Trade-off between carbohydrates and metal ions regulates the chemotactic directionality of alkaline phosphatase

Abstract

Herein we report chemotactic behaviour alkaline phosphatase (ALP), in gradient of carbohydrates (glucose, fructose, sucrose) and metal ions, including cofactors (Zn2+, Mg2+) in microfluidic condition. We found ALP migrates marginally...

Self-Regulatory Micro- and Macroscale Patterning of ATP-Mediated Nanobioconjugate

“…Additionally, our finding demonstrates that the diffusiophoretic effect dictates the taxis and zone of nanobioconjugate assembly formation, and this process can be actuated by introduction of the enzyme, ALP. In previous instances, many nonequilibrium and dynamic self-assembly and patterning of nanoparticles or active colloidal matter were exemplified using complementary charge interaction, light, or in combination with lithography and a self-assembly approach. − In fact, all of these have significant applicability in designing plasmonic or SERS-active substrates and dynamic nonlinear oscillatory and catalytic systems or materials. − Furthermore, this manuscript reports diffusiophoretic formation and accumulation of nanobioconjugate in microfluidic conditions, where the growth rate can be manipulated by enzyme–substrate action operating in a gradient. The previous report of “diffusiophoretic banding” was reported with polymer beads in gradient of NaCl, KCl, or LiCl, which exemplifies diffusiophoresis driven large scale mixing, explaining the possible reason behind enhanced migration of particles through coastal aquifers in the salt gradient and specific sedimentation zone relevant to oceanography and estuarine hydrodynamics .…”

A Cautionary Perspective on Hydrogel-Induced Concentration Gradient Generation for Studying Chemotaxis

“…18−20 To observe chemotaxis, it is imperative to establish a concentration gradient of the chemical within controlled environments such as microfluidic systems, hybridization chambers, or Petri dishes. Somasundar et al, 12 Zhao et al, 9 and Deshwal et al 21 preferred microfluidic devices with direct contact between flowing fluids to establish concentration gradients. For these experiments, they used multi-inlet microchannels and observed movements of enzymes or enzyme-coated particles under the established gradients.…”

Simultaneous quantification of serum albumin and gamma globulin using Zn(ii)-metallosurfactant via a coffee ring pattern

“…In previous literature also the compactness of the GGB structure upon binding with nanoparticles was reported. 15 Additionally, the binding constant value for C 16 DPA·Zn 2+ was found to be almost 2–3 orders of magnitude higher than that for CTAB, both for ALB and GGB. In the case of anionic SDS, ALB and GGB binding was the lowest among the other two cationic surfactants.…”