Alzheimer’s disease has taken the spotlight as a neurodegenerative disease which has caused crucial issues to both society and the economy. Specifically, aging populations in developed countries face an increasingly serious problem due to the increasing budget for patient care and an inadequate labor force, and therefore a solution is urgently needed. Recently, diverse techniques for the detection of Alzheimer’s biomarkers have been researched and developed to support early diagnosis and treatment. Among them, electrochemical biosensors and electrode modification proved their effectiveness in the detection of the Aβ biomarker at appropriately low concentrations for practice and point-of-care application. This review discusses the production and detection ability of amyloid beta, an Alzheimer’s biomarker, by electrochemical biosensors with SAM support for antibody conjugation. In addition, future perspectives on SAM for the improvement of electrochemical biosensors are also proposed and discussed.

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Inhibitory effect of nucleotides on acetylcholine esterase activity and its microflow-based actuation in blood plasma

Abstract: The inhibitory effect of nucleotides on the catalytic activity of acetylcholine esterase (AChE) was rationalized and similar inhibition trend was observed when analyzing the macroscopic fluid flow generated by surface...

Cited by 8 publications

References 44 publications

Trade-off between carbohydrates and metal ions regulates the chemotactic directionality of alkaline phosphatase

Trade-off between carbohydrates and metal ions regulates the chemotactic directionality of alkaline phosphatase

Supramolecular Modulation of Fluid Flow in a Self-Powered Enzyme Micropump

SAM-Support-Based Electrochemical Sensor for Aβ Biomarker Detection of Alzheimer’s Disease

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