Inhibitory Effect of Natural Phenolic Compounds onAspergillus parasiticusGrowth

Pizzolitto, Romina Paola; Barberis, Carla Lorena; Dambolena, José Sebastián; Herrera, Jimena María; Zunino, María Paula; Magnoli, Carina Elizabeth; Rubinstein, H.R.; Zygadlo, Julio Alberto; Dalcero, Ana María

doi:10.1155/2015/547925

Cited by 39 publications

(27 citation statements)

References 28 publications

(41 reference statements)

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“…Pizzolitto et al [22] also screened strains of the filamentous fungus Aspergillus parasiticus using the phytochemicals: thymol, carvacrol, eugenol, isoeugenol, creosol, m-Creosol, p-Creosol, o-Cresol and phenol. In this study, they found that isoeugenol had a MIC of 206 μg/mL, which was lower than those found in the other compounds tested, showing its potency against the microorganism, including carvacrol.…”

Section: Resultsmentioning

confidence: 99%

In Vitro Antifungal Effect of Isoeugenol Against Penicillium citrinum Strains

Ferreira

Dantas

Silva

et al. 2017

Proceedings of MOL2NET 2017, International Conference on Multidisciplinary Sciences, 3rd Edition

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Graphical AbstractAbstract.Human mycoses have controversial treatment, since the available antifungal drugs besides favoring the appearance of resistant isolates, can present great toxicity to the human organism. This fact has driven the search for more efficient, safe and natural therapeutic alternatives with the objective of reversing this scenario of resistance presented by pathogenic fungi, for this, new natural and effective alternatives, such as the use of plant extracts, natural compounds and semisynthetic, have been extensively investigated for the development of new drugs. In view of this scenario, this study aims to evaluate the antifungal activity of isoeugenol in vitro against strains of Penicillium citrinum. For this, the broth microdilution technique was used to determine the Minimum Inhibitory Concentration (MIC) and Minimum Fungicide Concentration (MFC) of isoeugenol and voriconazole. The concentrations by which the strains were submitted, 1024 μg/mL to 0.5 μg/mL, were obtained by means of serial dilution at a ratio of two, so that in the first row of the plate is the highest concentration and in the last , the lowest concentration. Finally, 10 μL of the fungal inoculum of each isolate was added to the wells, where each strain was placed on a plate MOL2NET, 2017, 3, doi:10.3390/mol2net-03-xxxx 2 column. MIC was defined as the lowest concentration capable of inhibiting fungal growth visually verified by maintaining the original coloration of the medium. After reading the MIC, aliquots of 20 μL of the supernatant from the wells where complete inhibition of fungal growth in the microdilution plates were observed were plated on Sabouraud dextrose agar plates. Plates were incubated, and MFC was considered the lowest concentration at which growth was less than 3 colonies. The assays were performed in triplicate and the geometric mean was calculated. The CIM of isoeugenol varied between 256 and 32 μg/mL, being the highest MIC value for the LM-21 strain and the lowest value for the LM-02, LM-03, LM-08, LM-155, LM-157 and LM-161. The MIC range of voriconazole was 0.5 to 256 μg/mL. The MFC of isoeugenol varied between 64-512 μg/mL and for voriconazole it was 2 μg/mL and above 1024 μg/mL. In this way, we can conclude that isoeugenol presented an antifungal effect, which enables it as a potential antifungal drug, requiring complementary tests that clarify the mechanism of action involved in its antimicrobial activity.

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Section: Resultsmentioning

confidence: 99%

In Vitro Antifungal Effect of Isoeugenol Against Penicillium citrinum Strains

Ferreira

Dantas

Silva

et al. 2017

Proceedings of MOL2NET 2017, International Conference on Multidisciplinary Sciences, 3rd Edition

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“…The radios of the colonies were plotted against time, and a linear regression applied in order to obtain the growth rate as the slope of the line. The effect of each ketone compound on Lag phase for growth, was defined as the time (days) in which each colony reaches 5 mm of diameter [ 15 ].…”

Section: Methodsmentioning

confidence: 99%

“…Modern strategies of biological control of pests are being investigated as alternatives to synthetic pesticides; monoterpenes found mainly as components of essential oils of plants are an alternative. Previous studies reported bioactivities (antifungal, insecticidal and repellence) of terpenes with different structural groups on stored grain pests, with ketones being the most bioactive [ 14 , 15 , 16 , 17 ]. However, little is known about the bioactivity of natural ketone compounds against biotrophic pathogens.…”

Section: Introductionmentioning

confidence: 99%

Bioactivities of Ketones Terpenes: Antifungal Effect on F. verticillioides and Repellents to Control Insect Fungal Vector, S. zeamais

et al. 2015

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Maize is one the most important staple foods in the world. However, numerous pests, such as fungal pathogens, e.g., Fusarium verticillioides, and insects, such as Sitophlilus zeamais, attack maize grains during storage. Many F. verticillioides strains produce fumonisins, one of the most important mycotoxin that causes toxic effects on human and animal health. This situation is aggravated by the insect fungal vector, Sitophlilus zeamais, which contributes to the dispersal of fungal spores, and through feeding damage, provide entry points for fungal infections. The aim of this study was to evaluate in vitro bioassays, the antifungal activity on F. verticillioides M3125 and repellent effects against S. zeamais of ketone terpenes. In addition, we performed Quantitative structure–activity relationship (Q-SAR) studies between physico-chemical properties of ketone terpenes and the antifungal effect. Thymoquinone was the most active compound against F. verticillioides (Minimum Inhibitory Concentration, MIC: 0.87) affecting the lag phase and the growth rate showing a total inhibition of growth at concentration higher than 2 mM (p < 0.05). The Q-SAR model revealed that the antifungal activity of ketone compounds is related to the electronic descriptor, Pi energy. Thymoquinone showed a strong repellent effect (−77.8 ± 8.5, p < 0.001) against S. zeamais. These findings make an important contribution to the search for new compounds to control two stored pests of maize.

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“…According to Pizzolitto et al reported the ability of the organic compounds to perturb the cell membrane integrity. Thus, the mechanism depends on the organic ability to affect the function of cellular lipoprotein membranes 17 . Moreover, refractivity index and molar volume are related to the antifungal effect, indicating that specific interactions between the phenolic compounds and the target receptor could be implicated in the inhibitory activity.…”

Section: Lichen Powder Extractmentioning

confidence: 99%

Antifungal Potential Against Aspergillus Flavus: Secondary Metabolite Compound From Unique Organism of Lichen Teloschistes Flavicans

Maulidiyah¹,

Hasan²,

Irna³

et al. 2018

Int. Res. J. Pharm.

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Lichen is a unique symbiotic organism formed from the mutualism relationship between fungus and algae. The aims of this research are to isolate and to examine antifungal activity of secondary metabolite compound from lichen Teloschistes flavicans. This research was conducted by extracting lichen dried powder using acetone solvent for 3×24 hours. The extract was isolated by Column Chromatography (CC) and Thin Layer chromatography (TLC) with G60 silica gel as stationary phase. Then it was eluted by n-hexane and ethyl acetate solvents in a gradient elution. The isolate was obtained and purified by recrystallization process then determined by using UV-VIS, FTIR, LC-MS, and 1H-NMR. The isolated compound is an orange needle "3-[1'-(2",3"-dihydroxy-phenyl)-propyl]-7hydroxy-chroman-4-one" with the formula of C18H18O5. The antifungal activity test against Aspergillus flavus using disc diffusion method showed that the inhibitory zone was 11 mm in the concentration of 1000 mg mL-1 .

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Inhibitory Effect of Natural Phenolic Compounds onAspergillus parasiticusGrowth

Cited by 39 publications

References 28 publications

In Vitro Antifungal Effect of Isoeugenol Against <em>Penicillium citrinum</em> Strains

In Vitro Antifungal Effect of Isoeugenol Against <em>Penicillium citrinum</em> Strains

Bioactivities of Ketones Terpenes: Antifungal Effect on F. verticillioides and Repellents to Control Insect Fungal Vector, S. zeamais

Antifungal Potential Against Aspergillus Flavus: Secondary Metabolite Compound From Unique Organism of Lichen Teloschistes Flavicans

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